Overview
Carbamate Pesticide Analysis Columns
Pickering Laboratories carbamate columns are guaranteed to produce the separation of carbamate residues, specified by EPA 531.1, EPA 531.2 and AOAC 985.23 methods.
Post-column conditions for pesticide analysis:
Reagent 1: Hydrolysis reagent CB130 or CB130.2 Reagent 2: 100 mg of OPA, 2 g Thiofluor™ in 950 mL of CB910 Reactor 1: 100 °C, 0.5 mL Reactor 2: ambient. 0.1 mL Reagents flow rate: 0.3 mL/min
Detection:
Fluorometer: λex 330 nm, λem 465 nm
The recommended gradient conditions are subject to change without notice. This may happen because of lot specific changes in the columns, or improvements in the overall method.
The recommended gradient for the column will always be included in the column package, and it supersedes the information in this catalog.
High Resolution
High Resolution/Capacity Carbamate Column 0846250 (4.6 X 250 mm), C8, 5 µm
| 1 Aldicarb sulfoxide 2 Aldicarb sulfone 3 Oxamyl 4 Methomyl | 5 3-Hydroxycarbofuran 6 Aldicarb 7 Propoxur 8 Carbofuran | 9 Carbaryl 10 1-Naphthol 11 Methiocarb 12 BDMC (internal standard) |
CONDITIONS FOR AQUEOUS SAMPLES
| Time (Min) | WATER % | MeOH % |
|---|---|---|
| 0 | 100 | 0 |
| 1 | 100 | 0 |
| 1.1 | 82 | 18 |
| 36 | 30 | 70 |
| 39 | 30 | 70 |
| 39.1 | 0 | 100 |
| 41 | 0 | 100 |
| 41.1 | 100 | 0 |
| 55 | 100 | 0 |
FLOW RATE: 1 mL/min, COLUMN TEMP.: 42 ºC, INJECTION VOLUME: Up to 400 µL
CONDITIONS FOR METHANOLIC SAMPLES
| Time (Min) | WATER % | MeOH % |
|---|---|---|
| 0 | 85 | 15 |
| 1 | 85 | 15 |
| 44 | 25 | 75 |
| 44.1 | 0 | 100 |
| 49 | 0 | 100 |
| 49.1 | 85 | 15 |
| 57 | 85 | 15 |
FLOW RATE: 1 mL/min, COLUMN TEMP.: 42 ºC, INJECTION VOLUME: 10 µL
Rapid Analysis
Rapid Analysis Carbamate Column 1846150 (4.6 X 150 mm), 5 µm
CONDITIONS FOR AQUEOUS SAMPLES
| Time (Min) | WATER % | MeOH % |
|---|---|---|
| 0 | 100 | 0 |
| 1 | 100 | 0 |
| 1.1 | 88 | 12 |
| 26 | 30 | 70 |
| 26.1 | 0 | 100 |
| 28 | 0 | 100 |
| 28.1 | 100 | 0 |
| 38 | 100 | 0 |
FLOW RATE: 1 mL/min, COLUMN TEMP.: 42 ºC, INJECTION VOLUME: Up to 250 µL
| 1 Aldicarb sulfoxide 2 Aldicarb sulfone 3 Oxamyl 4 Methomyl | 5 3-Hydroxycarbofuran 6 Aldicarb 7 Propoxur 8 Carbofuran | 9 Carbaryl 10 1-Naphthol 11 Methiocarb 12 BDMC (internal standard) |
CONDITIONS FOR METHANOLIC SAMPLES
| Time (Min) | WATER % | MeOH % |
|---|---|---|
| 0 | 85 | 15 |
| 0.5 | 85 | 15 |
| 28.5 | 30 | 70 |
| 28.6 | 0 | 100 |
| 33.5 | 0 | 100 |
| 33.6 | 85 | 15 |
| 41 | 85 | 15 |
