Nonnutritive sweeteners are
widely used in foods and beverages. Since some
studies raised questions about long-term safety of
these compounds, their concentration in food
products is regulated. Among the artificial
sweeteners used worldwide Saccharin and Cyclamate
cause most controversy and their use is restricted
in many countries, including the US. Liquid
chromatography is a method of choice for
artificial sweeteners but Cyclamate analysis is
complicated by the fact that this compound does
not exhibit noticeable UV absorbance.
This method allows for
simultaneous analysis of Acesulfame, Saccharin and
Cyclamate by LC with post-column derivatization
followed by fluorescence detection. Since the
sweeteners are commonly used in combination, a
single method of analysis is preferred.
METHOD
Analytical Conditions
ANALYTICAL COLUMN:
Reversed-phase column,
C18, 2x100 mm
TEMPERATURE:
42°C
FLOW RATE:
0.2 mL/min
MOBILE PHASE:
0.02 M Potassium
Phosphate Monobasic in water. Isocratic for 8
min, followed by washing the column with 70%
ACN – 30% 0.02 M KH2PO4
ex:
365 nm,
