Products

Lithium Amino Acid Analysis Columns

Overview

img-ccg-introLithium Amino Acid Analysis Columns

Pickering Laboratories specialize in manufacturing of cation-exchange columns for amino acid analysis. No other techniques, including reversed-phase chromatography, have been shown to match post-column ion-exchange methods in quantitation and reproducibility. Advantages of this method, such as absence of matrix interferences, are especially important in analysis of native samples.

Lithium columns and buffers systems have high selectivity and are perfect for physiological fluids and food analysis.

Post-column Conditions For Amino Acids Analysis:

Reagent: Trione®
Reactor: 130 °C, 0.5 mL
Reagent Flow Rate: 0.3 mL/min

Detection:

UV-Vis Detector: λ=570 nm for primary amino acids
                           λ=440 nm for secondary amino acids
or

Reagent: 300 mg of OPA, 2 g Thiofluor™, 3 mL of 30 %
                Brij® 35 solution in 950 mL of OD104
Reactor: 45 °C, 0.15 mL
Reagent Flow Rate: 0.3 mL/min

Detection:

Fluorometer: λex 330 nm, λem 465 nm
  

The recommended gradient conditions are subject to change without notice. This may happen because of lotspecific changes in the columns, or improvements in the overall method.

The recommended gradient for the column will always be included in the column package, and it supersedes the information in this catalog. Use the program recommended on the column data sheet for the initial testing.

The column oven temperature programming gives additional flexibility when optimizing methods. Using a temperature gradient allows the user to improve separation, shorten analysis time and fine-tune the method for detecting compounds of interest.

  


  

70-Minute

70-minute High-efficiency Lithium Cation-exchange Column

Catalog number 0354675T
Use With Cation-Exchange Gard™ Column Protection System 1700 – 3102
Use For Physiological Samples

Temperature Gradient
 

HPLC PROGRAM

TIME 1700-1125 % Li365 % Li375 % RG003 %

0

100

0

0

0

10

100

0

0

0

19

40

60

0

0

32

0

100

0

0

43

0

100

0

0

43.1

0

0

100

0

57

0

0

100

0

57.1

0

0

70

30

72

0

0

70

30

72.1

100

0

0

0

HPLC FLOW RATE: 0.55 mL/min, INITIAL TEMP.: 34 ºC

COLUMN OVEN PROGRAM

TIME TEMP ºC
0 34
6 34
17 65
25 70
70 70
71 34

REAGENT FLOW RATE: 0.5 mL/min ;
For more information see Method Abstract MA 382 (page 90) and Pickering Amino Acids Brochure.

  

  chart-ccg-70

 1 Phosphoserine
 2 Taurine
 3 Phosphoethanolamine
 4 Urea
 5 Aspartic acid
 6 Hydroxyproline
 7 Threonine
 8 Serine
 9 Asparagine
10 Glutamic acid
11 Glutamine
12 Sarcosine
13 α-Aminoadipic acid
14 Proline
15 Glycine
16 Alanine
17 Citrulline
18 α-Amino-n-butyric acid
19 Valine
20 Cystine
21 Methionine
22 Allo-Isoleucine
23 Cystathionine
24 Isoleucine
25 Leucine
26 Tyrosine
27 Phenylalanine
28 β-Alanine
29 β-Amino-i-butyric acid
30 Homocystine
31 γ-Aminobutyric acid
32 Tryptophan
33 Ethanolamine
34 Ammonia
35 Hydroxylysines
36 Ornitine
37 Lysine
38 1-Methylhistidine
39 Histidine
40 3-Methylhistidine
41 Anserine
42 Carnosine
43 Arginine
44 Glucosaminic Acid*
45 2-Aminoethyl-cysteine (AEC)*

*Internal Standard

NOTE: This method utilizes column temperature gradient. Use Pinnacle PCX column oven
or HPLC column oven with temperature gradient capabilities.

