Overview
ALKION™ Columns
The stationary phase in the ALKION™ column is a rigid, non-porous polymeric phase that is surface sulfonated. The two modes of separation are ion-exchange and moderate partitioning. The low capacity of the ion-exchange resin makes it an ideal phase for the separation of strongly basic and positively charged compounds. The reversed-phase character allows for discrimination between closely related species.
The unique properties of this column allow for its use in a wide range of applications.
Biogenic Amines
ALKION™ Column Analysis of Biogenic Amines
Post-Column Conditions:
Reagent: 300 mg of OPA, 2 g Thiofluor™, 3 mL of 30 %
Brij® 35 in 950 mL of OD104
Reactor: 45 °C, 0.15 mL
Reagent Flow Rate: 0.3 mL/min
Detection:
Fluorometer: λex 330 nm, λem 465 nm
CONDITIONS
|
Time (Min) |
K600 % |
K563 % |
K130 % |
|---|---|---|---|
|
0 |
100 |
0 |
0 |
|
6 |
100 |
0 |
0 |
|
15 |
0 |
100 |
0 |
|
21 |
0 |
100 |
0 |
|
21.1 |
0 |
0 |
100 |
|
23 |
0 |
0 |
100 |
| 23.1 | 100 | 0 | 0 |
| 29 | 100 | 0 | 0 |
FLOW RATE: 0.8 mL/min,
COLUMN TEMP.: 45 ºC,
INJECTION VOLUME: 10 µL
Aminoglycoside Antibiotics
ALKION™ Column Analysis of Aminoglycoside Antibiotics in Feeds
Post-Column Conditions:
Reagent: 300 mg of OPA, 2g Thiofluor™, 3 mL of 30 % Brij® 35 in 950 mL of OD104
Reactor: 45 °C, 0.15 mL
Reagents flow rate: 0.3 mL/min
Detection:
Fluorometer: λex 330 nm, λem 465 nm
CONDITIONS FOR APRAMYCIN ANALYSIS
|
Time (Min) |
1700- |
1700- |
1700- 1103 % |
|---|---|---|---|
|
0 |
67 |
33 |
0 |
|
5 |
67 |
33 |
0 |
|
15 |
14.7 |
7.3 |
78 |
|
20 |
14.7 |
7.3 |
78 |
|
20.1 |
0 |
22 |
78 |
| 21 | 0 | 22 | 78 |
| 21.1 | 67 | 33 | 0 |
| 28 | 67 | 33 | 0 |
FLOW RATE: 0.8 mL/min,
COLUMN TEMP.: 40 ºC,
INJECTION VOLUME: 10 µL
CONDITIONS FOR GENTAMICIN ANALYSIS
|
Time (Min) |
1700- |
1700- |
1700- 1103 % |
|---|---|---|---|
|
0 |
43 |
31 |
26 |
|
20 |
9 |
13 |
78 |
|
30 |
9 |
13 |
78 |
|
30.1 |
0 |
22 |
78 |
|
31 |
0 |
22 |
78 |
| 31.1 | 43 | 31 | 26 |
| 38 | 43 | 31 | 26 |
FLOW RATE: 0.8 mL/min,
COLUMN TEMP.: 40 ºC,
INJECTION VOLUME: 10 µL
CONDITIONS FOR NEOMYCIN ANALYSIS
|
Time (Min) |
1700- |
1700- |
1700- 1103 % |
|---|---|---|---|
|
0 |
60 |
40 |
0 |
|
15 |
13.2 |
8.8 |
78 |
|
25 |
11 |
11 |
78 |
|
25.1 |
0 |
22 |
78 |
|
26 |
0 |
22 |
78 |
| 26.1 | 60 | 40 | 0 |
| 32 | 60 | 40 | 0 |
FLOW RATE: 0.8 mL/min,
COLUMN TEMP.: 40 ºC,
INJECTION VOLUME: 10 µL
CONDITIONS FOR SEPARATION OF APRAMYCIN GENTAMICIN AND NEOMYCIN
|
Time (Min) |
1700- |
1700- |
1700- 1103 % |
|---|---|---|---|
|
0 |
60 |
40 |
0 |
|
15 |
13.2 |
8.8 |
78 |
|
15.1 |
12 |
10 |
78 |
|
30 |
12 |
10 |
78 |
|
30.1 |
0 |
22 |
78 |
| 31 | 0 | 22 | 78 |
| 31.1 | 60 | 40 | 0 |
| 37 | 60 | 40 | 0 |
FLOW RATE: 0.8 mL/min,
COLUMN TEMP.: 40 ºC,
INJECTION VOLUME: 10 µL
Streptomycin
ALKION™ Column Analysis of Streptomycin
Post-Column Conditions:
Reagent 1: 0.75 N Sodium Hydroxide
Reactor 1: ambient, 0.1 mL
Reagent 2: 0.6 % Ninhydrin
Reactor 2: 60 °C, 0.5 mL
Reagents flow rate: 0.3 mL/min
Detection:
Fluorometer: λex 395 nm, λem 500 nm
Or
Reagent 1: Oxidizing Reagent: 100 μL of 5 % Sodium Hypochlorite in GA116
Reactor 1: 50 °C, 0.5 mL
Reagent 2: 300 mg of OPA, 2 g Thiofluor™, 3 mL of 30 % Brij® 35 Solution in 950 mL of OD104
Reactor 2: ambient, 0.1 mL
Detection:
Fluorometer: λex 330 nm, λem 465 nm
CONDITIONS
|
Time (Min) |
1700- |
1700- |
1700- 1103 % |
|---|---|---|---|
|
0 |
60 |
40 |
0 |
|
10 |
60 |
40 |
0 |
|
10.1 |
48 |
48 |
4 |
|
15 |
48 |
48 |
4 |
|
15.1 |
60 |
40 |
0 |
| 21 | 60 | 40 | 0 |
FLOW RATE: 0.8 mL/min,
COLUMN TEMP.: 40 ºC,
INJECTION VOLUME: up to several µL
GARD™
GARD™ Column Protection System
The new GARD™ uses a proprietary material to prevent matrix compounds from passing through (and thereby protecting the analytical column), but allows the analytes of interest to pass unimpeded through the GARD™ and onto the analytical column.
The new GARD™ significantly prolongs column life without band spreading or added pressure. We demonstrated, by means of a performance comparison for Amino Acid Analysis, that the use of a GARD™ will effectively protect the analytical column, will be more cost-effective for the laboratory, is easy to change, is universal to cation-exchange applications, and most importantly has zero band spreading.
Wth the new GARD™ Column protection system from Pickering Laboratories the same cation-exchange GARD™ can be used for nearly all cation-exchange applications.
Pricing & Ordering
Pricing & Ordering
| ||
Catalog # | Description | |
|---|---|---|
9410917 | ALKION™ Cation-exchange Column, K form, 4.0 × 150 mm | |
9493020 | ALKION™ Guard Column, K+ form, 3.0 × 20 mm |
| ||
Catalog # | Description | |
|---|---|---|
1700-3102 | Cation-exchange GARD™ assembly: Holder w/ 2 replaceable GARD™ | |
1700-3101 | Replacement Cation-exchange GARD™ (2/PK) | |
1700-3100 | GARD™ Holder |