Pickering Laboratories
cation-exchange Glyphosate column is designed and
tested for analysis of Glyphosate and its primary
metabolite Aminomethylphosphonic acid (AMPA). This
short, isocratic method provides separation of the
peaks of interest. Reproducible performance is
guaranteed run to run and column to column.
Post-column
conditions for Glyphosate analysis:
Reagent 1: Oxidizing reagent—100 µL of 5%
Sodium Hypochlorite in 950 mL of GA116
Reagent 2: 100 mg of OPA, 2 g Thiofluor™ in
950 mL of GA104
Reactor 1: 36 °C, 0.5 mL
Reactor 2: ambient. 0.1 mL
Reagents flow rate: 0.3 mL/min
Detection:
Fluorometer: ex
330 nm, em 465
nm
GLYPHOSATE COLUMN
1954150 (4.0 x 150 mm) K+
CATION-EXCHANGE,
GUARD COLUMN (3.0 x 20 mm), K+
CATION EXCHANGE
CONDITIONS
TIME
K200 %
RG019 %
0
100
0
15
100
0
15.1
0
100
17
0
100
17.1
100
0
27
100
0
Flow rate: 0.4
mL/min, column temperature 55 °C,
injection volume 10 µL
GLYPHOSATE COLUMN
CATALOG INFORMATION
CATALOG
NO.
DESCRIPTION
1954150
Cation-exchange Column for Glyphosate
analysis, 4 × 150 mm & 1700-0080 Glyphosate
test mixture, 2.5 μg/mL, 1.5 mL
1953020
Cation-exchange Guard Column, 3.0 × 20 mm
1952150
Micro
Cation-exchange Column, K+ form, 2.1 × 150 mm
& 1700-0080 Glyphosate test mixture, 2.5 µg/mL,
1.5 mL
1954250
Column,
Glyphosate 4 x 250 mm & 1700-0080 Glyphosate
test mixture,
2.5 µg/mL, 1.5 mL
ex
330 nm, 