 

High-efficiency

High-efficiency Lithium Cation-exchange Column

Catalog number 0354100T
Use with Cation-Exchange Gard™ Column Protection System 1700 – 3102
Use for Physiological Samples and Complex Matrices

Temperature Isocratic

chart-ccg-lithium

1 Phosphoserine
 2 Taurine
 3 Phosphoethanolamine
 4 Urea
 5 Aspartic acid
 6 Hydroxyproline
 7 Threonine
 8 Serine
 9 Asparagine
10 Glutamic acid
11 Glutamine
12 Sarcosine
13 α-Aminoadipic acid
14 Proline
15 Glycine
16 Alanine
17 Citrulline
18 α-Amino-n-butyric acid
19 Valine
20 Cystine
21 Methionine
22 Cystathionine
23 Isoleucine
24 Leucine
25 Norleucine
26 Tyrosine
27 Phenylalanine
28 β-Alanine
29 β-Amino-i-butyric acid
30 Homocystine
31 γ-Aminobutyric acid
32 Tryptophan
33 Ethanolamine
34 Hydroxylysines
35 Ammonia
36 Creatinine
37 Ornithine
38 Lysine
39 Histidine
40 3-Methyl-histidine
41 1-Methyl-histidine
42 Carnosine
43 Anserine
44 α-Amino-β- guanidinopropionic acid
45 Arginine  

 

CONDITIONS

Step Time Interval Li275 % Li750 % RG003 % Comment

0

0

0

100

0

0

Inject

1

8

8

100

0

0

Isocratic

2

46

38

65

35

0

Linear Gradient

3

86

40

0

100

0

Linear Gradient

4

90

4

0

100

0

Isocratic

5

115

25

0

94

6

Linear Gradient

6

122

2

0

94

6

isocratic

7

122.1

0.1

100

0

0

Step Change

8

140

17.9

100

0

0

Re-equilibration

Flow rate: 0.35 mL/min, column temperature 37°C, injection volume 10 µL of 0.25 µmole/mL Std.
For more information see Pickering Amino Acids Brochure.

Temperature Gradient

CONDITIONS

Step Time 1700-1125 % Li365 % Li375 % RG003 % Comment

0

 

 

 

0

 

 

 

100

 

 

 

0

 

 

 

0

 

 

 

0

 

 

 

Inject

1

 

 

 

15

 

 

 

100

 

 

 

0

 

 

 

0

 

 

 

0

 

 

 

Isocratic

 

 

 

2

 

 

 

27

 

 

 

40

 

 

 

60

 

 

 

0

 

 

 

0

 

 

 

Linear Gradient

3

45

0

100

0

0

Linear Gradient

4

60

0

100

0

0

Isocratic

5

60.1

0

0

100

0

Step Change

6

78

0

0

100

0

Isocratic

7

78.1

0

0

70

30

Step Change

8

95

0

0

70

30

Isocratic

9

95.1

100

0

0

0

Step Change

10

115

100

0

0

0

Equilibration

 HPLC FLOW RATE: 0.4 mL/min, INITIAL TEMP.: 34 ºC INJECTION VOLUME: 10 μL of 0.25 μmole/mL Std.

 

COLUMN OVEN PROGRAM

Time (Min) Temperature ºC
0 34
13 34
30 65
67 65
80 70
97 70
98 34
HPLC FLOW RATE: 0.4 mL/min, INITIAL TEMP.: 34 ºC INJECTION VOLUME: 10 μL of 0.25 μmole/mL Std.


 

chart-ccg-0354100T

 1 Phosphoserine
 2 Taurine
 3 Phosphoethanolamine
 4 Urea
 5 Aspartic acid
 6 Hydroxyproline
 7 Threonine
 8 Serine
 9 Asparagine
10 Glutamic acid
11 Glutamine
12 Sarcosine
13 α-Aminoadipic acid
14 Proline
15 Glycine
16 Alanine
17 Citrulline
18 α-Amino-n-butyric acid
19 Valine
20 Cystine
21 Methionine
22 Cystathionine
23 Isoleucine
24 Leucine
25 Tyrosine
26 Phenylalanine
27 β-Alanine
28 β-Amino-i-butyric acid
29 Homocystine
30 γ-Aminobutyric acid
31 Tryptophan
32 Ethanolamine
33 Ammonia
34 Hydroxylysines
35 Ornithine
36 Lysine
37 1-Methyl-histidine
38 Histidine
39 3-Methyl-histidine
40 Anserine
41 Carnosine
42 Anserine 
43 Glucosaminic Acid*

*Internal Standard
NOTE: This method utilizes column temperature gradient.
Use Pinnacle PCX column oven or HPLC column oven with temperature gradient capabilities.
For more information see Pickering Amino Acids Brochure.

