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Method Abstract for Post-column Liquid
Chromatography 115
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Post-column derivatization of carbamates with
hydroxide and OPA is the method of choice for the
detection of carbamates because of its high
selectivity and high sensitivity (US EPA 531.1 &
AOAC 985.23). To confirm the finding of
carbamates in the tested samples, there are
various possibilitites:
To
confirm the finding of carbamates in the tested
samples, there are various possibilitites:
•
Alternate columns to give different
retention time or order of elution.
•
Fluorescence scan of the isoindole
end-products.
•
Mass-spectrum of the carbamates.
Options 1 & 2 are already available to many
Pickering customers. For option 3, the resolution
of the carbamates has always been an issue with
the necessary smaller ID column. The new 2.1 mm ID
Pickering Carbamates Column provides the
resolution. Because the new column contains the
same durable phase as our regular Carbamates
Column, it will have minimum leaching (critical
for the mass spectrometer).
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Analytical
Conditions |
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COLUMN: |
2.1 x 100 mm
Carbamates Column
Catalog No. 1821100 |
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TEMPERATURE: |
42 °C |
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FLOW RATE: |
0.2 mL/min |
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MOBILE PHASE: |
A=H2O; B=MeOH |
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Post-column Conditions |
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POST-COLUMN SYSTEM: |
Vector PCX or
Pinnacle PCX |
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REAGENT 1: |
CB 130 |
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REACTOR 1: |
100 µL, 100 °C |
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REAGENT 2: |
OPA, Thiofluor in CB
910 |
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REACTOR 2: |
20 µL, Ambient |
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FLOW RATE: |
60 µL/min |
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DETECTION: |
Fluorometer
 ex: 330
nm
em: 464
nm |
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0 |
100 |
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0.1 |
88 |
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15 |
60 |
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20 |
55.5 |
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27 |
20 |
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27.1 |
0 |
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33 |
0 |
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45.1 |
88 |
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