Pickering Laboratories carbamate columns are guaranteed to produce the separation of carbamate residues, specified by EPA and AOAC methods. For C₁₈ columns, two water/methanol gradient conditions are available for analysis of methanolic and water samples. Expanded resolution C₈ column is capable of separating as many as 23 carbamates with either water/methanol or water/acetonitrile gradients. The difference in selectivity between Methanol and Acetonitrile protocols could be used for confirming peak identification.

Post-column conditions for pesticide analysis:

Reagent 1: Hydrolysis reagent CB130 or CB130.2

Reagent 2: 100 mg of OPA, 2 g Thiofluor™ in 950 mL of CB910

Reactor 1: 100 °C, 0.5 mL

Reactor 2: ambient. 0.1 mL

Reagents flow rate: 0.3 mL/min

Detection:

Fluorometer: λex 330 nm, λem 465 nm

The recommended gradient conditions are subject to change without notice. This may happen because of lot changes in the columns, or improvements in the overall method.

The recommended gradient for the column will always be included in the column package, and it supersedes the information in this catalog.

 

CONDITIONS FOR AQUEOUS SAMPLES Time (Min) WATER % MeOH % 0  100 0 1 100 0 1.1 82 18 36 30 70 39 30 70 39.1 0 100 41 0 100 41.1 100 0 55 100 0 FLOW RATE: 1 mL/min, COLUMN TEMP.: 42 ºC, INJECTION VOLUME: Up to 400 µL

CONDITIONS FOR METHANOLIC SAMPLES Time (Min) WATER % MeOH % 0  85 15 1 85 15 44 25 75 44.1 0 100 49 0 100 49.1 85 15 57 85 15 FLOW RATE: 1 mL/min, COLUMN TEMP.: 42 ºC, INJECTION VOLUME: 10 µL

CONDITIONS FOR AQUEOUS SAMPLES Time (Min) WATER % MeOH % 0  100 0 1 100 0 1.1 88 12 26 30 70 26.1 0 100 28 0 100 28.1 100 0 38 100 0 FLOW RATE: 1 mL/min, COLUMN TEMP.: 42 ºC, INJECTION VOLUME: Up to 200 µL

CONDITIONS FOR METHANOLIC SAMPLES Time (Min) WATER % MeOH % 0  85 15 0.5 85 15 28.5 30 70 28.6 0 100 33.5 0 100 33.6 85 15 41 85 15 FLOW RATE: 1 mL/min, COLUMN TEMP.: 42 ºC, INJECTION VOLUME: 10 µL

CONDITIONS FOR WATER/METHANOL GRADIENT Time (Min) WATER % MeOH % 0  85 15 2 85 15 42 30 70 46 30 70 46.1 0 100 51 0 100 51.1 85 15 59 85 15 FLOW RATE: 0.8 mL/min, COLUMN TEMP.: 50 ºC, INJECTION VOLUME: 10 µL for methanolic and up to 400 µL for aqueous samples

CONDITIONS FOR WATER / ACETONITRILE GRADIENT  Time (Min) WATER % ACN % 0  90 10 2 90 10 46 49 51 46.1 30 70 49 30 70 49.1 90 10 59 90 10 FLOW RATE: 0.8 mL/min, COLUMN TEMP.: 50 ºC, INJECTION VOLUME: 10 µL for methanolic and up to 400 µL for aqueous samples

CONDITIONS FOR WATER/METHANOL GRADIENT  Time (Min) WATER % MeOH % 0  85 15 2 85 15 42 30 70 46 30 70 46.1 0 100 51 0 100 51.1 85 15 59 85 15 FLOW RATE: 0.8 mL/min, COLUMN TEMP.: 50 ºC, INJECTION VOLUME: 10 µL for methanolic and up to 400 µL for aqueous samples