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Thiofluor™ 2-(Dimethylamino)ethanethiol HCl


img-genericThiofluor™ 2-(Dimethylamino)ethanethiol HCl 

N,N-Dimethyl-2-Mercaptoethylamine Hydrochloride – Chromatographic Grade™ For Automated Post-Column Derivatization of Primary Amines

Primary amines form highly fluorescent adducts when reacted with o-Phthalaldehyde (OPA) and a mercaptan under basic conditions.

The products of this reaction, 1-alkyl-2-thioalkyl-subsitituted isoindoles, exhibit optimal excitation at 330 nm and maximal emission at 465 nm.

Pickering’s Thiofluor™ 2-(Dimethylamino)ethanethiol HCl, a solid, nearly odorless nucleophile, is a superior substitute for 2-Mercaptoethanol in the preparation of OPA reagents. It forms a more stable reagent and a longer-lasting fluorophore with OPA than does 2-Mercaptoethanol, yet it has the same fluorescence properties.

Unlike the volatile 2-Mercaptoethanol, Thiofluor™ will not migrate through the gas manifold and regulator of the OPA reagent pressurization system.

Two grams of Thiofluor™ 2-(Dimethylamino)ethanethiol HCl is equivalent to 1 mL of 2-Mercaptoethanol.



Pricing & Ordering


Pricing & Ordering


Catalog #




Thiofluor™, 10 g

each (10 g/bottle)








Retention Times

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Open for Business: COVID-19 Update

With the increasingly lengthy disruptions to daily life caused by COVID-19, Pickering Laboratories, Inc. wants to continue reassuring our customers that we remain open for business this summer.  We continue to maximize support for our customers and provide essential businesses with the reagents and supplies necessary to operate, including hospitals, clinical laboratories, food safety and environmental testing facilities.

Our inventory levels and response times remain normal.  Responsiveness is subject to change and new orders still may need to be prioritized accordingly to supply essential businesses. 

Thank you for your patience and for your business as we continue to work together through this difficult time.  Please contact us with any questions you may have.

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