|
The stationary phase in the ALKION™ column is a
rigid, non-porous polymeric phase that is surface
sulfonated. The two modes of separation are
ion-exchange and moderate partitioning. The low
capacity of the ion-exchange resin makes it an
ideal phase for the separation of strongly basic
and positively charged compounds. The
reversed-phase character allows for discrimination
between closely related species.
The unique properties
of this column allow using it for a wide range of
applications.
Post-column conditions:
Reagent 1: 300 mg of OPA, 2g Thiofluor™, 3
mL of 30% Brij® 35 in 950 mL of OD104
Reactor: 45 °C, 0.15 mL
Reagent flow rate: 0.3 mL/min
Detection:
Fluorometer:  ex
330 nm, em 465
nm
|
 |
|
0 |
100 |
0 |
|
12 |
100 |
0 |
|
12.1 |
0 |
100 |
|
15 |
0 |
100 |
|
15.1 |
100 |
0 |
|
20 |
100 |
0 |
Flow rate: 0.8 mL/min, column
temperature 40 °C,
injection volume: 10 µL |
Post-column
conditions:
Reagent 1: 300 mg of OPA, 2g Thiofluor™, 3
mL of 30% Brij® 35 in 950 mL of OD104
Reactor: 45 °C, 0.15 mL
Reagent flow rate: 0.3 mL/min
Detection:
Fluorometer: ex
330 nm, em 465
nm
|
 |
|
1 |
Histamine |
|
2 |
Putrescine |
|
3 |
Cavaverine |
|
4 |
Spermidine |
|
5 |
Spermine |
|
|
 |
|
|
0 |
100 |
0 |
0 |
|
6 |
100 |
0 |
0 |
|
15 |
0 |
100 |
0 |
|
21 |
0 |
100 |
0 |
|
21.1 |
0 |
0 |
100 |
|
23 |
0 |
0 |
100 |
|
23.1 |
100 |
0 |
0 |
|
29 |
100 |
0 |
0 |
Flow rate: 0.8 mL/min, column
temperature 40 °C,
injection volume 10 µL |
Post-column
conditions:
Reagent 1: 300 mg/L of Sodium Dithionite in
0.3 M Sodium Hydroxide
Reactor: 35 °C, 0.15 mL
Reagent flow rate: 0.3 mL/min
Detection:
UV-Vis detector: =395 nm
for PQ, =378 nm for DQ
|
|
|
Note: Above
chromatogram is approximately 1 ppm in 200 µL.
|
|
|
|
Flow rate: 0.8 mL/min, column
temperature 50 °C,
injection volume: up to several µL |
Post-column conditions:
Reagent 1: 300 mg of OPA, 2g Thiofluor™, 3 mL 30 %
Brij® 35 in 950 mL of OD104
Reactor: 45 °C, 0.15 mL
Reagent flow rate: 0.3 mL/min
Detection:
Fluorometer: ex
330 nm, em 465
nm
|
0 |
|
|
|
|
5 |
|
|
|
|
15 |
|
|
|
|
20 |
|
|
|
|
20.1 |
|
|
|
|
21 |
|
|
|
|
21.1 |
|
|
|
|
28 |
|
|
|
Flow rate: 0.8 mL/min, column
temperature 40 °C,
injection volume 10 µL |
|
 |
|
Flow rate: 0.8 mL/min, column
temperature 40 °C,
injection volume 10 µL |
 |
|
Flow rate: 0.8 mL/min, column
temperature 40 °C,
injection volume 10 µL |
 |
|
| |
