Products

Mycotoxin Sample Clean-up

Overview

img-ma-aflaAFLACLEAN, OTACLEAN, AFLA-OTACLEAN & DONEX CLEAN-UP COLUMNS

IMMUNOAFFINITY & SPE SAMPLE CLEAN-UP COLUMNS

Aflatoxins and Ochratoxin A are produced by fungi, e.g. Aspergillus and Penicillium species. Therefore both toxin types are found together in many foods and animal feeds, e.g. cereals. Deoxynivalenol, also known as Vomitoxin, is a metabolite of various molds of the genus Fusarium (F. colmorum, F. graminearum) and can often be found together with other mycotoxins.

Of significant assistance is the clean-up of extracts by a combined immunoaffinity column (the Afla-OtaCLEAN column) for both Aflatoxins and Ochratoxin A in one step. The subsequent analysis may then be performed by HPLC with postcolumn derivatization or other techniques.

    
   


 

 

 
 

Afla-OtaCLEAN™

Simultaneous Clean-up of Aflatoxins and Ochratoxin A

The combination sample clean-up column Afla-OtaCLEAN™ from LCTech is very tolerant towards many matrices and allows for a comprehensive clean-up of the aflatoxins B1, B2, G1 and G2 as well as Ochratoxin A. The high maximum capacity of 150 ng for Aflatoxin B1 and 200 ng for Ochratoxin A provides a wide measurement range. With a shelf-life of 1 year (from date of manufacture) at room temperature, the storage and use of the columns is very convenient.

  

EXAMPLE RECOVERIES OF AFLATOXIN AND OCHRATOXINS

MATRIX

B1

B2

G1

G2

OTA

Maize (Afla 10 ppb, OTA 14.3 ppb)

107 %

91 %

103 %

75 %

97 %

Rice (Afla 10 ppb, OTA 14.3 ppb)

107 %

93 %

98 %

85 %

101 %

Malt (Afla 10 ppb, OTA 14.3 ppb)

98 %

99 %

97 %

70 %

96 %

Raisins (Afla 10 ppb, OTA 14.3 ppb)

98 %

106 %

101 %

69 %

97 %

     


 

 

 

Aflatoxin

​Sample Clean-up Column for Aflatoxin Analysis

AflaCLEAN™ from LCTech was developed for the sample clean-up of foods, grains, feeds, etc. for aflatoxin analysis using HPLC post-column derivatization or other techniques. The AflaCLEAN™ column is selective for B1, B2, G1 and G2. The maximum loading capacity is 150 ng of Aflatoxin. The shelf-life is 2 years (from date of manufacture) at room temperature. Our cost-effective AflaCLEAN Select columns have a shelf-life of 9 months at 4 ºC.
  

EXAMPLE RECOVERIES OF AFLATOXIN

MATRIX

B1

B2

G1

G2

Peanut Butter

95 %

98 %

93 %

84 %

Peanuts

104 %

94 %

96 %

85 %

Dried Distillers Grain

109 %

97 %

90 %

77 %

Maize

101 %

98 %

103 %

80 %


chart-ma-afla

 

Ochratoxin

SAMPLE CLEAN-UP COLUMN FOR OCHRATOXIN ANALYSIS

OtaCLEAN™ was developed for sample clean-up of foods, grains, feeds, etc. for Ochratoxin A analysis using HPLC or other techniques. The antibody employed possesses a very high Ochratoxin A specificity. This leads to exceptional chromatographic results without any interfering secondary signals and very high recovery rates. Independent of the complexity of the matrix, excellent results can be achieved.

The maximum loading capacity is 200 ng Ochratoxin A. The shelf-life is 2 years (from date of manufacture) at room temperature.

 

 

 
  

EXAMPLE RECOVERIES OF OCHRATOXIN A

MATRIX

RECOVERY RATE

Dried Distillers Grain

102 %

Duran Wheat

92 %

Coffee

100 %

Red Wine

108 %

Rye Bran

91 %

Beer

96 %

Grapes

91 %

Horse Feed

94 %

Wort

107 %

chart-ma-afla2b

DONeX

DONeX Sample Clean-up Columns

Deoxynivalenol, also known as Vomitoxin, is a metabolite of various molds of the genus Fusarium (F. culmorum, F. graminearum) and can often be found on contaminated cereals (wheat, barley, oat).

Generally the toxin is analyzed with HPLC/UV detection or alternatively with HPLC / post column derivatization / fluorescence detection or LC / MS. Whichever method is chosen, a good sample preparation extends life time of the analyzer and of the HPLC column and reduces interferences by matrix components. Moreover the running times of the HPLC system can be reduced for easy matrices from about 25 to 10 minutes by pre-cleaning.

