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Quiz #47 – Piston Washed Out

Here we have a customer with a Pinnacle PCX and they are observing some discoloration in the piston wash solution. Under normal circumstances, the solution has no color and now the solution is yellowish and the piston wash volume continues to increase.

What could be causing this problem with the customers piston wash solution?

Submit your answers to rsmith@pickeringlabs.com by December 31, 2025!

Announcing the winners of our previous quiz, Chromatography Quiz #46 – Peaking at the wrong time

Pickering Laboratories would like to congratulate the winners for our previous newsletter’s Chromatography Quiz #46: Tom Schneider from SCWA, Josiah Hakala from Minnesota Department of Health, and Narjes Ghafoori from Los Angeles County Public Health Laboratory. 

Our winners will shortly be receiving: a $100 gift card from Amazon.com! 

Congratulations to our quiz winners!  Thank you all for your submissions!

 

 

Chromatography Quiz #46 Solution:

For reference, here is what we observed and also what a good chromatogram looks like:

Troubleshooting Chromatogram:

Reference Chromatogram:

For this problem, we take a look at the chemical reaction taking place:

In the first stage, glyphosate is oxidized by hypochlorite to glycine. In the second stage, glycine reacts with o-phthalaldehyde and Thiofluor™ (a mercaptan) at pH 9–10 to produce a highly fluorescent isoindole. AMPA does not need the initial oxidation to react with OPA.

We observed only AMPA and are suspicious of the reagent 1 preparation or possible a physical problem with Pump 1. If there is a problem with the oxidizing reagent, there will be no Glyphosate signal. AMPA does not require oxidation to react with OPA.

 

 

 

Esslab Attends ArabLab

By: Kevin McKeown

In a previous installment of our newsletter, we introduced our new Product Testing Solutions (PTS) aka Artificial Body Fluids dealer for the UK/EU: EssLab

Well, now EssLab has brought the Pickering Test Solutions product line to a new part of the world by featuring us on their booth at ArabLab!

There was a lot of interest in artificial body fluids and this served as the introduction of our products to a new audience of analysts.  ArabLab serves as a meeting point for laboratory equipment manufacturers, life-sciences firms, diagnostics, environmental monitoring, petrochemical testing, food/beverage analysis, and other segments.  This region of the world is making a significant investment in scientific infrastructure, regulatory standards and innovation in analytical/laboratory technology.  We are thrilled that we have a partner who can take our products to countries, and customers, that previously had no easy way of knowing who we are.

This should be a sneak preview of more joint work between Pickering Labs and EssLab.  You can look out for this dynamic duo to be teaming up at conferences throughout the UK/EU in the years to come… maybe even in Munich, Germany in a few short months!

Studying Paralytic Shellfish Toxins in the Pacific Walrus

By: Dr. Maria Ofitserova

Rebecca Smith, President, pictured with her Walrus friend named “Garfield”!

We didn’t want you to think that we only write about our Artificial Body Fluids, so we wanted to pass along an interesting study done utilizing the post-column derivatization method for Paralytic Shellfish Toxins (PSTs).

Alexandrium catenella is a species of dinoflagellates that produce Paralytic Shellfish Toxins that accumulate in bivalves and some fish. As ocean waters warm in the Alaskan Arctics, Harmful Algae Bloom (HAB) caused by rapid increase in population of Alexandrium catenella happens more and more frequently.  Increase in neurotoxins contamination levels of shellfish poses considerable health risk to humans and animals, even causing mass mortality events in large marine mammals.

A study recently published in Harmful Algae journal was using data on paralytic shellfish toxins found in Alexandrium catenella and in shellfish to validate models of PSTs transfer in a critical Arctic food chain and calculate walruses’ toxins exposure during Alexandrium HAB events.

