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(DMAB; Ehrlich's Reagent)
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INTRODUCTION
For Post-column Analysis of Sulfa
Drugs in Feeds
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Chromatographic Grade® pure, colorless crystals
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450 nm detection for selectivity
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Available in 5 gram bottles
Reversed-phase LC followed by post-column derivatization
is used to determine the residue levels of Sulfamethazine,
Sulfathiazole and related compounds in feeds and feed premixes.
Although these compounds have a UV chromphore, so do most
of the other constituents in a complex sample matrix. Forming
a colored derivative post-column allows shifting of the detection
wavelength into the visible range, thus greatly enhancing
selectivity.
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p-Dimethylaminobenzaldehyde (DMAB; Ehrlich’s Reagent) reacts
rapidly with many primary amines to form a complex with maximum
absorbance at 450 nm. Most commercially available grades are not
of sufficient purity to operate in the demanding environment of
post-column chemistry, as demonstrated by excessive baseline noise
and shortened reagent life.
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Pickering Laboratories’ highly purified, Chromatographic
Grade DMAB, begins with the purest commercial grade available.
The post-column reagent is prepared by dissolving DMAB in
glacial acetic acid, methanol and water2:
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DMAB 1.5 g
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Acetic acid 100 mL
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Methanol 60 mL
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Water 40 mL
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DMAB 4-Dimethylamiobenzaldehyde
CAS No. [100-10-7] |
By maintaining the prepared reagent under inert gas, and in a reservoir
fitted with oxygenimpermeable tubing, it is possible to extend its
effectiveness for up to one week.
The Pickering Laboratories AO3100 derivatization instrument (with
a special 0.5 mL reactor) may be connected to a standard liquid
chromatograph (HPLC) with a UV/Vis detector, to convert it to an
analyzer specific for the analysis of sulfa drugs.
References
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R.W. Stringham, E.C. Mundel and R.L. Smallidge, JAOAC, 1982,
65.(4), 823–827.
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R.C. Smallidge, E.J. Kentzer, R.W. Stringham, et al., JAOAC,
1988 71(4), 418–453.
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L.V. Bui, JAOAC, 1993, 76(5), 966.
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