Temperature Isocratic

chart-ccg-0354100Tb

 

CONDITIONS

Step Time
(Min)
Interval Li275 % Li750 % RG003 % Comment

0

0

0

86

14

0

Inject

1

25

11

73

27

0

Linear Gradient

3

25.1

0.1

0

0

100

Step Gradient

4

30

16.9

0

0

100

Isocratic

7

30.1

0.1

86

14

0

Step Gradient

8

42

14.9

86

14

0

Re-equilibration

 HPLC FLOW RATE: 0.35 mL/min, COLUMN TEMP.: 38 ºC
INJECTION VOLUME: 10 μL of 0.25 μmole/mL Std.

Standard

Standard Lithium Cation-Exchange Column

Catalog Number 0393250
Use With Cation-Exchange Gard™ Column Protection System 1700 – 3102
Use for Physiological Samples and Complex Matrices

Temperature Isocratic

chart-ccg-0393250

 1 Phosphoserine
 2 Taurine
 3 Phosphoethanolamine
 4 Urea
 5 Aspartic acid
 6 Hydroxyproline
 7 Threonine
 8 Serine
 9 Asparagine
10 Glutamic acid
11 Glutamine
12 Sarcosine
13 α-Aminoadipic acid
14 Proline
15 Glycine
16 Alanine
17 Citrulline
18 α-Amino-n-butyric acid
19 Valine
20 Cystine
21 Methionine
22 Cystathionine
23 Isoleucine
24 Leucine
25 Norleucine
26 Tyrosine
27 Phenylalanine
28 β-Alanine
29 β-Amino-i-butyric acid
30 Homocystine
31 γ-Aminobutyric acid
32 Tryptophan
33 Ethanolamine
34 Hydroxylysines
35 Ammonia
36 Creatinine
37 Ornithine
38 Lysine
39 Histidine
40 3-Methyl-histidine
41 1-Methyl-histidine
42 Carnosine
43 Anserine
44 α-Amino-β- guanidinopropionic acid
45 Arginine  

   

CONDITIONS

Time Li275 % Li750 % RG003 %

0

100

0

0

17

100

0

0

65

65

35

0

128

0

100

0

145

0

100

0

185

0

94

6

185.1

100

0

0

210

100

0

0

FLOW RATE: 0.3 mL/min, COLUMN TEMP.: 40 ºC,
INJECTION VOLUME: 10 µL OF 0.25 µmole/mL STD.

GARD™

img-ccg-intro2GARD™ Column Protection System

The new GARD™ uses a proprietary material to prevent matrix compounds from passing through (and thereby protecting the analytical column), but allows the analytes of interest to pass unimpeded through the GARD™ and onto the analytical column.

The new GARD™ significantly prolongs column life without band spreading or added pressure. We demonstrated, by means of a performance comparison for Amino Acid Analysis, that the use of a GARD™ will effectively protect the analytical column, will be more cost-effective for the laboratory, is easy to change, is universal to cation-exchange applications, and most importantly has zero band spreading.

Wth the new GARD™ Column protection system from Pickering Laboratories the same cation-exchange GARD™ can be used for nearly all cation-exchange applications.

Pricing & Ordering

Pricing & Ordering

LITHIUM COLUMNS CATALOG INFORMATIONLogin for Prices

Catalog #

Description

0354675T

Lithium Ion-exchange Column, 4.6 x 75 mm (with test mixture 1700-0070)

0354100T

Lithium Ion-exchange Column, 4.0 x 100 mm (with test mixture 1700-0070)

O393250

Lithium Ion-exchange Column, 3.0 x 250 mm (with test mixture 1700-0070)

GARD™ COLUMN PROTECTION SYSTEMLogin for Prices

Catalog #

Description

1700-3102

Cation-exchange GARD™ assembly: Holder w/ 2 replaceable GARD™

1700-3101

Replacement Cation-exchange GARD™ (2/PK)

1700-3100

GARD™ Holder

(For use with any cation-exchange column)