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Method Abstract
for Post-column Liquid Chromatography 105.1
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The paralytic shellfish toxins
are a group of 18 secondary metabolites deposited
in bivalve mollusks by dinoflagelates.
Dinoflagelates blooms are seasonal, occuring
during warm months. Since it is
unpredictable whether an infestation will occur,
the shellfish population should be regularly
monitored for toxins. Ingestion of
contaminated shellfish can lead to paralytic
shellfish poisoning; a life-threatening illness.
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Analytical
Conditions |
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COLUMN: |
Reversed-phase column for
PST analysis,
C8, 4.6 x 150 mm, Catalog No. 0846150 |
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TEMPERATURE: |
40 °C |
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FLOW RATE: |
0.8 mL/min |
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MOBILE PHASE: |
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A. For C1–C4 toxins: |
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Tetrabutylanmmonium
phosphate (1 mM) adjusted to pH 5.8 with
acetic acid |
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B. For GTX-1 to
GTX-6, dcGTX2, and dcGTX3: |
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Sodium
1-heptanesulfonate (2 mM)
in 10 mM Ammonium phosphate, pH 7.1 |
| C. For
STX, neoSTX, and dcSTX: |
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Sodium
1-heptanesulfonate (2 mM) in 10 mM
Ammonium phosphate, pH 7.1 :
Acetonitrile (2 : 1) |
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Post-column Conditions |
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POST-COLUMN SYSTEM: |
Vector PCX or Piinnacle
PCX |
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REAGENT 1: |
Periodic acid (7 mM) in 50 mM
Potassium Phosphate buffer, pH 9.0 |
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REACTOR 1: |
75 °C, 2.0 mL |
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REAGENT 2: |
0.5 M Acetic Acid |
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REACTOR 2: |
ambient, 0.1 mL |
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FLOW RATE: |
0.4 mL/min each |
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DETECTION: |
Fluorometer
 ex:
330 nm
em:
390 nm |
REFERENCES
1. Y. Oshima, K Sugino, T. Yasumoto
“Latest Advances in HPLC Analysis of Paralytic
Shellfish
Toxins in Bioactiva Molecules” 10, (1989) 319–326
2. J. J. Sullivan, M.M. Wekell, and
L. L. Kentala,
J. Food Sci. 50(1) (1985) 26–29.
3. Y. Oshima, JOAC, INTL. 78(2) (1995)
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