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Carbamate Pesticide Analysis Columns
CARBAMATE PESTICIDE COLUMNS
 
Catalog # Description
0840250 Carbamate Column, expanded resolution,C8 4.0 x 250 mm & 1700-0063 Carbamate Test Mixture, 2.5 µg/mL, 1.5 mL
O846250 Carbamate Column, high resolution/capacity, C8 , 4.6 x 250 mm & 1700-0063 Carbamate Test Mixture, 2.5 µg/mL, 1.5 mL
1846150 Carbamate Column, rapid analysis, C18 , 4.6 x 150 mm & 1700-0063 Carbamate Test Mixture, 2.5 µg/mL, 1.5 mL

Pickering Laboratories carbamate columns are guaranteed to produce the separation of carbamate residues, specified by EPA 531.1, EPA 531.2 and AOAC 985.23 methods.

    

Post-column conditions for pesticide analysis:

Reagent 1: Hydrolysis reagent CB130 or CB130.2

Reagent 2: 100 mg of OPA, 2 g Thiofluor in 950 mL of CB910

Reactor 1: 100 C, 0.5 mL

Reactor 2: ambient. 0.1 mL

Reagents flow rate: 0.3 mL/min

Detection:

Fluorometer: λex 330 nm, λem 465 nm

The recommended gradient conditions are subject to change without notice. This may happen because of lot specific changes in the columns, or improvements in the overall method.

The recommended gradient for the column will always be included in the column package, and it supersedes the information in this catalog.

 

HIGH RESOLUTION / CAPACITY Carbamate column 0846250 (4.6 x 250 mm), C8, 5 μm

 

1  Aldicarb sulfoxide
2  Aldicarb sulfone
3  Oxamyl
4  Methomyl
5  3-Hydroxycarbofuran
6  Aldicarb
7  Propoxur
8  Carbofuran
  9  Carbaryl
10  1-Naphthol
11  Methiocarb
12  BDMC (internal standard)

CONDITIONS FOR AQUEOUS SAMPLES

Time (Min)

WATER %

MeOH %

100

0

1

100

0

1.1

82

18

36

30

70

39

30

70

39.1 0 100
41 0 100
41.1 100 0
55 100 0

FLOW RATE: 1 mL/min,
COLUMN TEMP.: 42 C, INJECTION VOLUME: Up to 400 L

   

 

CONDITIONS FOR METHANOLIC SAMPLES

Time (Min)

WATER %

MeOH %

85

15

1

85

15

44

25

75

44.1

0

100

49

0

100

49.1 85 15
57 85 15

FLOW RATE: 1 mL/min,
COLUMN TEMP.: 42 C, INJECTION VOLUME: 10 L

 

 

 

 
 

    

RAPID ANALYSIS Carbamate column 1846150 (4.6 x 150 mm), C18, 5 µm

CONDITIONS FOR AQUEOUS SAMPLES

Time (Min)

WATER %

MeOH %

100

0

1

100

0

1.1

88

12

26

30

70

26.1

0

100

28 0 100
28.1 100 0
38 100 0

FLOW RATE: 1 mL/min, COLUMN TEMP.: 42 C, INJECTION VOLUME: Up to 250 L

   

CONDITIONS FOR METHANOLIC SAMPLES

Time (Min)

WATER %

MeOH %

85

15

0.5

85

15

28.5

30

70

28.6

0

100

33.5

0

100

33.6 85 15
41 85 15

FLOW RATE: 1 mL/min,
COLUMN TEMP.: 42 C,
INJECTION VOLUME: 10 L

1  Aldicarb sulfoxide
2  Aldicarb sulfone
3  Oxamyl
4  Methomyl
5  3-Hydroxycarbofuran
6  Aldicarb
7  Propoxur
8  Carbofuran
  9  Carbaryl
10  1-Naphthol
11  Methiocarb
12  BDMC (internal standard)

   

EXPANDED RESOLUTION Carbamate column 0840250 (4.6 x 150 mm), C8, 5 µm

CONDITIONS FOR WATER/METHANOL GRADIENT

Time (Min)

WATER %

MeOH %

85

15

2

85

15

42

30

70

46

30

70

46.1

0

100

51 0 100
51.1 85 15
59 85 15

FLOW RATE: 0.8 mL/min,
COLUMN TEMP.: 50 C,
INJECTION VOLUME: 10 L for methanolic and up to 400 L for aqueous samples

   

CONDITIONS FOR WATER / ACETONITRILE GRADIENT 

Time (Min)

WATER %

ACN %

90

10

2

90

10

46

49

51

46.1

30

70

49 30 70
49.1 90 10
59 90 10

FLOW RATE: 0.8 mL/min,
COLUMN TEMP.: 50 C,
INJECTION VOLUME: 10 L for methanolic and up to 400 L for aqueous samples

1  Aldicarb sulfoxide
2  Aldicarb sulfone
3  Oxamyl
4  Methomyl
5  3-Hydroxycarbofuran
6  Aldicarb
7  Propoxur
8  Carbofuran
  9  Carbaryl
10  1-Naphthol
11  Methiocarb
12  BDMC (internal standard)

   

separation of 23 carbamates using 0840250 column (4.0 x 250 mm)

CONDITIONS FOR WATER/METHANOL GRADIENT 

Time (Min)

WATER %

MeOH %

85

15

2

85

15

42

30

70

46

30

70

46.1

0

100

51 0 100
51.1 85 15
59 85 15

FLOW RATE: 0.8 mL/min,
COLUMN TEMP.: 50 C,
INJECTION VOLUME: 10 L for methanolic and up to 400 L for aqueous samples

   

1 Aldicarb sulfoxide
2 Aldicarb sulfone
3 Oxamyl
4 Methomyl
5 3-Hydroxy
6 Aldicarb
7 Propoxur
8 Carbofuran
   9 Carbaryl
10 Methiocarb
11 BDMC internal standard
12 Butocarboxim sulfoxide
13 Butocarboxim sulfone
14 Ethiofencarb sulfoxide
15 Ethiofencarb sulfone
16 Butocarboxim
17 Ethiofencarb
18 Thiofanox sulfoxide
19 Thiofanox sulfone
20 Methiocarb sulfoxide
21 Methiocarb sulfone
22 3-Ketocarbofuran
23 Thiofanox
  N 1-Naphthol