FLOW RATE: 1 mL/min, COLUMN TEMP.: 42 ºC, INJECTION VOLUME: 10 µL
Expanded Resolution
Expanded Resolution Carbamate Column 0840250 (4.0 X 250 mm), 5 µm
CONDITIONS FOR WATER/METHANOL GRADIENT
| Time (Min) | WATER % | MeOH % |
|---|---|---|
| 0 | 85 | 15 |
| 2 | 85 | 15 |
| 42 | 30 | 70 |
| 46 | 30 | 70 |
| 46.1 | 0 | 100 |
| 51 | 0 | 100 |
| 51.1 | 85 | 15 |
| 59 | 85 | 15 |
FLOW RATE: 0.8 mL/min, COLUMN TEMP.: 50 ºC, INJECTION VOLUME: 10 µL for methanolic and up to 400 µL for aqueous samples
| 1 Aldicarb sulfoxide 2 Aldicarb sulfone 3 Oxamyl 4 Methomyl | 5 3-Hydroxycarbofuran 6 Aldicarb 7 Propoxur 8 Carbofuran | 9 Carbaryl 10 1-Naphthol 11 Methiocarb 12 BDMC (internal standard) |
CONDITIONS FOR WATER / ACETONITRILE GRADIENT
| Time (Min) | WATER % | ACN % |
|---|---|---|
| 0 | 90 | 10 |
| 2 | 90 | 10 |
| 46 | 49 | 51 |
| 46.1 | 30 | 70 |
| 49 | 30 | 70 |
| 49.1 | 90 | 10 |
| 59 | 90 | 10 |
FLOW RATE: 0.8 mL/min, COLUMN TEMP.: 50 ºC, INJECTION VOLUME: 10 µL for methanolic and up to 400 µL for aqueous samples
23 Carbamates
Separation of 23 Carbamates Using Column 0840250 (4.0 X 250 mm)
| 1 Aldicarb sulfoxide 2 Aldicarb sulfone 3 Oxamyl 4 Methomyl 5 3-Hydroxy 6 Aldicarb 7 Propoxur 8 Carbofuran | 9 Carbaryl 10 Methiocarb 11 BDMC internal standard 12 Butocarboxim sulfoxide 13 Butocarboxim sulfone 14 Ethiofencarb sulfoxide 15 Ethiofencarb sulfone 16 Butocarboxim | 17 Ethiofencarb 18 Thiofanox sulfoxide 19 Thiofanox sulfone 20 Methiocarb sulfoxide 21 Methiocarb sulfone 22 3-Ketocarbofuran 23 Thiofanox N 1-Naphthol |
CONDITIONS FOR WATER/METHANOL GRADIENT
| Time (Min) | WATER % | MeOH % |
|---|---|---|
| 0 | 85 | 15 |
| 2 | 85 | 15 |
| 42 | 30 | 70 |
| 46 | 30 | 70 |
| 46.1 | 0 | 100 |
| 51 | 0 | 100 |
| 51.1 | 85 | 15 |
| 59 | 85 | 15 |
FLOW RATE: 0.8 mL/min, COLUMN TEMP.: 50 ºC, INJECTION VOLUME: 10 µL for methanolic and up to 400 µL for aqueous samples
Pricing & Ordering
Pricing & Ordering
| |||
Catalog # | Description | ||
|---|---|---|---|
0840250 | Carbamate Column, expanded resolution, C8, 4.0 x 250 mm, includes Carbamate Test Mixture 1700-0063 | ||
0846250 | Carbamate Column, high resolution/capacity, C8 , 4.6 x 250 mm, includes Carbamate Test Mixture 1700-0063 | ||
1846150 | Analytical Column, rapid analysis/QC, 4.6 × 150 mm (with Carbamate Test Mixture 1700-0063) | ||
3106-0578 | Reverse Phase Guard Holder with 3 cartridges 4.3mm | ||
3106-0579 | Reverse Phase Guard Cartridge, 4.3mm, pack of 2 | ||
