For Post-column Analysis of Sulfa Drugs in
feed and animal tissues
p-Dimethylaminobenzaldehyde (DMAB;
Ehrlich’s Reagent) reacts rapidly with many
primary amines to form a complex with maximum
absorbance at 450 nm.
Reversed-phase LC followed by post-column
derivatization is used to determine the levels
of Sulfamethazine, Sulfathiazole and related
compounds in feeds, premixes and animal
tissues.
Although these compounds have a UV chromophore,
so do most of the other constituents in the
complex sample matrix. Forming a colored
derivative post-column allows shifting of the
detection wavelength into the visible range
thus greatly enhancing selectivity.
p-DIMETHYLAMINOBENZALDEHYDE (DMAB)
CATALOG INFORMATION
