Vanillin For Post-column Analysis of Polyether Antibiotics Here you can download this materials in PDF format*

INTRODUCTION Chromatographic Grade® for low noise and optimum sensitivity CO2-protected, 30 gram package Visible-range detection for selectivity Post-column derivatization with reagent containing Vanillin allows detection of Monesin, Narasin and Salinomycin at 520 nm with high sensitivity. To increase reagent life it is recommended to use 2-reagent system: Reagent 1: Concentrated Sulfuric Acid – Methanol (4:96) Reagent 2: 60 g/L of Vanillin in Methanol. The HPLC method is isocratic with Methanol/Acetic acid, on a reversed-phase C18 column at 40° C.

Chromatographic Grade Vanillin meets the exacting purity requirements for a post-column chemical, ensuring that detection at 520 nm will be free of reagent artifacts. Because it is sensitive to oxygen, Pickering Laboratories Vanillin is sealed under CO₂ in convenient 30-gram bottles.

Due to the corrosive nature of the reagent, a Pickering Laboratories PCX5200 post-column derivatization instrument is available, with an inert, non-metallic flow path, a pump with piston wash and a 1.4 mL reactor. This instrument may be connected to a standard liquid chromatograph (HPLC) with a UV/Vis detector, to convert it to an analyzer specific for the analysis of polyether antibiotics.

Vanillin 4-Hydroxy-3-methoxybenzaldehyde CAS No. [121-33-5]

REFERENCE
1. H.M. Rodewald, et al., JAOAC Int’l., 1992, 75, 272-279
2. M.R. LaPointe and H. Cohen, JAOAC, 1988, 71, 480-484

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