All posts by Pickering Laboratories

Aflatoxins in Feeds

1-cow“Advances in mycotoxin detection and analytic techniques have shown the mycotoxin problem to be much larger and more diverse than once imagined. As the climate changes, as feed materials are being sourced from different parts of the world and novel sources of feed materials are being used the risk to exposure from many toxins simultaneously is now greater than ever before. Even low levels of mycotoxins in animal diets can have a significant impact on livestock production, such as impaired gut health”. This is from the article ‘Mycotoxins: Risk is greater than ever before’ in the magazine All about feed, August 19, 2016. http://www.allaboutfeed.net/Mycotoxins/Articles/2016/8/Mycotoxins-Risk-is-greater-than-ever-before-2859680W/.

Pickering Laboratories can provide some options for laboratories that want to analyze for Mycotoxins. Aflatoxins can be analyzed by iodine derivatization using Pinnacle PCX or Vector PCX or with UVE photochemical reactor. A combination of photochemical reactor and chemical derivatization can be utilized for multi-residue Mycotoxins analysis. Details of these methods can be found at  https://www.pickeringlabs.com/library/method-abstracts-2/#mycotoxins.

Trip to ACIL’s 2016 Annual Meeting in St. Louis!

Every year, Rebecca Smith attends the annual meeting of the American Council of Independent Laboratories (ACIL) on behalf of Pickering Labs.  This year Jim Murphy was able to come along, and we flew over to St. Louis for a great week of educational sessions and networking with lab owners and managers from all over the country!

sl1

ACIL is the trade association that represents commercial scientific and testing laboratories (independent means the members are not government or university labs).  Its members are engaged in testing, product certification, consulting, and research and development.  Affiliated membership is available to manufacturer’s laboratories, consultants, and suppliers to the industry.  Pickering Laboratories has only been an affiliate member since 2010, but next year ACIL will be celebrating its 80th anniversary!

So as you can imagine, when a group of top laboratory executives get together, there is very hard work to get done.  We listened to great speakers on leadership topics, human resources topics, and business improvement topics.  We also “worked very hard” during a Cardinals baseball game, during a tour at the Anheuser-Busch brewery, and while touring the fabulous downtown St. Louis area. 

Networking events are a great opportunity to talk with more experienced lab managers and learn from their wisdom, and Rebecca comes back every year with great new ideas to implement with the Pickering staff.  The ACIL technical program is also a great learning experience for developing business acumen and keeping your leadership fresh.  This year ACIL brought in Jason Greer, who made two hours of Diversity Training fly by in an instant – seriously one of the most engaging speakers we’ve ever seen!  We also were impressed with John Bohm and his talk on evolving your business strategies and standing out to your customers.  The ACIL staff does a great job every year of selecting awesome content – Jim and Rebecca would encourage any and all lab owners or managers to check out the meeting.  We are so happy a customer referred us to ACIL in 2010 and haven’t missed an annual meeting since!

Since Rebecca is an avid equestrian and enthusiastic beer drinker, I’m guessing you can imagine the pictures we have ready to share from this year’s trip: 

sl2sl3

Both of the above are classic shots from the Budweiser Brewery!  And below we see pictures from the St. Louis Cardinals’ fabulous stadium.

sl4 sl5

Needless to say, I’m sure our staff believed we had a really rough week of hard work!  (And just to make matters more convincing, next year the ACIL annual meeting will be held in Portland, Oregon which is known as another fantastic beer hotspot!)

Random Tangent – African Safari Edition!

By Michael Pickering

My wife and I had the great pleasure of traveling to Kenya this year for a couple of weeks.  As Judy and her friend (our traveling companion) are both avid photographers, we’ve got some spectacular pictures to share.  Everyone at Pickering Labs has enjoyed the stories and photos, and I am hoping that you’ll find them entertaining as well.

Judy and I visited five conservancies in total while we were in Kenya, traveling around for the better part of three weeks.  As such, we have thousands of animal photos to share, which I am told is a bit too many for the Pickering Labs webpage.  So, Judy has helped me select several choices for public consumption and we have included links to the places we have traveled for more information than I will detail here.

