Chromatographic Grade™

For Automated Post-column Derivatization of Primary Amines

Primary amines form highly fluorescent adducts when reacted with o-Phthalaldehyde (OPA) and a mercaptan under basic conditions.

The products of this reaction, 1-alkyl-2alkylthio-substituted isoindoles, exhibit optimal excitation at 330 nm and maximal emission at 465 nm.

Pickering's OPA is specially prepared to meet the demanding requirements of high-sensitivity pre-and post-column HPLC derivatization methods. Our repurification process actually begins with commercially available 99 % material.

The entire process is controlled to eliminate trace interfering contamination. Lot quality is verified by post-column HPLC using a high-sensitivity fluorescence detector.