Category Archives: pickering laboratories

4-peak Amino Acids Test Mixture in 0.01 N HCl, 0.25 µmole/mL, each (5x1mL/bottle), L-Cysteic Acid, L-Aspartic Acid, L-Threonine, L-Serine PN 1700-0075

Please take note that part number 1700-0070 Amino Acid Test Mixture is obsolete, effective 17 August 2022. A direct replacement is available. The replacement has an additional amino acid, Aspartic Acid, to aid in Onyx PCX and Vector PCX Performance Qualifications for the amino acids method. Cysteic Acid is not retained by the stationary phase so its retention time may be used to troubleshoot void volume problems. The resolution between Threonine and Serine is used to troubleshoot column health and improper fitting placement in your system. See expected chromatogram below.

Please contact Pickering Laboratories Support at (650)694-6700, (800)654-3330, or support@pickeringlabs.com if you have any questions.

Obsolete Part #

Replacement Part #

Replacement Description

1700-0070

1700-0075

4-peak Amino Acids Test Mixture in 0.01 N HCl, 0.25 µmole/mL, each (5x1mL/bottle), L-Cysteic Acid, L-Aspartic Acid, L-Threonine, L-Serine

Pickering Laboratories Celebrates Our 40th Anniversary!

By: Rebecca Smith

Pickering's 40-year anniversary logo

It is with great pride that we announce the celebration of our 40-year anniversary in 2022! 

As part of our efforts to honor this milestone achievement, we created an interactive timeline of our history.  It can be found at the following link: https://www.pickeringlabs.com/contact-us/about-us/#40-year-anniversary.

Our late friend and founder, Dr. Michael Pickering, began this journey in 1982.  As the namesake of our company, Michael’s many achievements are reflected throughout our timeline but also in our company’s logo and especially in our “Guaranteed Chemistry” watermark, which we’ve updated for this special anniversary.  We continue to uphold his legacy of high quality and dedicated support for our customers. 

As you visit the timeline, you’ll be able to relive the important milestones that we’ve reached during the last four decades, including: a United States patent for our Ninhydrin reagent Trione, introduction of our PCX post-column instrumentation, publishment in official methods and many more highlights.  If you look closely at our shiny new 40th anniversary logo, you may even see a familiar chromatogram or two!

We would like to extend a special “Thank You!” to all of the customers and dealers who have joined us throughout these historic four decades.  We would not be a successful company still today without the trust, partnership and loyalty which you have shown us.  As a small business, we want each of our customers to know that their orders over the years have supported and directly impacted our scientific contributions to the analytical community.  We look forward to our partnership together for many years to come, as well.

And finally, we wish to acknowledge that we could not have made this company a success without our most valuable assets: our employees!  As you may have noticed in the last couple newsletters, our very dedicated colleagues have been celebrating a lot of 20th and 30th work anniversaries lately!  This fantastic team works so hard every day to support our customers; we are so proud and grateful to have them. 

Please, we would be honored if you’d pop the champagne, raise a glass, and celebrate with us! 

Cheers to our Pickering Labs family!  Cheers to our customers and distributors!  Cheers as well to sustaining Michael’s legacy for another 40 years! 

And a clink of our glasses for the scientific work we all do in the world.

Team Time in ‘21

By Christopher Nguyen and Gloria Garcia

Now that we have good COVID-19 vaccination rates among employees at Pickering Labs and less restrictions from the county, we finally got together as a team after over a year of essential work, social distancing, and boxed lunches.  Our breakroom has been closed for months too, so everyone has been eating in their cars or at their desks.  Finally, last week was our first chance to get together as a whole team in the sunshine and fresh air (to enjoy some… boxed lunch LOL). 

Since his hire back in November, Kevin McKeown our new Sales Manager has been remotely working from his home on the East Coast.  Kevin couldn’t pass up a chance to hit California for a week at his earliest opportunity, so we threw him a little [belated] welcome luncheon while he was here. 

Pickering Lunch - group photo 1

Kevin pictured above, meeting Chris and seeing David for the first time since March 2020 at Pittcon.  We wonder… is it any different having lunch with Jim Murphy now that he’s your boss, Kevin?  😉

This was also the perfect opportunity for Kevin and Chris, both pandemic hires, to meet Judy Pickering as well!  She came onsite for lunch and is shown below chatting with Saji, Rebecca and Maria.  Both Gloria and Judy share a love of sunflowers, so we enjoyed some beautiful centerpieces for lunch!

Pickering Lunch - group photo 2

Earlier in the week, Christopher was also finally able to take his Production Team out for a much-deserved lunch!  (Nice of them to invite Kevin along to enjoy good food and even better company.) 

Pickering Lunch - group photo 3

Pictured here: Kevin, Skip, Anita, Severo, Gabriela, Jay, Sareeta, Craig, Ed and Chris.  Good looking crew!

