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ALKION™ Columns

Overview

img-ccg-introALKION™ Columns

The stationary phase in the ALKION™ column is a rigid, non-porous polymeric phase that is surface sulfonated. The two modes of separation are ion-exchange and moderate partitioning. The low capacity of the ion-exchange resin makes it an ideal phase for the separation of strongly basic and positively charged compounds. The reversed-phase character allows for discrimination between closely related species.

The unique properties of this column allow for its use in a wide range of applications.

 

 

 

Biogenic Amines

 

ALKION™ Column Analysis of Biogenic Amines

Post-Column Conditions:

Reagent: 300 mg of OPA, 2 g Thiofluor™, 3 mL of 30 %
Brij® 35 in 950 mL of OD104
Reactor: 45 °C, 0.15 mL
Reagent Flow Rate: 0.3 mL/min

Detection:

Fluorometer: λex 330 nm, λem 465 nm
  

ALKION-1

ALKION-2

ALKION-3

  
  

CONDITIONS

Time (Min)

K600 %

K563 %

K130 %

0

100

0

0

6

100

0

0

15

0

100

0

21

0

100

0

21.1

0

0

100

23

0

0

100

23.1 100 0 0
29 100 0 0

FLOW RATE: 0.8 mL/min,
COLUMN TEMP.: 45 ºC,
INJECTION VOLUME: 10 µL

 

 

Aminoglycoside Antibiotics

 

ALKION™ Column Analysis of Aminoglycoside Antibiotics in Feeds

Post-Column Conditions:

Reagent: 300 mg of OPA, 2g Thiofluor™, 3 mL of 30 % Brij® 35 in 950 mL of OD104
Reactor: 45 °C, 0.15 mL
Reagents flow rate: 0.3 mL/min

Detection:

Fluorometer: λex 330 nm, λem 465 nm

  

chart-ccg-alkion3

chart-ccg-alkion4

chart-ccg-alkion5

 

chart-ccg-alkion6

  
  

CONDITIONS FOR APRAMYCIN ANALYSIS

Time (Min)

1700-
1101 %

1700-
1102 %

1700-
1103 %

0

67

33

0

5

67

33

0

15

14.7

7.3

78

20

14.7

7.3

78

20.1

0

22

78
21 0 22 78
21.1 67 33 0
28 67 33 0

FLOW RATE: 0.8 mL/min,
COLUMN TEMP.: 40 ºC,
INJECTION VOLUME: 10 µL

 

CONDITIONS FOR GENTAMICIN ANALYSIS

Time (Min)

1700-
1101 %

1700-
1102 %

1700-
1103 %

0

43

31

26

20

9

13

78

30

9

13

78

30.1

0

22

78

31

0

22

78
31.1 43 31 26
38 43 31 26

FLOW RATE: 0.8 mL/min,
COLUMN TEMP.: 40 ºC,
INJECTION VOLUME: 10 µL

 

CONDITIONS FOR NEOMYCIN ANALYSIS

Time (Min)

1700-
1101 %

1700-
1102 %

1700-
1103 %

0

60

40

0

15

13.2

8.8

78

25

11

11

78

25.1

0

22

78

26

0

22

78
26.1 60 40 0
32 60 40 0

FLOW RATE: 0.8 mL/min,
COLUMN TEMP.: 40 ºC,
INJECTION VOLUME: 10 µL

 

CONDITIONS FOR SEPARATION OF APRAMYCIN GENTAMICIN AND NEOMYCIN

Time (Min)

1700-
1101 %

1700-
1102 %

1700-
1103 %

0

60

40

0

15

13.2

8.8

78

15.1

12

10

78

30

12

10

78

30.1

0

22

78
31 0 22 78
31.1 60 40 0
37 60 40 0

FLOW RATE: 0.8 mL/min,
COLUMN TEMP.: 40 ºC,
INJECTION VOLUME: 10 µL

 

 

Streptomycin

 

ALKION™ Column Analysis of Streptomycin

Post-Column Conditions:

Reagent 1: 0.75 N Sodium Hydroxide
Reactor 1: ambient, 0.1 mL
Reagent 2: 0.6 % Ninhydrin
Reactor 2: 60 °C, 0.5 mL
Reagents flow rate: 0.3 mL/min

Detection:

Fluorometer: λex 395 nm, λem 500 nm

Or

Reagent 1: Oxidizing Reagent: 100 μL of 5 % Sodium Hypochlorite in GA116
Reactor 1: 50 °C, 0.5 mL
Reagent 2: 300 mg of OPA, 2 g Thiofluor™, 3 mL of 30 % Brij® 35 Solution in 950 mL of OD104
Reactor 2: ambient, 0.1 mL

Detection:

Fluorometer: λex 330 nm, λem 465 nm

  

chart-ccg-alkion8

 

  
  

CONDITIONS

Time (Min)

1700-
1101 %

1700-
1102 %

1700-
1103 %

0

60

40

0

10

60

40

0

10.1

48

48

4

15

48

48

4

15.1

60

40

0
21 60 40 0

FLOW RATE: 0.8 mL/min,
COLUMN TEMP.: 40 ºC,
INJECTION VOLUME: up to several µL

 

 

 

 

GARD™

img-ccg-intro2GARD™ Column Protection System

The new GARD™ uses a proprietary material to prevent matrix compounds from passing through (and thereby protecting the analytical column), but allows the analytes of interest to pass unimpeded through the GARD™ and onto the analytical column.

The new GARD™ significantly prolongs column life without band spreading or added pressure. We demonstrated, by means of a performance comparison for Amino Acid Analysis, that the use of a GARD™ will effectively protect the analytical column, will be more cost-effective for the laboratory, is easy to change, is universal to cation-exchange applications, and most importantly has zero band spreading.

Wth the new GARD™ Column protection system from Pickering Laboratories the same cation-exchange GARD™ can be used for nearly all cation-exchange applications.

Pricing & Ordering

Pricing & Ordering

ALKION™ COLUMN CATALOG INFORMATIONLogin for Prices

Catalog #

Description

9410917

ALKION™ Cation-exchange Column, K form, 4.0 × 150 mm

9493020

ALKION™ Guard Column, K+ form, 3.0 × 20 mm

GARD™ COLUMN PROTECTION SYSTEMLogin for Prices

Catalog #

Description

1700-3102

Cation-exchange GARD™ assembly: Holder w/ 2 replaceable GARD™

1700-3101

Replacement Cation-exchange GARD™ (2/PK)

1700-3100

GARD™ Holder

(For use with any cation-exchange column) 

 
 
 

 

 

 

 

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Open for Business: COVID-19 Update

As of right now, Pickering Laboratories, Inc. will remain open during the “Shelter in Place” order for Santa Clara County so as to provide other essential businesses with the support and supplies necessary to operate, including hospitals and clinical laboratories.

Our inventory levels and response times are currently normal. Responsiveness is subject to change and orders may need to be prioritized accordingly to supply essential businesses.

Thank you for your patience and for your business as we all work together through this difficult time. Please contact us with any questions you may have.

Customer Service: orders@pickeringlabs.com
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Phone: 650-694-6700