The study was using post-column derivatization method to determine concentrations of toxins in different matrices. Pickering Laboratories’ Onyx PCX derivatization system is ideal for this application and we also offer reagents and HPLC eluants to help laboratories who would like to save time on preparations of the solutions and take advantage of consistent batch to batch performance of consumables manufactures by our company. Application notes MA105 and MA105.1 (https://www.pickeringlabs.com/wp-content/uploads/2021/05/Paralytic-Shellfish-Toxins-MA105.pdf) describe post-column analysis of Paralytic Shellfish Toxins according to official AOAC 2011.02.

The study publication is available here:

https://www.sciencedirect.com/science/article/pii/S1568988325001623

Celebrating 25 Years with Pickering Labs: Anita Gribaldo

By: Christopher Nguyen

This month, we’re proud to celebrate Anita Gribaldo’s 25th work anniversary at Pickering Laboratories!

As our Packaging Lead, Anita has spent a quarter century ensuring every product that leaves our doors meets the highest standards. Her exceptional attention to detail and commitment to quality have made her an indispensable part of our team.

Anita’s reliability, precision, and pride in her work — and in her Polish heritage — reflect the very best of Pickering Labs. We’re grateful for her dedication and the consistency she’s brought to every project over the years.

Congratulations, Anita, on this incredible milestone — and thank you for 25 years of excellence!

New Bioz Partnership for Pickering Labs!

By: Kevin McKeown

You may have seen that our websites: www.pickeringlabs.com and www.pickeringtestsolutions.com have gotten a VERY cool addition: Bioz badges

 

 

 

 

 

 

 

 

 

 

One of the most common questions that we receive from our customer is, “Can I use your artificial body fluids for ______ (insert your sample type or method of analysis!)?”  Previously, we would draw off anecdotal evidence that we may have collected over the years or, in most cases, hesitate to make any suggestions, as our customers know their samples WAY better than we ever could.  Now, we have an easy, searchable way for anyone to explore what our artificial body fluids have been used for. 

From Bioz’s website, here’s the explanation of their service:

Bioz operates a specialized AI-powered search engine designed for life science researchers in academia and the biopharma industry. They mine, analyze and structure huge volumes of scientific literature to provide

What this means for you, and us, is that there is now a way to explore the many ways that our products have been deployed.  Some of our favorites that we have found so far: Small-scale storable paper biobatteries activated via human bodily fluid, Cerumen/Earwax as a means of diagnostic testing, Lung Fluid for the testing of inhaled microplastics and the list goes on!

We encourage you to go hunting yourself and find out about all the different studies that we have been a part of!

Quiz

Quiz #46 – Peaking at the wrong time

In this Glyphosate chromatogram per EPA 547 (link to method abstract), you will see Only the metabolite AMPA eluting around 15 min. What is contributing to the loss of Glyphosate?

Submit your answers to rsmith@pickeringlabs.com by May 30, 2025.

Pickering Standard: 1700-0080 Glyphosate Test Mixture, 2.5 µg/mL, 10 µL injection

Pickering Column: 1954150 Cation-exchange Column for Glyphosate analysis, 4 x 150 mm

Normal Operating Conditions: (for reference only, condition changes may be reflected in chromatogram)

Column Temperature: 55 °C

Flow rate: 0.4 mL/min

Eluent Gradient:

TIME

K200

RG019

0

100

0

15

100

0

15.1

0

100

17

0

100

17.1

100

0

27

100

0

Post-column conditions:

Reagent 1: Oxidizing reagent – 100 uL of 5% Sodium Hypochlorite in 950 mL of GA116 

Reagent 2: 100 mg of OPA, 2 g Thiofluor™ in 950 mL of GA104

Reactor 1: 36 °C, 0.5 mL

Reactor 2: ambient. 0.1 mL

Reagent flow rates: 0.3 mL/min

Detection: Fluorometer ex 330 nm, em 465 nm

Troubleshooting Chromatogram:

Reference Chromatogram:

Announcing the winners of our previous quiz, Chromatography Quiz #45 – Amino Acids Rising Baseline

Pickering Laboratories would like to congratulate the winners for our previous newsletter’s Chromatography Quiz #45: Josiah Hakala from Minnesota Department of Health, Thomas Schneider from SCWA and Narjes Ghafoori from Los Angeles County Public Health Laboratory. 

