PARALYTIC SHELLFISH TOXINS ANALYSIS COLUMN

LC Condition:
Flow rate: 0.8 mL/min, column temperature 40 °C

Post-column conditions:
Reagent 1: Periodic acid (7 mM) in 50 mM Potassium Phosphate buffer, pH 9.0
Reactor 1: 75 °C, 2.0 mL
Reagent 2: 0.5 M Acetic Acid
Reactor 2: ambient, 0.1 mL
Reagents flow rate: 0.4 mL/min

Detection:
Fluorometer: _ex 330 nm, _em 390 nm

Mobile Phases: A. For C1–C4 toxins: Tetrabutylanmmonium phosphate (1 mM) adjusted to pH 5.8 with acetic acid B. For GTX-1 to GTX-6, dcGTX2, and dcGTX3: Sodium 1-heptanesulfonate (2 mM) in 10 mM Ammonium phosphate, pH 7.1 C. For STX, neoSTX, and dcSTX: Sodium 1-heptanesulfonate (2 mM) in 10 mM Ammonium phosphate, pH 7.1-Acetonitrile (100 +5)

PARALYTIC SHELLFISH TOXINS CATALOG INFORMATION

CATALOG NO. DESCRIPTION 0846150 Reversed-phase column for analysis of paralytic shellfish toxins, C8, 4.6 × 150 mm 18ECG001 Guard Cartridge Holder with 3 cartridges 18ECG002 Guard Cartridges, 2/pk.