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AFLATOXIN & Ochratoxin
SAMPLE PREPARATION COLUMNS
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SIMULTANEOUS ANALYSIS OF
AFLATOXINS AND OCHRATOXIN A - SAMPLE PREPARATION
Aflatoxins and Ochratoxin A
are produced by fungi, e.g. Aspergillus and
Penicillium species. Therefore both toxin types
are found together in many foods and animal feeds,
e.g. cereals. Thus it is appropriate to analyze
one extract for the occurrence of both mycotoxins
in a single step.
A significant assistance is
the cleanup of extracts by a combined
immunoaffinity column for both mycotoxin types in
one step. The subsequent analysis may then be
performed by HPLC with post-column derivatization
or LC-MS.
Pickering Laboratories’
combination sample preparation column
Afla-OtaClean from LCTech provides an ideal
immunoaffinity column. This combination column is
very tolerant towards many matrices and allows for
a comprehensive cleanup of the aflatoxins B1, B2,
G1 and G2 as well as Ochratoxin A. The high
maximum capacity of 150 ng for Aflatoxin B1 and
Ochratoxin A provides a wide a measurement range.
|
MATRIX
|
B1 |
B2 |
G1 |
G2 |
OTA |
|
Maize (Afla 10 ppb, OTA 14.3 ppb) |
107 % |
91 % |
103 % |
75 % |
97 % |
|
Rice (Afla 10 ppb, OTA 14.3 ppb) |
107 % |
93 % |
98 % |
85 % |
101 % |
|
Malt (Afla 10 ppb, OTA 14.3 ppb) |
98 %
|
99 % |
97 % |
70 % |
96 % |
|
Raisins (Afla 10 ppb, OTA 14.3 ppb) |
99 % |
106 % |
101 % |
69 % |
97 % |
|
Catalog # |
Description |
|
1705-0005
|
Alfa-OtaClean sample prep column, 25/pkg |
SAMPLE
PREPARATION COLUMN FOR AFLATOXIN ANALYSIS
The new Pickering
Laboratory immunoaffinity column AflaCLEAN from
LCTech was developed for the sample preparation of
foods for the aflatoxin analysis using HPLC. With
a maximum loading capacity of 100 ng Aflatoxin B1
and a selectivity against Aflatoxins B1, B2, G1
and G2. AflaCLEAN columns are available in the
practical 3 mL polypropylene format.
|
MATRIX |
B1 |
B2 |
B3 |
B4 |
|
Chili |
116 % |
117 % |
121 % |
92 % |
|
Dates |
108 % |
96 % |
116 % |
72 % |
|
Peanut Butter |
95 % |
98 % |
93 % |
84 % |
|
Peanuts |
114 % |
113 % |
102 % |
91 % |
|
Figs |
115 % |
104 % |
110 % |
74 % |
|
Hazelnuts |
98 % |
100 % |
95 % |
83 % |
|
Maize |
101 % |
98 % |
103 % |
80 % |
|
Almonds |
91 % |
91 % |
90 % |
79 % |
|
Pistachios |
90 %
|
90 % |
100 % |
75 % |
|
Rice |
103 % |
104 % |
104 % |
89 % |
|
Cinnamon |
87 % |
85 % |
86 % |
87 % |
|
Catalog # |
Description |
|
1705-0003 |
AlfaClean sample prep column,
25/pkg |
SAMPLE
PREPARATION COLUMN FOR Ochratoxin ANALYSIS
The new Pickering
Laboratory immunoaffinity column OtaCLEAN was
developed for sample preparation of food for the
Ochratoxin A analysis using HPLC. The antibody
employed possesses a very high Ochratoxin A
specificity. This leads to exceptional
chromatographic results without any interfering
secondary signals and very high recovery rates.
Independent of the complexity of the matrix,
excellent results can be achieved.
|
MATRIX |
RECOVERY RATE |
|
Beer
|
96 % |
|
Cocoa
|
92 % |
|
Coffee
|
100 % |
|
Coffee Decaf
|
91 % |
|
Duran Wheat
|
92 % |
|
Grapes
|
91 % |
|
Horse Feed
|
94 % |
|
Instant Coffee
|
97 % |
|
Liquorices
|
99 % |
|
Malt
|
104 % |
|
Nut Cream
|
91 % |
|
Paprika
|
92 % |
|
Pig feed
|
88 % |
|
Raisins
|
96 % |
|
Roast Coffee
|
101 % |
|
Rice
|
90 % |
|
Red Wine
|
108 % |
|
Rye Bran
|
91 % |
|
Wort
|
107 % |
|
|
|
|
Catalog # |
Description |
|
1705-0004 |
OtaClean sample prep column, 25/pkg |
NOTE: The column is available in
the practical 3 mL polypropylene format
GLYPHOSATE AND
AMPA SAMPLE CLEANUP CARTRIDGES
Pickering SPE
sample cleanup cartridges are used to treat
samples prior to injection. The strong cation
exchange resin is composed of sulfonic acid
functional groups attached to a styrene
divinylbenzene copolymer lattice. The high
selectivity and ruggedness provides a simple and
universal method for removing matrix interference
from the sample. The SPE sample cleanup
cartridges are ideal for the clean up of
vegetables, fruits and crop samples in the
analysis of Glyphosate and AMPA.
|
MATRIX |
GLYPHOSATE |
AMPA |
|
Alfalfa |
129 % |
116 % |
|
Strawberry |
84 %
|
82 % |
|
Broccoli
|
97 %
|
95 % |
Sample Preparation
Extraction
25 g of homogenous sample add
enough water (after estimation of moisture
content) to make the total volume of water 125 mL.
Blend and Centrifuge.
Matrix Specific
Modification
• High water content – reduce
sample amount to 12.5 g
• High protein content – add
100 μL HCl to 20 mL of extract, shake and
centrifuge.
• High Fat content – do the
methylene chloride partition twice.
Clean Up
Methylene Chloride Partition
• To 20 mL of aqueous extract
add 15 mL methylene chloride. Shake for 2-3
min and centrifuge.
• To 4.5 mL of aqueous layer
add 0.5 mL acidic modifier solution. Shake
and centrifuge.
SPE Clean up
• Prepare SPE cartridge (refer
to product abstract PA210)
• Transfer 1 mL portions of
SPE mobile phase (refer to method abstract MA206
for formulation) and discard the effluent.
• Elute analytes with 12 mL
SPE mobile phase
Concentration
• Evaporate to dryness using
rotary evaporator or a vacuum vortex-type
evaporator or lyophilize
• Re-dissolve in 2 mL of the
SPE mobile phase
|
Catalog # |
Description |
|
1705-0001 |
SPE sample cleanup cartridges, 50/pkg |
|
1750-1118 |
Aminoglycoside Extraction Solution, 4x950 mL |
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