Pickering Laboratories


Thiofluor™ 2-(Dimethylamino)ethanethiol HCl


img-genericThiofluor™ 2-(Dimethylamino)ethanethiol HCl 

N,N-Dimethyl-2-Mercaptoethylamine Hydrochloride – Chromatographic Grade™ For Automated Post-Column Derivatization of Primary Amines

Primary amines form highly fluorescent adducts when reacted with o-Phthalaldehyde (OPA) and a mercaptan under basic conditions.

The products of this reaction, 1-alkyl-2-thioalkyl-subsitituted isoindoles, exhibit optimal excitation at 330 nm and maximal emission at 465 nm.

Pickering’s Thiofluor™ 2-(Dimethylamino)ethanethiol HCl, a solid, nearly odorless nucleophile, is a superior substitute for 2-Mercaptoethanol in the preparation of OPA reagents. It forms a more stable reagent and a longer-lasting fluorophore with OPA than does 2-Mercaptoethanol, yet it has the same fluorescence properties.

Unlike the volatile 2-Mercaptoethanol, Thiofluor™ will not migrate through the gas manifold and regulator of the OPA reagent pressurization system.

Two grams of Thiofluor™ 2-(Dimethylamino)ethanethiol HCl is equivalent to 1 mL of 2-Mercaptoethanol.



Pricing & Ordering


Pricing & Ordering


Catalog #




Thiofluor™, 10 g

each (10 g/bottle)








Retention Times

Follow Us
si-facebook   si-linkedin   si-twitter



Open for Business: COVID-19 Update

As of right now, Pickering Laboratories, Inc. will remain open during the “Shelter in Place” order for Santa Clara County so as to provide other essential businesses with the support and supplies necessary to operate, including hospitals and clinical laboratories.

Our inventory levels and response times are currently normal. Responsiveness is subject to change and orders may need to be prioritized accordingly to supply essential businesses.

Thank you for your patience and for your business as we all work together through this difficult time. Please contact us with any questions you may have.

Customer Service: orders@pickeringlabs.com
Technical Support: support@pickeringlabs.com
Phone: 650-694-6700