Products

ALKION™ Columns

Overview

img-ccg-introALKION™ Columns

The stationary phase in the ALKION™ column is a rigid, non-porous polymeric phase that is surface sulfonated. The two modes of separation are ion-exchange and moderate partitioning. The low capacity of the ion-exchange resin makes it an ideal phase for the separation of strongly basic and positively charged compounds. The reversed-phase character allows for discrimination between closely related species.

The unique properties of this column allow for its use in a wide range of applications.


   

 

 

Biogenic Amines

 

ALKION™ Column Analysis of Biogenic Amines

Post-Column Conditions:

Reagent: 300 mg of OPA, 2 g Thiofluor™, 3 mL of 30 %
Brij® 35 in 950 mL of OD104
Reactor: 45 °C, 0.15 mL
Reagent Flow Rate: 0.3 mL/min

Detection:

Fluorometer: λex 330 nm, λem 465 nm
  

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CONDITIONS

Time (Min)

K600 %

K563 %

K130 %

0

100

0

0

6

100

0

0

15

0

100

0

21

0

100

0

21.1

0

0

100

23

0

0

100

23.1 100 0 0
29 100 0 0

FLOW RATE: 0.8 mL/min,
COLUMN TEMP.: 45 ºC,
INJECTION VOLUME: 10 µL

 



 

Aminoglycoside Antibiotics

 

ALKION™ Column Analysis of Aminoglycoside Antibiotics in Feeds

Post-Column Conditions:

Reagent: 300 mg of OPA, 2g Thiofluor™, 3 mL of 30 % Brij® 35 in 950 mL of OD104
Reactor: 45 °C, 0.15 mL
Reagents flow rate: 0.3 mL/min

Detection:

Fluorometer: λex 330 nm, λem 465 nm


  

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CONDITIONS FOR APRAMYCIN ANALYSIS

Time (Min)

1700-
1101 %

1700-
1102 %

1700-
1103 %

0

67

33

0

5

67

33

0

15

14.7

7.3

78

20

14.7

7.3

78

20.1

0

22

78
21 0 22 78
21.1 67 33 0
28 67 33 0

FLOW RATE: 0.8 mL/min,
COLUMN TEMP.: 40 ºC,
INJECTION VOLUME: 10 µL


 

CONDITIONS FOR GENTAMICIN ANALYSIS

Time (Min)

1700-
1101 %

1700-
1102 %

1700-
1103 %

0

43

31

26

20

9

13

78

30

9

13

78

30.1

0

22

78

31

0

22

78
31.1 43 31 26
38 43 31 26

FLOW RATE: 0.8 mL/min,
COLUMN TEMP.: 40 ºC,
INJECTION VOLUME: 10 µL

 

CONDITIONS FOR NEOMYCIN ANALYSIS

Time (Min)

1700-
1101 %

1700-
1102 %

1700-
1103 %

0

60

40

0

15

13.2

8.8

78

25

11

11

78

25.1

0

22

78

26

0

22

78
26.1 60 40 0
32 60 40 0

FLOW RATE: 0.8 mL/min,
COLUMN TEMP.: 40 ºC,
INJECTION VOLUME: 10 µL

 

CONDITIONS FOR SEPARATION OF APRAMYCIN GENTAMICIN AND NEOMYCIN

Time (Min)

1700-
1101 %

1700-
1102 %

1700-
1103 %

0

60

40

0

15

13.2

8.8

78

15.1

12

10

78

30

12

10

78

30.1

0

22

78
31 0 22 78
31.1 60 40 0
37 60 40 0

FLOW RATE: 0.8 mL/min,
COLUMN TEMP.: 40 ºC,
INJECTION VOLUME: 10 µL

 

 

Streptomycin

 

ALKION™ Column Analysis of Streptomycin

Post-Column Conditions:

Reagent 1: 0.75 N Sodium Hydroxide
Reactor 1: ambient, 0.1 mL
Reagent 2: 0.6 % Ninhydrin
Reactor 2: 60 °C, 0.5 mL
Reagents flow rate: 0.3 mL/min

Detection:

Fluorometer: λex 395 nm, λem 500 nm

Or

Reagent 1: Oxidizing Reagent: 100 μL of 5 % Sodium Hypochlorite in GA116
Reactor 1: 50 °C, 0.5 mL
Reagent 2: 300 mg of OPA, 2 g Thiofluor™, 3 mL of 30 % Brij® 35 Solution in 950 mL of OD104
Reactor 2: ambient, 0.1 mL

Detection:

Fluorometer: λex 330 nm, λem 465 nm


  

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CONDITIONS

Time (Min)

1700-
1101 %

1700-
1102 %

1700-
1103 %

0

60

40

0

10

60

40

0

10.1

48

48

4

15

48

48

4

15.1

60

40

0
21 60 40 0

FLOW RATE: 0.8 mL/min,
COLUMN TEMP.: 40 ºC,
INJECTION VOLUME: up to several µL

 

 

 

 

GARD™

img-ccg-intro2GARD™ Column Protection System

The new GARD™ uses a proprietary material to prevent matrix compounds from passing through (and thereby protecting the analytical column), but allows the analytes of interest to pass unimpeded through the GARD™ and onto the analytical column.

The new GARD™ significantly prolongs column life without band spreading or added pressure. We demonstrated, by means of a performance comparison for Amino Acid Analysis, that the use of a GARD™ will effectively protect the analytical column, will be more cost-effective for the laboratory, is easy to change, is universal to cation-exchange applications, and most importantly has zero band spreading.

Wth the new GARD™ Column protection system from Pickering Laboratories the same cation-exchange GARD™ can be used for nearly all cation-exchange applications.

Pricing & Ordering

Pricing & Ordering

ALKION™ COLUMN CATALOG INFORMATIONLogin for Prices

Catalog #

Description

9410917

ALKION™ Cation-exchange Column, K form, 4.0 × 150 mm

9493020

ALKION™ Guard Column, K+ form, 3.0 × 20 mm

GARD™ COLUMN PROTECTION SYSTEMLogin for Prices

Catalog #

Description

1700-3102

Cation-exchange GARD™ assembly: Holder w/ 2 replaceable GARD™

1700-3101

Replacement Cation-exchange GARD™ (2/PK)

1700-3100

GARD™ Holder

(For use with any cation-exchange column)