Pickering Laboratories Guaranteed Chemistry

Quality Guaranteed Pickering Laboratories HPLC post-column instruments and solutions products catalog for environmental, pharmaceutical and biochemical laboratories.
     
 

 

 

Printer Friendly Version

 

 

Method Abstract for Post-column Liquid Chromatography 325

Here you can download this material in PDF format*

Formaldehyde is used for disinfections of industrially manufactured products. However, it is detrimental to health and its potential oncogenicity led to the current utilization of a formaldehyde donor for long-term preservation. Such donors are in hydrolytic equilibrium with formaldehyde producing free formaldehyde on exposure to moisture. This method is used to quantify the free formaldehyde without upsetting the existing equilibrium. Potential interferences are either separated from formaldehyde or they don’t react with the post-column reagent. The lutidine derivative is highly fluorescent resulting in ppm detection levels.

METHODS

Analytical Conditions

COLUMN:

Diol, 5µm, 4.6 x 250mm

TEMPERATURE:

40° C

FLOW RATE:

0.5mL/min

MOBILE PHASE:

Acetonitrile: Water (85:15) isocratic

Post-Column Conditions

 

POST-COLUMN SYSTEM:

Pinnacle PCX

REACTOR VOLUME:

0.5mL

TEMPERATURE:

100° C

REAGENT:

0.81 M Ammonimn acetate

0.12 M Galcial acetic acid (pH=5)

0.05 M 2, 4-Pentanedione with water

FLOW RATE:

0.4 mL/min

DETECTION:

Fluorometer

λEX = 412 nm

λEM = 510 nm

 

* Please use Acrobat Reader to open this file

 

 

Home  :  Products  :  Library  :  Service & Support  :  About Us  :  Contact Us  :  Distributor List  :  News & Events  :  Sitemap

1-800-654-3330  :  ©2007 Pickering Laboratories, Inc. -- All Rights Reserved