Pickering Laboratories Trione® Ninhydrin Reagent

TRIONE® NINHYDRIN REAGENT

Chromatographic Grade™

A prepared Reagent for Automated Post-column Derivatization of Primary and Secondary Amines

TRIONE® Ninhydrin reagent is specially formulated for amino acid analysis. It contains Ninhydrin, Hydrindantin (reduced Ninhydrin), a Lithium Acetate buffer, and Sulfolane, a water-miscible organic solvent. The solvent is necessary to maintain the solubility of both the Hydrindantin and the primary amine product, Ruhemann’s Purple. The buffer is required because the reaction is pH dependent. The active ingredients—Ninhydrin and Hydrindantin are required for proper development of secondary and primary amines, respectively.

TRIONE® Ninhydrin reagent

TRIONE® is so stable that is does not require refrigeration, either in shipment, storage, or in the reservoir. Quantitation is consistent from the first to the last mL, so there is no waste. The high signal-to-noise ratio of TRIONE®, when compared to DMSO-containing reagents, permits detection sensitivity to be increased with minimum increase in background noise—a feature particularly appreciated at sample concentrations of <50 pico moles.

Two Preparations are available to suit your usage and storage requirements:

T100/T100C

Pour into your reservoir and use; the ultimate in convenience
with a minimum of handling
3-month* shelf life

T200

Combine two solutions, swirl, and use
12-month* shelf life before mixing preparation; one month in the reservoir

TRIONE® NINHYDRIN REAGENT CATALOG INFORMATION

CATALOG NO.	DESCRIPTION	QUANTITY
T100	TRIONE® Ninhydrin Reagent (3-month* shelf life)	each ( 950 mL/bottle)
T100C	TRIONE® Ninhydrin Reagent (3-month* shelf life)	case of 4 (950 mL/bottle)
T200	TRIONE® Two-part Ninhydrin Reagent (12-month* shelf life before mixing)	to prepare case of 4 (900 mL/bottle)

* From date of manufacture