ALKION™ COLUMN

The stationary phase in the ALKION™ column is a rigid, non-porous polymeric phase that is surface sulfonated. The two modes of separation are ion-exchange and moderate partitioning. The low capacity of the ion-exchange resin makes it an ideal phase for the separation of strongly basic and positively charged compounds. The reversed-phase character allows for discrimination between closely related species.

The unique properties of this column allow using it for a wide range of applications.

Post-column conditions:

Reagent 1: 300 mg of OPA, 2g Thiofluor™, 3 mL of 30% Brij® 35 in 950 mL of OD104

Reactor: 45 °C, 0.15 mL

Reagent flow rate: 0.3 mL/min

Detection:

Fluorometer: _ex 330 nm, _em 465 nm

Post-column conditions:

Reagent 1: 300 mg of OPA, 2g Thiofluor™, 3 mL of 30% Brij® 35 in 950 mL of OD104
Reactor: 45 °C, 0.15 mL
Reagent flow rate: 0.3 mL/min

Detection:

Fluorometer: _ex 330 nm, _em 465 nm

1 Histamine 2 Putrescine 3 Cavaverine 4 Spermidine 5 Spermine
Conditions: TIME [min] K600 [%] K563 [%] K130 [%]   0 100   0   0     6 100   0   0     15 0   100   0     21 0   100   0     21.1 0   0   100     23 0   0   100     23.1 100   0   0     29 100   0   0   Flow rate: 0.8 mL/min, column temperature 40 °C, injection volume 10 µL

Post-column conditions:

Reagent 1: 300 mg/L of Sodium Dithionite in 0.3 M Sodium Hydroxide

Reactor: 35 °C, 0.15 mL

Reagent flow rate: 0.3 mL/min

Detection:

UV-Vis detector: =395 nm for PQ, =378 nm for DQ

PQ Paraquat DQ Diquat

Note: Above chromatogram is approximately 1 ppm in 200 µL.

Conditions: TIME 1700-1101 % 1700-1102 % 1700-1103 % i-PROPANOL %   0 31.5   53.5   0   15     10 28   47   10   15     15 28   47   10   15     15.1 0   85   10   5     17 0   85   10   5     17.1 31.5   53.5   0   15     23 31.5   53.5   0   15   Flow rate: 0.8 mL/min, column temperature 50 °C, injection volume: up to several µL

Post-column conditions:

Reagent 1: 300 mg of OPA, 2g Thiofluor™, 3 mL 30 % Brij® 35 in 950 mL of OD104

Reactor: 45 °C, 0.15 mL

Reagent flow rate: 0.3 mL/min

Detection:

Fluorometer: _ex 330 nm, _em 465 nm

Post-column conditions:

Reagent 1: 0.75 N Sodium Hydroxide

Reactor 1: ambient, 0.1 mL

Reagent 2: 0.6 % Ninhydrin

Reactor 2: 60 °C, 0.5 mL

Reagents flow rate: 0.3 mL/min

Fluorometer: _ex 395 nm, _em 500 nm

or

Reagent 1: Oxidizing Reagent: 100 µL of 5% Sodium Hypochlorite in GA116

Reactor 1: 50 °C, 0.5 mLC

Reagent 2: 300 mg of OPA, 2 g Thiofluor™, 3 mL of 30% Brij® 35 solution in 950 mL of OD104

Reactor 2: ambient, 0.1 mL

Detection:

Fluorometer: _ex 330 nm, _em 465 nm

Conditions: TIME 1700-1101 % 1700-1102 % 1700-1103 %   0 60   40   0     10 60   40   0     10.1 48   48   4     15 48   48   4     15.1 60   40   0     21 60   40   0   Flow rate: 0.8 mL/min, column temperature 40 °C, injection volume up to several µL

ALKION™ COLUMN CATALOG INFORMATION

CATALOG NO. DESCRIPTION 9410917 ALKION™ Cation-exchange column, K form, 4.0 × 150 mm 9493020 ALKION™ Guard column, K form, 3.0 × 20 mm