For Automated Post-column Derivatization of
Primary amines form highly fluorescent adducts
when reacted with o-Phthalaldehyde (OPA) and a
mercaptan under basic conditions.
The products of this reaction,
exhibit optimal excitation at 330 nm and
maximal emission at 465 nm.
Pickering's OPA is specially prepared to meet
the demanding requirements of high-sensitivity
pre-and post-column HPLC derivatization
methods. Our repurification process actually
begins with commercially available 99 %
The entire process is controlled to eliminate
trace interfering contamination. Lot quality
is verified by post-column HPLC using a
high-sensitivity fluorescence detector.