Overview
Pinnacle PCX
POST-COLUMN DERIVATIZATION INSTRUMENT
Part of the complete integrated system of instruments, chemicals, columns, methods and support from Pickering Laboratories. Pinnacle PCX is custom designed for post-column applications matching chemistry, columns and application to optimize sensitivity and reliability of the results.
MULTI-RESIDUE ANALYSIS OF DEOXYNIVALENOL, AFLATOXINS, OCHRATOXIN A, ZEARALENONE AND FUMONISIN BY HPLC AND POST-COLUMN DERIVATIZATION
Although Aspergillus (Aflatoxins, Ochratoxin A) are generally associated with peanuts and Fusarium (Deoxynivalenol, Zearalenone) with wheat, these fungi and those that produce other toxins are not host-selective and so can cross plant species. When infected grains are processed, any visible mold is lost but the toxic metabolites carry over into the finished products. Thus, multi-residue analytical screens for toxins in grain and finished goods are a wiser choice than single-family protocols.
We present a single screen to cover five families of toxins. This method is suitable for analyzing beverages, grains and feeds. The results of a single laboratory validation study of this method for corn grain were published in the Journal of AOAC International (Vol. 92, No. 1, 2009).
TABLE 1. DATA FOR SPIKED CORN SAMPLE
MYCOTOXIN
SPIKE
CONC,
PPB
NATURAL
CONTAMINATION
LEVEL, PPB
RECOVERIES %
% RSD
N=10
Aflatoxin B1
5.0
–
74
19.4
Aflatoxin B2
1.7
–
88
15.4
Aflatoxin G1
5.1
–
84
12.9
Aflatoxin G2
2.2
–
95
13.2
Ochratoxin A
521
–
82
5.5
Zearalenone
690
205
86
10.8
Fumonisin B1
529
3540
113
8.9
Fumonisin B2
102
1109
84
11.6
Deoxynivalenol
930
–
92
7.7
MULTI-RESIDUE MYCOTOXIN ANALYSIS OF DRIED DISTILLERS GRAINS
Distillers grains (DG) are the still residues after the ethanol has been collected. Approximately 90% of US production is used in domestic animal feed. Any Mycotoxins present in the fresh corn can be concentrated by a factor of three. Contamination can also occur during storage. This raises concern about the potential animal and human health hazards from the use of Mycotoxincontaminated distillers grains.
Corn entering the ethanol processing plant as well as distillers grains should be routinely tested for Mycotoxin contaminations to ensure compliance with guidelines set by FDA. We present a single screen method to cover 4 families of toxins that could be present in dried distillers grains (DDG).
TABLE 2. RECOVERIES OF N-METHYL CARBAMATES IN FOOD MATRICES
MYCOTOXIN
SPIKE
CONC,
PPB
NATURAL
CONTAMINATION
LEVEL, PPB
RECOVERIES %
% RSD
N=10
Aflatoxin B1
100
–
65
7.6
Aflatoxin B2
3.4
–
79
6.3
Aflatoxin G1
10.2
–
75
9.4
Aflatoxin G2
4.4
–
82
9.1
Ochratoxin A
203
–
89
7.1
Zearalenone
1057
231
75
8.8
Fumonisin B1
1042
801
109
5.8
Fumonisin B2
1379
223
104
6.8
Pricing & Ordering
Pricing & Ordering
Vladimir's Zen Cart table will be inserted here…