In Nairobi, we first visited the Sheldrick Wildlife Trust.  This conservancy focuses on the protection and preservation of elephants and rhinos.  We were able to visit with orphaned elephants, and even saw that the baby elephants learn to bottle-feed themselves during their fostering!  In the second picture below, you can see me posing with Mbegu, the orphaned elephant we “adopted” during our visit.  She came to the conservancy at a very young age and injured, but we are happy to report she has made a full recovery and is thriving in her new community of orphaned elephants and their faithful and hardworking keepers.

mp1 mp2

From Nairobi we continued north to Sambaru, where we were able to see a lot of African wildlife!  We watched elephants drinking from wells that the local people maintain for both themselves and the wildlife.  We also saw Grevy zebra (an endangered species) and cheetas when we visited the Lewa Wilderness Camp.  In addition to staying in the lodge, we also visited some of the 62,000 acres of conserved lands there.

mp3 mp4 mp5 mp6

Lewa lands are home to over 70 different animal species and 350 different bird species.  Lewa is particularly famous as a leading rhino sanctuary, so I wanted to share a photo of the Black Rhino with you.  We also learned that of the 3,000 Grevy zebra remaining worldwide, a full 20% make their home on this conservancy.  The birds we spotted onsite were spectacular!  Here is a picture of the lilac-breasted roller and a pair of crowned cranes for your enjoyment.

mp7 mp8 mp9 mp10

Inspired by the multitudes of birds surrounding us, I was also eager to take flight!  Lewa has an amazing biplane, and although Judy remained earthbound, I was able to take a ride in the skies and view the wildlife from a whole new vantage point!  The biplane experience came complete with the classy attire necessary to remain comfortable during the ride, much to my wife’s delight.

mp11 mp12

We travelled to a Maasai village and the local warriors performed traditional dancing to welcome me to their village.  You can see in the photo below how incredibly high they jump!  Judy has video of this dancing, and my attempts to join in on the fun, but there are some visuals best left to the imagination.  Let’s just say that when Judy tells this story at the lab, everyone is cracking up by the time she’s done.

mp13 mp14 mp15 mp16

From there, we crossed the equator and visited Ol Pejeta Conversancy and saw lions and warthogs pictured above.  Ol Pejeta is the largest Black Rhino conservancy in East Africa, with 108 Black Rhinos on site.  We also visited their Endangered Species Enclosure and below you will see a picture of me meeting the last male Northern White Rhino on the planet. 

Cmp17soloonsidered extinct in the wild, the last three Northern White Rhinos are protected at Ol Pejeta, where they are kept under 24-hour armed guard and enjoy a 700-arce enclosure.  Unfortunately, breeding efforts have proven unsuccessful – it has been determined that the females, Fatu and Najin, are unable to naturally reproduce.  Sudan, the 43-year old male in the picture, is an older fellow and his sperm count is pretty low…  But there is hope that artificially-assisted reproduction is a possibility and the Northern White Rhino subspecies can be saved from complete extinction.  This is an international effort, and you can learn more about the efforts taking place right here in California on the San Diego Zoo’s webpage

From Ol Pejeta, we flew to the Masai Mara Reserve, which is where the prey animals cross the Mara River.  Although the Wildebeest migration occurs between July-October, we missed the massive herds moving through.  Instead, we saw a whole lot of hippos!  You can see them below, and notice the baby sunbathing with mom!  We also took photos of more cheetahs and lions.  I won’t share those particular pictures, but these lions were definitely an actively mating pair!

mp18 mp19 mp20

After Ol Pejeta, we traveled to our final stop at the Amboseli National Park.  We found the highlight of our time there to be the herds of Maasai giraffe.  Particularly fun was watching them drinking from a pond, as you can see in these pictures.

mp21 mp22

mp23On our very last day in Kenya, we were treated to a rare treat.  I was sitting outside the lodge, soaking in the sights and sun, when I spotted movement at the edge of the lawn.  There was some distance of grass forming a manmade lawn, and from there a clear edge to more natural foliage, fallen leaf debris and assorted bush.  Running parallel to the lawn, I watched as something moved just exactly along the edge.  Of course I needed to evaluate from a closer distance, so I called to Judy to bring the camera and set out across the grass.  A snake!  How excellent!  By the time Judy arrived with the camera it had moved off some ways into the brush, but we were able to take enough pictures that, combined with my detailed descriptions, the lodge safari guide was able to find the snake in his book: a black-necked spitting cobra!  Truly a delightful find. 

 

Chromatography Quiz #25

Chromatography Quiz #24 Results

1-fit-bitPickering Labs would like to congratulate the winners of our last newsletter’s Glyphosate Quiz: David Green from Pepperdine University Natural Science Division, Jim Balk from the DHHS Public Health Environmental Laboratory, Narjes Ghafoori from LA County Environmental Toxicology Lab, Karissa Scroggins from North Coast Laboratories, Helene Lachance from Shur-Gain Nutreco, Tom Schneider from Suffolk County Water Authority, and Irene Taylor from Orange County Utilities Water Division Laboratory! 

