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Pickering Laboratories, Inc. 1280 Space Park Way, Mountain View, CA 94043 USA Phone: (800) 654-3330; (650) 694-6700 Email: Sales@pickeringlabs.com |
| Method Abstract for Post-column Liquid Chromatography 382 |
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Pickering Laboratories specializes in the manufacturing of cation-exchange columns and eluants for amino acid analysis. Post-column derivatization with Ninhydrin offers unmatched selectivity and reproducibility of the analysis for most challenging matrixes. Pinnacle PCX post-column derivatization system allows analysts to combine eluant gradient capabilities of modern HPLC instruments with column temperature gradients. We introduce new accelerated Amino Acids Analysis method for physiological samples that utilizes temperature and eluant gradients. Method Analytical Conditions Column: High-efficiency Lithium cation-exchange column, 4.6 x 75 mm, 5 μm Catalog number 0354675T Flow Rate: 0.55 mL/min Mobile Phase: 1700-1125, Li365, Li375, RG003 Post-column Conditions Post-column System: Pinnacle PCX Reactor Volume: 0.5 mL Temperature: 130 ˚C Reagent: Trione Reagent Flow Rate: 0.5 mL/min Detection: UV/VIS 570 nm for primary amino acids 440 nm for secondary amino acids Runtime: 72 min Equilibration Time: 15 min
*Internal Standard |