Our winners will shortly be receiving: a remote-controlled drone, complete with 4k camera! 

Congratulations to our quiz winners!  Thank you all for your submissions!

Chromatography Quiz #45 Solution:

For this problem, we had to review the Onyx pump pressure trace.

We did find a loose tubing on the cap resulting in air being sucked into the pump. The air is initially pushed out at the beginning of the run. You can see the pressure coming up very slowly. Once the reagent is delivered properly, you have a good baseline.

Below you will find the pressure trace once the tubing was secured.

The method details can be found on our website (check out our amino acids brochure).

JIM’S RETIREMENT PARTY

Jim’s Big-Ass Retirement Party 2025

By Rebecca Smith

Welcome sign for the big day!

When Jim Murphy, Chairman and President Emeritus, decided to hang up his hat at the end of the year, we knew there was going to be a bigtime celebration in our near future.  And come February, we didn’t need to twist any arms to have a healthy turnout of well-wishers to send him off into retirement!

David and Christopher, front and center during the staff videos and slideshow.

Each of Pickering’s employees deeply wanted to spend time sharing a memorable story and cheers with our dear Mr. Murphy, so in addition to our surprise farewell video, Jim was very busy during the party chatting with all of our guests.  When I spoke with him after it wrapped, he was overwhelmed with joy and gratitude for these relationships, cultivated over the decades.

Jim with Gloria, our longest tenured employee, who has known Jim for 33 years and counting!

Jim with Severo, Anita and her husband Paul. All friends and colleagues for over two decades.

If you’ve had the opportunity to meet Jim during his 33 years at Pickering, whether you met him at Pittcon in the earlier years or have been by the lab, you’ve likely come to respect and admire him.  He is compassionate and has a great sense of humor.  Jim asks about your family, remembers details in your life, and he’ll help you put air in your tires (ask me how I know).  Jim honored our company’s rich history, but he’s committed to Pickering’s future, and as such, steadily built his succession plan for over a decade.

During his tenure, Jim evolved Pickering Labs into the thriving company it is today from the groundwork of founder Dr. Michael Pickering’s vision.  Jim started in 1991, about a decade into Michael’s greatest experiment (as he called the lab).  And as the early partnership formed, Michael created more new products and met with customers, while Jim secured the people, resources, and finances to execute and support the business’s growth.  Ten years in, this fearsome duo worked together to bring Pickering Labs into it’s forever home at 1280 Space Park, and within our walls, we further blossomed. 

Throwback to Jim and the team celebrating our first Onyx PCX ready to be shipped to the customer!

Jim and Michael in the earlier years.

What a blast! Judy Pickering was not going to miss Jim’s big party!

Throughout the years of Jim’s tenure, the Pickering team has relied on him.  He has been steadfast and true, respectful and welcoming.  He’s shepherded many an instrument through the expensive design and launch phase, tripled the revenue of the company, not to mention interviewed and hired most of our employees, past and present!  Jim has put up with our (my) quirks, he’s challenged us for our best work, and he has touched all of our hearts. 

Jim with his wife Michelle, and Rebecca with my husband, Paul. I’ll dearly miss my daily chats with my longtime mentor.

Please allow us this chance to celebrate with you and share our love for this man who has so powerfully impacted our lives.  Jim’s ensured Pickering’s future with his long-planned and thoughtful transition, and we in turn will do our part in forging ahead to our future. 

Jim, we will always be your family and you’ll forever have a second home at 1280 Space Park!  We love you and profoundly appreciate everything we have learned from you.  You are cherished.  We miss you already… and we cannot wait to see you onsite next month for the Board meeting. 😉

Guaranteed Chemistry