If HPLC/UV detection is used, sensitivity can be dramatically enhanced with larger sample volumes.

The DONeX column has a matrix load of up to 4 grams. It is ideally suited to many different matrices, including corn, barley, wheat, animal feeds, muesli, and bread.

 
DONeX COLUMN RECOVERIES

MATRIX

RECOVERIES [%] DON

Bread

108

Corn

90

Chicken Feed

101

Distillers Grain

91

Oat Flacks

100

Pasta (dried)

105

Poultry Feed

100

Rice

104

Rye

91

Wheat

100

Wheat Bran

99

* All matrices were spiked prior to the extraction. Spiking solution was
incubated for at least 1 h with matrix before extraction was started.
Calculation of recovery is based on subtraction of values obtained
without spiking.

Recoveries for Nivalenol are similar to those observed for Deoxynivalenol.


 

chart-ma-donex

Chromatogram of a cereal-based poultry feed sample,
analyzed with HPLC/post column derivatization/FLD,
sample was spiked with 1 ppm DON, extracted and purified,
evaporated sample (1 g) was resolved in 2 mL HPLC
solvent, 40 μL were injected (0.02 gram matrix equivalents
represent 20 ng DON injected)

 

ELISA Kits

scu-elisa-bOtaREAD™ & AflaREAD™

AFLATOXIN AND OCHRATOXIN ELISA TEST KITS

The contamination of food and feed is a worldwide problem. Under inappropriate storage conditions fungi will produce secondary metabolites like mycotoxins, which often render consumption or further processing impossible. As a result of these toxic and hazardous conditions, analyses must be extremely effective, sensitive and reliable to conform to the strict regulations.

The ELISA Test Kits from LCTech meet and exceed all regulatory requirements. The immunological test procedure is based on antibody-antigen recognition and enzymatic color reaction. It leads to a reliable result after a short incubation period and performs quick and sensitive screening of a variety of food products like rice, corn, cocoa, coffee, peanut butter, beer, wine, liquorice and animal feed.

LCTech ELISA kits are delivered ready-to-use and can directly be used for screening sample extracts without further pre-treatment. These kits consist of a shrink-wrapped, coated ELISA plate, the standard solutions, all necessary reagents and a comprehensive manual to guide you through the effective and easy analysis.

FEATURE HIGHLIGHTS

  • Reduced costs (no analytical instruments needed)
  • Very high sample throughput (96 samples incl. stds. in 130 min)
  • Unmatched Aflatoxin B1 sensitivity (0.2 ppb)
  • Unmatched Ochratoxin A sensitivity (0.6 ppb for liquids)
  • Fast and reliable results
  • Highest reproducibility
  • Long shelf-life (12 months from date of manufacture)
  • Easy handling
  • Wide range of matrices including dried distillers grain
  • Each kit contains 96-well plate, standards and all necessary solutions

OTAREAD OCHRATOXIN A

The LCTech ELISA kit test can be used with solid  liquid matrices. The detection range for solid matrices is 2-20 ppb, for beer 0.6-6 ppb and for wine 0.8-8 ppb.

scu-elisa1           scu-elisa2

Pricing & Ordering

Pricing & Ordering

AFLATOXIN SAMPLE CLEAN-UP COLUMN CATALOG INFORMATIONLogin for Prices

Catalog #

Description

Quantity

10514

AflaCLEAN™, Aflatoxins B1, B2, G1, G2, Pkg 25, 24 month* shelf life at room temp. (Minimum order of 20 packs)

each (24 month* shelf life at room temp. (Minimum order of 20 packs)/bottle)

12058

AflaCLEAN™ SELECT, Aflatoxins B1, B2, G1, G2, Pkg 25, 9 month* shelf life at 4 °C (Minimum order of 20 packs)

each (9 month* shelf life at 4 °C (Minimum order of 20 packs)/bottle)

12062

AflaCLEAN™ SELECT, Aflatoxins B1, B2, G1, G2, Pkg 25, 9 month* shelf life at 4 °C (Minimum order of 20 packs)

each (9 month* shelf life at 4 °C (Minimum order of 20 packs)/bottle)

NOTE: Larger quantities available upon request

AFLACLEAN SELECT COLUMNSLogin for Prices

Catalog #

Description

Quantity

12058

AflaCLEAN™ SELECT, Aflatoxins B1, B2, G1, G2, Pkg 25, 9 month* shelf life at 4 °C (Minimum order of 20 packs)

each (9 month* shelf life at 4 °C (Minimum order of 20 packs)/bottle)

