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Polyether Antibiotics

Overview

img-ccg-intro2Polyether Antibiotics Analysis Columns

Polyether antibiotics, such as Monensin, Narasin and Salinomycin, found in raw material, premix, liquid supplements and final feeds, are best quantified using HPLC with post-column derivatization. Because of selectivity of the post-column reaction, almost no sample clean-up is needed.

 

 

 

LC Conditions:

Flow Rate:  0.7 mL/min, column temperature
                     40 °C, injection volume 10 μL
Mobile Phase:  90:10 of Methanol / 5 % Acetic Acid
                            in water, isocratic

Post-Column Conditions:
(non-metallic post-column derivatization system is required):

Reagent 1:  Concentrated Sulfuric Acid / Methanol (4:96 v/v)
Reactor 1:  Ambient, 0.1 mL
Reagent 2:  60 g of Vanillin (or p-dimethylaminobenzaldehyde)
                     in 950 mL of Methanol
Reactor 2:  90 °C, 1.4 mL
Reagents Flow Rate:  0.3 mL/min

NOTE: Using 2-reagent system for this application
greatly extends life of reagents.

One-reagent method and post-column derivatization
system are also available.

Detection:

UV-Vis Detector: Vanillin λ=520 nm, DMAB λ=450 nm
 
  

 

 

chart-ccg-paac

 

 

 

 

 

 

 

Pricing & Ordering

Pricing & Ordering

POLYETHER ANTIBIOTICS COLUMNS CATALOG INFORMATIONLogin for Prices

Catalog #

Description

2381750

Polyether Column, 4.6 × 250 mm

18ECG001

Guard Cartridge Holder with 3 cartridges

18ECG002

Guard Cartridges, 2/pk.

 

 
 
 

 

 

 

 

Retention Times
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