They have each won and will shortly be receiving a: Fitbit Flex 2 wristband tracker! An ultra-slim, light-weight wristband for daily activity and sleep tracking! This wearable tech goody is also water and swim-proof! Happy tracking to our Quiz Winners!

Thank you all for your submissions!    

 

  

The correct answer to the Polyether Antibiotics Analysis quiz:

The correct answer to the Glyphosate Analysis Quiz was: we injected a sample at the wrong pH. The samples should be at pH 2 for Glyphosate analysis, especially at high injection volumes. A neutral pH sample can cause peak broadening and sometimes the Glyphosate peak elutes as a doublet. The later eluting AMPA peak is less affected by sample pH problems.

We received really great submissions on this quiz from our contestants that included other reasons for misshapen Glyphosate peaks, so we are included for a couple here for additional troubleshooting background/advice!  Nice work everyone!

From one submission, we learn that metal contamination can also affect peak shape: Glyphosate is a pretty good chelating agent through the phosphate end so contamination with transition metals, especially iron, will affect the peak shape of Glyphosate and AMPA with Glyphosate being the first peak impacted.  In the case of metal contamination Pickering recommends flushing the column with Restore, the solution formulated to remove transition metals and to bring the column back to “peak” performance.

Another scientist has pointed out a different common reason for Glyphosate peak shape problems and retention time shift: Extra-column band broadening. Extra-column band broadening will affect more dramatically the early eluting glyphosate peak. If all the peaks are affected in the chromatogram, it would suggest that a problem is related to either the system or the column. As this is not the case here and only the early eluting peaks are affected, it suggests that the problem could lay with the fluid path – fittings, void in guards or sample/mobile phase solvent, etc.  It is always a good idea to make sure all the tubing connections are made correctly; there are no bubbles in the lines or guard column and the injector stator/rotor seal are not worn or defective.

Chromatography Quiz #25: B@$eL!nE Noise!

What is causing the baseline noise in the chromatogram below?  Simply email your answer as well as your full contact information to Rebecca at rlsmith@pickeringlabs.com by January 1st, 2016 in order to win.  You will receive email confirmation that your submission has been received.  The answer to the quiz and winner congratulations will be published in the next issue (to be anonymous, please notify Rebecca in submission). 

Amino Acid Analysis – Baseline Noise

Pinnacle PCX post-column instrument is being used, in a traditional HPLC setup as recommended by Pickering Laboratories. The quiz question: what is causing the baseline noise? 

Post-column conditions for Lithium Amino Acid analysis:
Reagent 1: Trione
Reactor: 130 °C, 0.5 mL
Reagent flow rate: 0.3 mL/min
Injection volume: 10uL

DAD Settings:
Signal = 570nm
Reference = Off

HPLC Flowrate: 0.35mL/min
Column Temperature: 37 °C

   

1-quiz-25

 

 

 

 

 

2016 Environmental Measurement Symposium

NEMC-PhotoRebecca Smith and David Mazawa hopped on a quick flight down to John Wayne Airport earlier this month, but they weren’t heading to Disneyland!  Pickering Laboratories, Inc. was proud to sponsor and exhibit at the 2016 Environmental Measurement Symposium, which is the combined meeting of the Forum on Environmental Accreditation and the National Environmental Monitoring Conference (NEMC).  This year’s meeting was held at the Hyatt Regency in Orange County, CA, a short five minute drive from the happiest place on earth.  Not that we took the time to ride the Matterhorn… We were hard at work, of course!

When not enjoying conversations with customers and attendees at our booth, we attended many enlightening talks which covered all ranges of topics from medical marijuana to metals speciation…  Additionally, the focus this year on Citizen Science was particularly engaging.  Rebecca learned that 15% of people living in the U.S. are drinking unregulated water – private wells are much more prevalent than she previously thought!  And one speaker in particular was even able to directly tie in Pokemon Go!

Rebecca and David presented a poster on Glyphosate Analysis in Soy Beans, Corn and Sunflower Seeds which had been a big hit at the North American Chemical Residue Workshop in Florida.  They found, however, that the booth raffle for a Kindle Oasis was a much bigger draw than the poster presentation!  Attendees had three days to enter their business cards for the raffle, and during lunch on Wednesday we had our fabulous booth neighbor Bridget Wallace at XOS draw the big winner…

The winner of our Kindle Oasis raffle was Houri Mandjikian from LA Department of Water and Power. Congratulations Houri!  She was able to pick up her new Kindle Oasis at the booth during our sponsored afternoon break on Wednesday.  Thank you to Houri and all of our participants in the raffle.

