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N,N-Dimethyl-2-mercaptoethylamine
hydrochloride
– Chromatographic Grade™
For Automated Post-column Derivatization of
Primary Amines
Primary amines form highly fluorescent adducts
when reacted with o-Phthalaldehyde (OPA)
and a mercaptan under basic conditions.
The products of this reaction,
1-alkyl-2-thioalkyl-subsitituted isoindoles,
exhibit optimal excitation at 330 nm and
maximal emission at 465 nm.
Pickering’s Thiofluor™, a solid, nearly
odorless nucleophile, is a superior substitute
for 2-Mercaptoethanol in the preparation of
OPA reagents. It forms a more stable reagent
and a longer-lasting fluorophore with OPA than
does 2-Mercaptoethanol, yet it has the same
fluorescence properties.
Unlike the volatile 2-Mercaptoethanol,
Thiofluor™ will not migrate through the gas
manifold and regulator of the OPA reagent
pressurization system.
Two grams of Thiofluor™ is equivalent to 1 mL
of 2-Mercaptoethanol. |
