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Polyether Antibiotics

Overview

img-ccg-intro2Polyether Antibiotics Analysis Columns

Polyether antibiotics, such as Monensin, Narasin and Salinomycin, found in raw material, premix, liquid supplements and final feeds, are best quantified using HPLC with post-column derivatization. Because of selectivity of the post-column reaction, almost no sample clean-up is needed.

 

 

 

LC Conditions:

Flow Rate:  0.7 mL/min, column temperature
                     40 °C, injection volume 10 μL
Mobile Phase:  90:10 of Methanol / 5 % Acetic Acid
                            in water, isocratic

Post-Column Conditions:
(non-metallic post-column derivatization system is required):

Reagent 1:  Concentrated Sulfuric Acid / Methanol (4:96 v/v)
Reactor 1:  Ambient, 0.1 mL
Reagent 2:  60 g of Vanillin (or p-dimethylaminobenzaldehyde)
                     in 950 mL of Methanol
Reactor 2:  90 °C, 1.4 mL
Reagents Flow Rate:  0.3 mL/min

NOTE: Using 2-reagent system for this application
greatly extends life of reagents.

One-reagent method and post-column derivatization
system are also available.

Detection:

UV-Vis Detector: Vanillin λ=520 nm, DMAB λ=450 nm
 
  

 

 

chart-ccg-paac

 

 

 

 

 

 

 

Pricing & Ordering

Pricing & Ordering

POLYETHER ANTIBIOTICS COLUMNS CATALOG INFORMATIONLogin for Prices

Catalog #

Description

2381750

Polyether Column, 4.6 × 250 mm

18ECG001

Guard Cartridge Holder with 3 cartridges

18ECG002

Guard Cartridges, 2/pk.

 

 
 
 

 

 

 

 

Retention Times
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Open for Business: COVID-19 Update

With the increasingly lengthy disruptions to daily life caused by COVID-19, Pickering Laboratories, Inc. wants to continue reassuring our customers that we remain open for business this summer.  We continue to maximize support for our customers and provide essential businesses with the reagents and supplies necessary to operate, including hospitals, clinical laboratories, food safety and environmental testing facilities.

Our inventory levels and response times remain normal.  Responsiveness is subject to change and new orders still may need to be prioritized accordingly to supply essential businesses. 

Thank you for your patience and for your business as we continue to work together through this difficult time.  Please contact us with any questions you may have.

Customer Service: orders@pickeringlabs.com
Technical Support: support@pickeringlabs.com
Phone: 650-694-6700