Products

Sodium Amino Acid Analysis Columns

Overview

img-ccg-introSodium Amino Acid Analysis Columns

Ion-exchange chromatography followed by post-column derivatization has been a method of choice for amino acid analysis for many years. Pickering ion-exchange columns allow you to obtain consistent results with sensitivity, stability, selectivity and speed. Sodium columns and buffers systems are designed for amino acids analysis of hydrolyzed samples.

    

Post-column Conditions For Amino Acids Analysis:

Reagent: Trione®
Reactor: 130 °C, 0.5 mL
Reagent Flow Rate: 0.3 mL/min

Detection:

UV-Vis Detector: λ=570 nm for primary amino acids
                           λ=440 nm for secondary amino acids
or
Reagent: 300 mg of OPA, 2 g Thiofluor™, 3 mL of 30 %
                Brij® 35 solution in 950 mL of OD104
Reactor: 45 °C, 0.15 mL
Reagent Flow Rate: 0.3 mL/min

Detection:

Fluorometer: λex 330 nm, λem 465 nm
 

The recommended gradient conditions are subject to change without notice. This may happen because of lotspecific changes in the columns, or improvements in the overall method.

The recommended gradient for the column will always be included in the column package, and it supersedes the information in this catalog. Use the program recommended on the column data sheet for the initial testing.

The column oven temperature programming gives additional flexibility when optimizing methods. Using a temperature gradient allows the user to improve separation, shorten analysis time and fine-tune the method for detecting compounds of interest.

  
  

30-Minute

30-Minute High-efficiency Sodium Cation-exchange Column (4.6 X 110 MM)

Catalog Number 1154110T
Use with Cation -Exchange Gard™ Column Protection System 1700-3102
Use for Protein and Oxidized Feeds Hydrolysate

Temperature Gradient

30-min1

1 Aspartic Acid
2 Threonine
3 Serine
4 Glutamic Acid
5 Proline
6 Glycine
7 Alanine
8 Cystine
 9 Valine
10 Methionine
11 Isoleucine
12 Leucine
13 Norleucine
14 Tyrosine
15 Phenylalanine
16 Histidine
17 Lysine
18 Tryptophan
19 Ammonia
20 Arginine
21 Cysteic Acid
22 Methionine Sulfone

 

METHOD FOR PROTEIN HYDROLYSATE SAMPLES

Time Na315 % Na425 % Na640 % RG011 %
0 100 0 0 0
4.0 100 0 0

0

15.0 0 100 0 0
16.0 0 0 100 0
31.0 0 0 100 0
31.1 0 0 0 100
33.0 0 0 0 100
33.1 100 0 0 0
40 100 0 0 0

 

 

COLUMN OVEN PROGRAM

Time (Min) Temperature ºC 
0 46
4 46
9 70
32 70
33 46

HPLC FLOW RATE: 0.6 mL/min
INITIAL TEMP.: 46 ºC
INJECTION VOLUME: 10 mL OF 0.25 mmole/mL STD.
For more information see Method Abstract 380.1 (page 89) and Pickering Amino Acids Brochure.

30-min2

1 Aspartic Acid
2 Threonine
3 Serine
4 Glutamic Acid
5 Proline
6 Glycine
7 Alanine
8 Cystine
 9 Valine
10 Methionine
11 Isoleucine
12 Leucine
13 Norleucine
14 Tyrosine
15 Phenylalanine
16 Histidine
17 Lysine
18 Tryptophan
19 Ammonia
20 Arginine
21 Cysteic Acid
22 Methionine Sulfone

  

METHOD FOR PROTEIN HYDROLYSATE SAMPLES

Time Na315% Na425 % Na640 % RG011 %
0 100 0 0 0
4.0 100 0 0 0
15.0 0 100 0 0
16.0 0 0 100 0
31.0 0 0 100 0
31.1 0 0 0 100
33.0 0 0 0 100
33.1 100 0 0 0
40 100 0 0 0

  

COLUMN OVEN PROGRAM

Time (Min) Temperature ºC
0 55
12 55
17 70
32 70
33 55

HPLC FLOW RATE: 0.6 mL/min
INITIAL TEMP.: 55 ºC
INJECTION VOLUME: 10 mL OF 0.25 mmole/mL STD.
For more information see Method Abstract 380.1 (page 89) and Pickering Amino Acids Brochure.

