| Pickering Laboratories has reduced the sample preparation time to a quarter of the traditional method. This gives you the green light to analyze more Glyphosate and AMPA in crops than ever before. Why a new way was needed
The traditional method1 for analysis of Glyphosate and AMPA in crops suffered from an expensive, time-consuming cleanup procedure that had less than ideal recoveries. Although the analysis (after clean up) by ion exchange Chromatography with postcolumn derivatization was rugged and sensitive, a new method was sought to improve the sample preparation. This resulted in AOAC Method 2000.52 which has a streamlined cleanup followed by precolumn derivatization and GC/MS analysis. Pickering Laboratories utilizes this new sample preparation to make it suitable for the classic ion-exchange/post-column analytical protocol. The cleanup method is simplified by eliminating the derivatization with hepta fluorobutanol, thus cutting cleanup time 75%. The recovery is us as robust, providing a good detection limit and an excellent high signal to noise ratio. |
Sample preparation summary
You start the extraction by blending the sample with water and centrifuge. Remove nonpolar co-extractives with methylene chloride. Add an acidic modifier and centrifuge. For the Cationexchange cleanup, add some of the extract and a mobile phase mix to the clean-up cartridge. Elute. Then evaporate to dryness. Dissolve residue in mobile phase mix. When you use the Pickering Laboratories PCX 5200 post-column system and the specified LC and post-column conditions, Glyphosate appears in 9 minutes and AMPA in 19 minutes.For more Glyphosate information, see this abstract. Remember, with the Pickering Laboratories “Guaranteed Chemistry” and methods for Glyphosate in crops there’s no stopping you. |
