70-Minute Amino Acid
Analysis of
Physiological Samples
Pickering
Laboratories specializes in the manufacturing of
cation-exchange columns and eluants for amino acid
analysis. Post-column derivatization with
Ninhydrin offers unmatched selectivity and
reproducibility of the analysis for most
challenging matrixes.
Pinnacle PCX
post-column derivatization system allows analysts
to combine eluant gradient capabilities of modern
HPLC instruments with column temperature
gradients. We introduce new accelerated Amino
Acids Analysis method for physiological samples
that utilizes temperature and eluant gradients.
METHOD
Analytical Conditions
COLUMN:
High-efficiency Lithium cation-exchange
column,
4.6 x 75 mm, 5 µm
Catalog number 0354675T
FLOW RATE:
0.55
mL/min
MOBILE PHASE:
1700-1125, Li365, Li375, RG003
Post-Column Conditions
POST-COLUMN SYSTEM:
Pinnacle PCX
Reactor Volume:
0.5
mL
TEMPERATURE:
130
˚C
REAGENT:
Trione
FLOW RATE:
0.5
mL/min
DETECTION:
UV/VIS 570 nm for primary amino acids
440 nm for secondary amino acids
Runtime:
72
min
Equilibration Time:
15
min
COLUMN OVEN
PROGRAM
TIME
TEMP
ºC
0
34
6
34
17
65
25
70
70
70
71
34
HPLC PROGRAM
TIME
1700-1125 %
Li365 %
Li375 %
RG003 %
0
100
0
0
0
10
100
0
0
0
19
40
60
0
0
32
0
100
0
0
43
0
100
0
0
43.1
0
0
100
0
57
0
0
100
0
57.1
0
0
70
30
72
0
0
70
30
72.1
100
0
0
0
1
Phosphoserine
10
Glutamic acid
19
Valine
28
b-Amino-i-butyric acid
37
1-Methyl-histidine
2
Taurine
11
Glutamine
20
Cystine
29
Homocystine
38
Histidine
3
Phosphoeth-anolamine
12
Sarcosine
21
Methionine
30
g-Amino-butyric acid
39
3-Methyl-histidine
4
Urea
13
a-Aminoadipic acid
22
Cystathionine
31
Tryptophan
40
Anserine
5
Aspartic acid
14
Proline
23
Isoleucine
32
Ethanolamine
41
Carnosine
6
Hydroxyproline
15
Glycine
24
Leucine
33
Ammonia
42
Arginine
7
Threonine
16
Alanine
25
Tyrosine
34
Hydroxylysines
43
Glucosaminic Acid*
8
Serine
17
Citrulline
26
Phenylalanine
35
Ornitine
9
Asparagine
18
a-Amino-n-butyric acid
27
b-Alanine
36
Lysine
*Internal Standard
70-Minute
Amino Acid Analysis
CATALOG INFORMATION
CATALOG
NO.
DESCRIPTION
0354675T
High-efficiency Lithium cation-exchange
column, 4.6 x 75 um