 

 

High-efficiency

High-Efficiency Sodium Cation-exchange Column (4.0 X 150 MM)

Catalog Number 1154150T
Use with Gard™ Column Protection System 1700-3102
Use For Oxidized Hydrolysate, Protein Hydrolysate, Collagen Hydrolysate

Temperature Isocratic

chart-ccg-1154150Ta

 3 Aspartic Acid
 4 Threonine
 5 Serine
 6 Glutamic Acid
 7 Proline
 8 Glycine
  9 Alanine
11 Valine
13 Isoleucine
14 Leucine
16 Phenylalanine
18 Lysine
19 Ammonia
20 Histidine
22 Arginine
23 Cysteic Acid
24 Methionine Sulfone

 

CONDITIONS FOR OXIDIZED SAMPLES 1154150T

Time (Min) Na270% Na740 % RG011%

0

100

0

0

14

100

0

0

32

20

80

0

32.1

0

100

0

56

0

100

0

56.1 0 0 100
58 0 0 100
58.1 100 0 0
70 100 0 0

FLOW RATE: 0.4 mL/min, COLUMN TEMP.: 50 ºC,
INJECTION VOLUME: 10 µL OF 0.25 µmole/mL STD
.

 

chart-ccg-1154150Tb

1 Methionine-D,L,-Sulfoxide
2 trans-4-Hydroxy-L-Proline
3 Aspartic Acid
4 Threonine
5 Serine
6 Glutamic Acid
   7 Proline
  8 Glycine
  9 Alanine
10 Cystine
11 Valine
12 Methionine
13 Isoleucine
14 Leucine
15 Tyrosine
16 Phenylalanine
17 D,L & allo-Hydroxylysine
18 Lysine
19 Ammonia
20 Histidine
21 Tryptophan
22 Arginine

  

CONDITIONS FOR PROTEIN & COLLAGEN HYDROLYSATE SAMPLES

Time (Min) Na315 % Na740 % RG011 %

0

100

0

0

10

100

0

0

30

0

100

0

53

0

100

0

53.1

0

0

100

55 0 0 100
55.1 100 0 0
67 100 0 0

FLOW RATE: 0.4 mL/min, COLUMN TEMP.: 48 ºC,
INJECTION VOLUME: 10 µL OF 0.25 µmole/mL STD.

 

Standard

Standard Sodium Cation-Exchange Column (3.0 X 250 MM)

Catalog Number 1193250
Use with Cation-Exchange Gard™ Column Protection System 1700 – 3102
Use for Protein Hydrolysate

Temperature Isocratic
  

chart-ccg-1193250

 3 Aspartic Acid
 4 Threonine
 5 Serine
 6 Glutamic Acid
 7 Proline
 8 Glycine
 9 Alanine
10 Cystine
11 Valine
12 Methionine
13 Isoleucine
14 Leucine
15 Tyrosine
16 Phenylalanine
17 Hydroxylysines
18 Lysine
19 Ammonia
20 Histidine
21 Tryptophan
22 Arginine

  

CONDITIONS FOR PROTEIN HYDROLYSATE SAMPLES

Time (Min) Na328 % Na740 % RG011 %

100

0

0

10

100

0

0

32

0

100

0

56

0

100

0

56.1

0

0

100

58 0 0 100
58.1 100 0 0
70 100 0 0

FLOW RATE: 0.3 mL/min, COLUMN TEMP.: 48 ºC, INJECTION VOLUME: 10 µL OF 0.25 µmole/mL STD.

 

GARD™

img-ccg-intro2GARD™ Column Protection System

The new GARD™ uses a proprietary material to prevent matrix compounds from passing through (and thereby protecting the analytical column), but allows the analytes of interest to pass unimpeded through the GARD™ and onto the analytical column.

The new GARD™ significantly prolongs column life without band spreading or added pressure. We demonstrated, by means of a performance comparison for Amino Acid Analysis, that the use of a GARD™ will effectively protect the analytical column, will be more cost-effective for the laboratory, is easy to change, is universal to cation-exchange applications, and most importantly has zero band spreading.

Wth the new GARD™ Column protection system from Pickering Laboratories the same cation-exchange GARD™ can be used for nearly all cation-exchange applications.

Pricing & Ordering

Pricing & Ordering

SODIUM AMINO ACID ANALYSIS COLUMNSLogin for Prices

Catalog #

Description

1154150

High-efficiency Sodium Cation-exchange column for protein and collegen hydrosates, 4.0 × 150 mm & 1700-0070 amino acid test mixture

1154150T

Sodium Ion-exchange Column, 4.0 x 150 mm (with test mixture 1700-0070)

1154110T

30-Minute Sodium Cation-exchange Column 4.6 x 110 mm & 1700-0070 amino acid test mixture

1193250

Sodium Ion-exchange Column, 3.0 x 250 mm (with test mixture 1700-0070)

 

GARD™ COLUMN PROTECTION SYSTEMLogin for Prices

Catalog #

Description

1700-3102

Cation-exchange GARD™ assembly: Holder w/ 2 replaceable GARD™

1700-3101

Replacement Cation-exchange GARD™ (2/PK)

1700-3100

GARD™ Holder

 (For use with any cation-exchange column)