So, with NEMC 2016 wrapped up, you’re probably wondering what we’re up to next?!

Rebecca will be heading to St. Louis next month to participate in the ACIL Annual Meeting.  She’s looking forward to seeing some of you there… and getting a chance to watch the St. Louis Cardinals on their home field!  (Her grandfather is a huge Cardinals fan from his childhood.)

David is looking ahead to AOAC International 2016 and taking a trip to Dallas.  He and Mike Gottschalk will be there, along with our AOAC-involved chemist Maria Ofitserova who always has a busy schedule during the meeting.  Maria recently had her Theanine method accepted as an official AOAC method, so I am sure she will be excited to answer any questions about it!

We hope to see you at one of our next shows!

 

North American Chemical Residue Workshop (NACRW)

NACRWThe North American Chemical Residue Workshop (NACRW), formerly the Florida Pesticide Residue Workshop, was held in St. Pete’s Beach, Florida.  For the 52nd year, professionals met to discuss the latest trends in analysis of pesticides, veterinary drug and other chemical residues.  Matrices of interest included food, animal feed and environmental samples, and everyone came to learn and share the latest in residue analysis.

This year, the big topic was the pending testing regulations for pesticides in cannabis.  The United States now has 33 states that have legalized either medical marijuana or recreational marijuana, and many of these states are moving towards the adoption of these new regulations.  Along with this increasing availability of cannabis products comes the need for regulation and assurance of safety from pesticides residues.  Some states are modeling the regulations closely to tobacco standards, while other states have chosen alternative paths towards formulating their regulations.

Glyphosate has been in the news lately because glyphosate residue is showing up in finished food products.  In addition, European testing has found glyphosate in drinking water and the urine of many citizens.  Consider that the World Health Organization (WHO) recently decided to classify glyphosate as a probable carcinogen and the increasing interest in glyphosate testing becomes even more understandable.  

At the NACRW conference, there was considerable interest in Pickering Labs’ method for glyphosate analysis because it is robust, established and economical.  Our poster actually generated so much interest that we needed to reprint more application handouts at the hotel copy room!  That’s a nice marketing emergency to have!

Glyphosate Analysis in Soy Beans, Corn and Sunflower Seeds

AOAC International Conference

aoac-2016The AOAC International Conference caters to chemists and analytical professionals from around the world. This year the meeting is in Dallas, Texas from September 16-24, 2016. Pickering Laboratories will be showing our latest applications and products at our AOAC exhibition booth #308. We are also a corporate sponsor and a contributor to several committee meetings throughout the year. Our commitment to AOAC is reflected in the nay products and methods we offer that follow AOAC methods and guidelines.

This year, Maria Ofitserova will be presenting her work on the analysis of Theanine in tea, dietary ingredients and supplements by HPLC with post-column derivatization. This method was developed in response to a call for methods issued by the Stakeholder Panel on Dietary Supplements (SPDS). The post-column Theanine method has undergone Single Laboratory Validation (SLV) and has met the standard method performance requirements set up by the SPDS. In August 2016, Pickering’s Theanine method was evaluated by an Expert Review Panel (ERP) and approved as an AOAC First Action Official Method! The method and the results of single laboratory validation are in print and will be published in the Journal of AOAC International later this fall. If you are attending the show, don’t miss our related talk on Monday morning September 19th at 8:00am!

Following the trend of more testing for glyphosate, we are presenting a poster on Glyphosate Analysis in Foods. The Pickering post-column method was used to expand our application for Glyphosate Analysis in Soy Beans, Corn and Sunflower Seeds to also include oats, wheat flour, eggs and other food matrices. Improved sample clean-up procedures allow for preparing samples for injection in half the time. See our expanded application in our poster presentation on Tuesday, September 20th.

Glyphosate is one of our classic applications, and as discussed in this quarter’s NACRW article, it has become very popular again due to the recent discovery of glyphosate in finished food products and urine of consumers. Find our previous newsletter article on the subject here: FDA Announces New Glyphosate Testing in Food.

Maria, Mike and David hope to see you at the upcoming AOAC International Conference in Dallas. Please stop by and say hello, or wave to Maria if you see her at her presentation Monday or at one of the expert panels she belongs to!