12059

AflaCLEAN™ SELECT, Aflatoxins B1, B2, G1, G2, Pkg 500, 9 month* shelf life at 4 °C (Minimum order of 4 packs)

each (9 month* shelf life at 4 °C (Minimum order of 4 packs)/bottle)

12062

AflaCLEAN™ SELECT, Aflatoxins B1, B2, G1, G2, Pkg 25, 9 month* shelf life at 4 °C (Minimum order of 20 packs)

each (9 month* shelf life at 4 °C (Minimum order of 20 packs)/bottle)

12063

AflaCLEAN™ SELECT, Aflatoxins B1, B2, G1, G2, Pkg 500, 9 month* shelf life at 4 °C (Minimum order of 4 packs)

each (9 month* shelf life at 4 °C (Minimum order of 4 packs)/bottle)

Maximum loading capacity of 300 ng of Aflatoxin. Shelf life is 9 months at 4 ºC.

  

AFLACLEAN SMART CARTRIDGESLogin for Prices

Catalog #

Description

Quantity

12862

AflaCLEAN™ SMART, Aflatoxins B1, B2, G1, G2, Pkg 100, 9 month* shelf life at 4 °C (Minimum order of 8 packs)

each (9 month* shelf life at 4 °C (Minimum order of 8 packs)/bottle)

12863

AflaCLEAN™ SMART,, Aflatoxins B1, B2, G1, G2, Pkg 1000, 9 month* shelf life at 4 °C (Minimum order of 2 packs)

each (9 month* shelf life at 4 °C (Minimum order of 2 packs)/bottle)

This innovative design utilizes a syringe for processing and is optimized for faster elution using less solvent. Aflatoxin sample-cleanup for Hazelnut, Peanut, Pistachio, and Corn. 

Maximum loading of 100 ng of Aflatoxin. Shelf life is 6 months at 4 ºC.

 

OTACLEAN COLUMNSLogin for Prices

Catalog #

Description

Quantity

10515

OtaCLEAN™, Ochratoxin A, Pkg 25, 24 month* shelf life at room temp. (Minimum order of 20 packs)

each (24 month* shelf life at room temp. (Minimum order of 20 packs)/bottle)

11022

Afla-OtaCLEAN™, Aflatoxins B1, B2, G1, G2 & Ochratoxin A, Pkg 25, 18 month* shelf life at room temp. (Minimum order of 20 packs)

each (18 month* shelf life at room temp. (Minimum order of 20 packs)/bottle)

11535

OtaCLEAN™, Ochratoxin A, Pkg 500, 24 month* shelf life at room temp. (Minimum order of 4 packs)

each (24 month* shelf life at room temp. (Minimum order of 4 packs)/bottle)

12425

OtaCLEAN™, Ochratoxin A, Pkg 25, 24 month* shelf life at room temp. (Minimum order of 20 packs)

each (24 month* shelf life at room temp. (Minimum order of 20 packs)/bottle)

12427

OtaCLEAN™, Ochratoxin A, Pkg 500, 24 month* shelf life at room temp. (Minimum order of 4 packs)

each (24 month* shelf life at room temp. (Minimum order of 4 packs)/bottle)

Maximum loading capacity of 200 ng of Ochratoxin A. Shelf life is 2 years at room temperature. 
  

OTACLEAN SMART CARTRIDGESLogin for Prices

Catalog #

Description

Quantity

13346

OtaCLEAN™ SMART, Ochratoxin A, special small format, Pkg 100, 9 month* shelf life at 4 °C (Minimum order of 8 packs)

each (9 month* shelf life at 4 °C (Minimum order of 8 packs)/bottle)

13351

OtaCLEAN™ SMART, Ochratoxin A, special small format, Pkg 1000, 9 month shelf life* at 4 °C (Minimum order of 2 packs)

each (9 month shelf life* at 4 °C (Minimum order of 2 packs)/bottle)

This innovative design utilizes a syringe for processing and is optimized for faster elution using less solvent. Shelf life is 6 months at 4 ºC.
  

DONeX COLUMNSLogin for Prices

Catalog #

Description

Quantity

12792

DONeX™, SPE Clean-up Cartridge for DON Analysis, Pkg 25, no expiration at room temp. (Minimum order of 20 packs)

case (no expiration at room temp. (Minimum order of 20 packs)/bottle)

12793

DONeX™, SPE Clean-up Cartridge for DON Analysis, Pkg 500, no expiration at room temp. (Minimum order of 4 packs)

case (no expiration at room temp. (Minimum order of 4 packs)/bottle)

Maximum load capacity of 4 g of DON. No expiration date.
 

ELISA KITS CATALOG INFORMATIONLogin for Prices

Catalog #

Description

Quantity

11139

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11853

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11068

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11854

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