Pickering Laboratories Carbamate Pesticide Analysis Columns

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CARBAMATE PESTICIDE ANALYSIS COLUMNS

Pickering Laboratories Carbamate columns are guaranteed to produce the separation of carbamate residues, specified by EPA and AOAC methods. For C₁₈ columns, two water/methanol gradient conditions are available for analysis of methanolic and water samples. Expanded resolution C₈ column is capable of separating as many as 23 carbamates with either water/methanol or water/acetonitrile gradients. The difference in selectivity between Methanol and Acetonitrile protocols could be used for confirming peak identification.

Post-column conditions for pesticide analysis:

Reagent 1: Hydrolysis reagent CB130

Reagent 2: 100 mg of OPA, 2 g Thiofluor™ in 950 mL of CB910

Reactor 1: 100 °C, 0.5 mL

Reactor 2: ambient. 0.1 mL

Reagents flow rate: 0.3 mL/min

Detection:

Fluorometer: _ex 330 nm, _em 465 nm

The recommended gradient conditions are subject to change without notice. This may happen because of lot changes in the columns, or improvements in the overall method. The recommended gradient for the column will always be in the column package, and it supercedes the information in this catalog.

CARBAMATE COLUMN 1846250 (4.6 x 250 mm), C₁₈, 5 µm

1	Aldicarb sulfoxide
2	Aldicarb sulfone
3	Oxamyl
4	Methomyl
5	3-Hydroxycarbofuran
6	Aldicarb
7	Propoxur
8	Carbofuran
9	Carbaryl
10	1-Naphthol
11	Methiocarb
12	BDMC (internal standard)

CONDITIONS FOR AQUEOUS SAMPLES

TIME	WATER	MeOH %
0	100	0
1	100	0
1.1	82	18
36	30	70
39	30	70
39.1	0	100
41	0	100
41.1	100	0
55	100	0

Flow rate: 1 mL/min, column temperature 42 °C,
injection volume up to 400 µL

1	Aldicarb sulfoxide
2	Aldicarb sulfone
3	Oxamyl
4	Methomyl
5	3-Hydroxycarbofuran
6	Aldicarb
7	Propoxur
8	Carbofuran
9	Carbaryl
10	1-Naphthol
11	Methiocarb
12	BDMC (internal standard)

CONDITIONS FOR METHANOLIC SAMPLES:

TIME	WATER	MeOH %
0	85	15
1	85	15
44	25	75
44.1	0	100
49	0	100
49.1	85	15
57	85	15

Flow rate: 1 mL/min, column temperature 42 °C,
injection volume 10 µL

CARBAMATE COLUMN 1846150 (4.6 x 150 mm), C₁₈ , 5 µm

1	Aldicarb sulfoxide
2	Aldicarb sulfone
3	Oxamyl
4	Methomyl
5	3-Hydroxycarbofuran
6	Aldicarb
7	Propoxur
8	Carbofuran
9	Carbaryl
10	1-Naphthol
11	Methiocarb
12	BDMC (internal standard)

Conditions for Aqueous Samples:

TIME	WATER	MeOH %
0	100	0
1	100	0
1.1	88	12
26	30	70
26.1	0	100
28	0	100
28.1	100	0
38	100	0

Flow rate: 1 mL/min, column temperature 42 °C,
injection volume up to 250 µL

1	Aldicarb sulfoxide
2	Aldicarb sulfone
3	Oxamyl
4	Methomyl
5	3-Hydroxycarbofuran
6	Aldicarb
7	Propoxur
8	Carbofuran
9	Carbaryl
10	1-Naphthol
11	Methiocarb
12	BDMC (internal standard)

CONDITIONS FOR METHANOLIC SAMPLES

TIME	WATER	MeOH %
0	85	15
0.5	85	15
28.5	30	70
28.6	0	100
33.5	0	100
33.6	85	15
41	85	15

Flow rate: 1 mL/min, column temperature 42 °C,
injection volume 10 µL

CARBAMATE COLUMN 0840250 (4.0 x 250 mm), C8, 5 µm,
expanded resolution for 23+ compounds

1	Aldicarb sulfoxide
2	Aldicarb sulfone
3	Oxamyl
4	Methomyl
5	3-Hydroxycarbofuran
6	Aldicarb
7	Propoxur
8	Carbofuran
9	Carbaryl
10	1-Naphthol
11	Methiocarb
12	BDMC (internal standard)

CONDITIONS FOR WATER/METHANOL GRADIENT

TIME	WATER	MeOH %
0	85	15
2	85	15
42	30	70
46	30	70
46.1	0	100
51	0	100
51.1	85	15
59	85	15

Flow rate: 0.8 mL/min, column temperature 37 °C,
injection volume 10 µL for methanolic and
up to 400 µl for aqueous samples

1	Aldicarb sulfoxide
2	Aldicarb sulfone
3	Oxamyl
4	Methomyl
5	3-Hydroxycarbofuran
6	Aldicarb
7	Propoxur
8	Carbofuran
9	Carbaryl
10	1-Naphthol
11	Methiocarb
12	BDMC (internal standard)

CONDITIONS FOR WATER/METHANOL GRADIENT

TIME	WATER	ACN %
0	90	10
2	90	10
46	49	51
46.1	30	70
49	30	70
49.1	90	10
59	90	10

Flow rate: 0.8 mL/min, column temperature 37 °C,
injection volume 10 µL for methanolic and
up to 400 µL for aqueous samples

SEPARATION OF 23 CARBAMATES USING 0846250 COLUMN (4.6 x 250 mm)

1	Aldicarb sulfoxide
2	Aldicarb sulfone
3	Oxamyl
4	Methomyl
5	3-Hydroxycarbofuran
6	Aldicarb
7	Propoxur
8	Carbofuran
9	Carbaryl
10	Methiocarb
11	BDMC (internal standard)
12	Butocarboxim sulfoxide
13	Butocarboxim sulfone
14	Ethiofencarb sulfoxide
15	Ethiofencarb sulfone
16	Butocarboxim
17	Ethiofencarb
18	Thiofanox sulfoxide
19	Thiofanox sulfone
20	Methiocarb sulfoxide
21	Methiocarb sulfone
22	3-Ketocarbofuran
23	Thiofanox
N	1-Naphthol

CONDITIONS FOR WATER/METHANOL GRADIENT

TIME	WATER	MeOH %
0	85	15
2	85	15
42	30	70
46	30	70
46.1	0	100
51	0	100
51.1	85	15
59	85	15

Flow rate: 0.8 mL/min, column temperature 37 °C,
injection volume 10 µL for methanolic and
up to 400 µl for aqueous samples

CARBAMATE COLUMNS CATALOG INFORMATION

CATALOG NO.	DESCRIPTION
0840250	Carbamate Column, C₈, expanded resolution, 4.0 × 250 mm & 1700-0063 Carbamate Test Mixture, 2.5 µg/mL, 1.5 mL
1846250	Carbamate Column, C₁₈, high resolution/capacity, 4.6 × 250 mm & 1700-0063 Carbamate Test Mixture, 2.5 µg/mL, 1.5 mL
1846150	Carbamate Column, C₁₈, rapid analysis, 4.6 × 150 mm & 1700-0063 Carbamate Test Mixture, 2.5 µg/mL, 1.5 mL
18ECG001	Guard Cartridge Holder with 3 guard cartridges
18ECG002	Guard Cartridges, 2/pk