SODIUM AMINO ACID ANALYSIS COLUMNS

Ion-exchange chromatography followed by post-column derivatization has been a method of choice for amino acid analysis for many years. Pickering ion-exchange columns allow you to obtain consistent results with sensitivity, stability, selectivity and speed. Sodium columns and buffers systems are designed for amino acids analysis of hydrolyzed samples.

Post-column conditions for amino acids analysis:

Reagent: TRIONE®

Reactor: 130 °C, 0.5 mL

Reagent flow rate: 0.3 mL/min

Detection:

UV-Vis detector: =570 nm for primary amino acids, =440 nm for secondary amino acids

or

Reagent: 300 mg of OPA, 2 g Thiofluor™, 3 mL of 30% Brij® 35 solution in 950 mL of OD104

Reactor: 45 °C, 0.15 mL

Reagent flow rate: 0.3 mL/min

Detection:

Fluorometer: _ex 330 nm, _em 465 nm

The recommended gradient conditions are subject to change without notice. This may happen because of lot changes in the columns, or improvements in the overall method. The recommended gradient for the column will always be in the column package, and it supersedes the information in this catalog.

		1 Methionine-D,L,- Sulfoxide 2 trans-4-Hydroxy-LProline 3 Aspartic Acid 4 Threonine 5 Serine 6 Glutamic Acid 7 Proline 8 Glycine 9 Alanine 10 Cystine 11 Valine 12 Methionine 13 Isoleucine 14 Leucine 15 Tyrosine 16 Phenylalanine 17 D,L & allo-Hydroxylysine 18 Lysine 19 Ammonia 20 Histidine 21 Tryptophan 22 Arginine
CONDITIONS TIME 1700-0112 % Na740 % RG011 %   0 100   0   0     12 100   0   0     34 0   100   0     53 0   100   0     53.1 0   0   100     55 0   0   100     55.1 100   0   0     67 100   0   0   Flow rate: 0.4 mL/min, column temperature 48 °C, injection volume: 10 µL of 0.25 µmole/mL Std.