Pickering Laboratories Guaranteed Chemistry

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Method Abstract for Post-column Liquid Chromatography 117

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Paraquat and Diquat are widely used in agriculture and industry.  They are employed as contact herbicides, as harvest aids, for pasture renovation and for aquatic weed control. Their environmental persistence allows for contamination of water supplies; wells, lakes, rivers, etc. Since Paraquat is a Class I Toxin and Diquat is a Class II Toxin, water resources must be monitored for the presence of these herbicides.

This method employs ion exchange chromatography so filtration is the only sample preparation necessary. Also, since both Paraquat and Diquat have a fixed +2 charge, 2 mL can be injected without peak degradation. Post-column derivitization improves sensitivity and greatly improves selectivity by shifting the detection to longer wavelengths.

METHOD

Analytical Conditions

COLUMN:

ALKION™column 4 x 150mm, Catalog No. 9410917,
Guard column 3 x 20mm,
Catalog No. 9493020

TEMPERATURE:

50 °C

FLOW RATE:

0.8 mL/min

MOBILE PHASE:

K01, Potassium buffer
K02, Potassium titrant
K03, Potassium ionic strength adjuster

 
TIME
[min]
K01
[%]
K02
[%]
K03
[%]
IPA
[%]
  0
31.5  
53.5  
0  
15  
  10
28  
47  
10  
15  
  15
28  
47  
10  
15  
  15.1
0  
85  
10  
5  
  17
0  
85  
10  
5  
  17.1
31.5  
53.5  
0  
15  

Post-column Conditions

POST-COLUMN  SYSTEM:

Pinnacle PCX

REACTION DWELL:

0.15 mL

TEMPERATURE:

35 °C

REAGENT:

300 mg/L Sodium Dithionite in 0.3 M Sodium Hydroxide

FLOW RATE:

0.3 mL/min

DETECTION:

395 nm for Paraquat and 378 nm for Diquat

Without post-column derivatization: 257 nm for
Paraquat and 308 nm for Diquat

  

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