Pickering Laboratories Carbamate Pesticide Analysis Micro Columns

CARBAMATE PESTICIDE ANALYSIS MICRO COLUMNS

In order to conserve solvents and reagents, increase sensitivity, and to provide for LC-MS confirmation we developed micro columns (2.1 × 100, 150 mm) for carbamate pesticide analysis. The 5 µm packing material (C₁₈, C₈) was bonded in the same fashion as our standard columns to assure the correct selectivity and ruggedness. In support of the standard methods (US EPA 531.1 and AOAC 985.32), the special post-column derivatization system was engineered to scale thereby providing the micro post-column reactor. Since our micro columns provide superior separation, operate at low flow rate with volatile mobile phases, and have low-bleed packing material they are ideal for LC-MS analysis of carbamates.

Post-column conditions for pesticide analysis using micro columns:

Reagent 1: Hydrolysis reagent CB130

Reagent 2: 100 mg of OPA, 2 g Thiofluor™ in 950 mL of CB910

Reactor 1: 100 °C, 100 µL

Reactor 2: ambient, 20 µL

Reagents flow rate: 60 µL/min

Detection:

Fluorometer: _ex 330 nm, _em 465 nm

The recommended gradient conditions are subject to change without notice. This may happen because of lot changes in the columns, or improvements in the overall method. The recommended gradient for the column will always be in the column package, and it supercedes the information in this catalog.

MICRO CARBAMATE COLUMN 1821100 (2.1 x 100 mm), C₁₈, 5 µm

1	Aldicarb sulfoxide
2	Aldicarb sulfone
3	Oxamyl
4	Methomyl
5	3-Hydroxycarbofuran
6	Aldicarb
7	Propoxur
8	Carbofuran
9	Carbaryl
10	1-Naphthol
11	Methiocarb
12	BDMC (internal standard)

CONDITIONS FOR METHANOLIC SAMPLES

TIME	WATER	MeOH %
0	100	0
0.1	88	12
15	60	40
20	55.5	44.5
27	20	80
27.1	0	100
33	0	100
33.1	100	0
45	100	0

Flow rate: 0.2 mL/min, column temperature 42 °C,
injection volume 3 µL

CONDITIONS FOR AQUEOUS SAMPLES

TIME	WATER	MeOH %
0	100	0
1	100	0
1.1	88	12
18	60	40
23	55.5	44.5
30	20	80
30.1	0	100
35	0	100
35.1	100	0
48	100	0

Flow rate: 0.2 mL/min, column temperature 42 °C,
injection volume up to 150 µL

MICRO CARBAMATE COLUMN 0821150 (2.1 x 150 mm), C₈, 5 µm

1	Aldicarb sulfoxide
2	Aldicarb sulfone
3	Oxamyl
4	Methomyl
5	3-Hydroxycarbofuran
6	Aldicarb
7	Propoxur
8	Carbofuran
9	Carbaryl
10	Methiocarb
11	BDMC (internal standard)
12	Butocarboxim sulfoxide
13	Butocarboxim sulfone
14	Ethiofencarb sulfoxide
15	Ethiofencarb sulfone
16	Butocarboxim
17	Ethiofencarb
18	Thiofanox sulfoxide
19	Thiofanox sulfone
20	Methiocarb sulfoxide
21	Methiocarb sulfone
22	3-Ketocarbofuran
23	Thiofanox
N	1-Naphthol

CONDITIONS FOR METHANOLIC SAMPLES

TIME	WATER	MeOH %
0	100	0
0.1	90	10
23	56	44
30	56.5	44.5
40	25	75
41	0	100
45	0	100
45.1	100	0
58	100	0

Flow rate: 0.17 mL/min, column temperature 42 °C,
injection volume 3 µL

CARBAMATE MICRO COLUMNS CATALOG INFORMATION

CATALOG NO.	DESCRIPTION
0821150	Micro Analytical Column, C₈, 2.1 × 150 mm & 1700-0063 Carbamate Test Mixture, 2.5 µg/mL, 1.5 mL
1821100	Micro Analytical Column, C₁₈, 2.1 × 100 mm & 1700-0063 Carbamate Test Mixture, 2.5 µg/mL, 1.5 mL
1700-0204	Guard Cartridge Holder with 3 guard cartridges, for use with Micro columns
1700-0205	Guard Cartridges, 2/pk, for use with Micro columns