Bread Rises

SF-SourdoughRising to the Occasion
Michael Pickering

My wife Judy baked bread recently for our annual St. Patrick’s Day luncheon at Pickering Labs.  She’s an excellent baker, and her Irish Soda Bread is a favorite addition to the corned beef and cabbage “traditional” fare.  Soda bread uses baking soda instead of yeast as the rising agent for the dough.  In addition to baking soda, her recipe also calls for flour, salt, and buttermilk.  This is typical for soda bread, as the buttermilk contains the lactic acid required for the ‘rising’ reaction with the sodium bicarbonate. 

Having grown up in Southern California, I have fond childhood memories of another special kind of bread – Salt-Rising Bread.  This denser bread relies on the fermentation of salt-tolerant bacteria in cornmeal.  The cornmeal must be freshly stone ground, and the dry ingredients also include sugar and salt.  The starter is formed as scalded milk is poured over the dry ingredients, and then left to incubate for about twelve hours at 100 or so degrees.  Despite its name, salt is a relatively minor ingredient in the bread. 

The Van de Kamp’s bakery had a rich history which straddled my own childhood and years living in the greater Los Angeles area.  Although the bakery was originally sold by the Van de Kamp family back in the 1950’s when Theodore Van de Kamp died, the Dutch windmill style bakeries and fresh salt-rising bread remained a warm memory for many of us Southern California children.  When accompanying my mom to buy our bread, I was treated on more than one occasion to a free windmill toy and a cookie. 

By the mid-1970’s, the Van de Kamp bakeries had stopped baking the salt-rising bread I grew up on, but by that point I had left Southern California and moved up to the Bay Area, a region where the Van de Kamp bread had been seldom offered and shortly went out of business.  However, in San Francisco, sourdough bread reigned supreme and had since the California Gold Rush.

Sourdough bread also uses natural microbes as a rising agent, but the longer fermentation of the starter allows the Lactic Acid produced by the Lactobacillus to give the bread a uniquely sour taste.  Naturally occurring yeasts such as Saccharomyces exigua and Saccharomyces cerevisiae also participate in the rising.  Sourdough yeasts work slower than today’s packaged yeasts, increasing the time needed for fermentation to multiple days.  San Francisco sourdoughs are usually kept closer to 70 degrees and often need a week to become stabilized.

Flour and water are combined in the starter, and various methods for introducing micro-organisms and stabilizing the dough are used.  Often times, boiled potatoes are used to help increase the activity of the bacteria.  Creating a sourdough starter is a baker’s science, and each recipe is unique and starter closely monitored.  As a result, bakers are often using “mother dough” that is many years old.  Some bakeries, such as the Boudin Bakery, are able to trace their “mother dough” back to the Gold Rush era. 

During the 1980’s, as modern food processes and general business consolidation trended, San Francisco bakeries fell into the hardship of competition with prepackaged bread.  Smaller bakeries were driven out of business, and the long-term survivors tended to be the larger bakeries with well-established distribution channels.  I moved to Oregon during this time, and my observations upon returning to the Bay Area some years later made it clear that there was a stark change in the availability of good local sourdough bread.  Fortunately for my family, Judy was at the peak of her baking heyday during this time and we were seldom lacking in good bread around my house!

Fast forward to now, and the artisan bread movement has brought back the ability to purchase good, hand-made loaves of bread.  Specialty bakeries have been started and thrive in high numbers.  Even restaurants and grocery stores are taking the time to bake their own bread.  I personally continue to feel that no sourdough of modern San Francisco origin can compete to the distinct sour taste and texture of earlier days, but there is no Pickering starter dough dating back to 1970 lurking in my refrigerator, so I make do with what’s available.  It is my sincere hope that the continued evolution of artisan bread, gastronomy, and the souring culture will ultimately recreate my ideal sourdough again soon. 

Until then, at least we can look forward to St. Patrick’s Day each year, when Judy will rise to the occasion and bake a unique bread to treat us all again!

  

Replacing the Over-pressure Relief Valve Cartridge

Mixing-Manifold-1a 
Cleaning and Reassembly of the Over-pressure Relief Valve

  1. Remove the tubing connections to the Mixing Manifold. Use a 3/32” hex driver to remove the 2 screws holding the Mixing Manifold to the chassis. Use a 3/8” wrench to remove the end cap and discard the old Over-pressure Relief Valve Cartridge. Ultrasonicate the Mixing Manifold for at least 30 minutes. Rinse well with DI water.
      
      
     
  2. Connect the outlet of your HPLC pump to the Mixing Manifold inlet and pump 100% water at 0.5mL/min to verify the Mixing Manifold is not clogged. If the Mixing Manifold is still clogged after cleaning in an ultrasonicating bath, replace the Mixing Manifold Assembly (PN 1452-0040).
     
  3. Turn off the HPLC flow and make sure there is no pressure on the Mixing Manifold. Insert the new OPRV cartridge, green side down, and screw on the end cap to 20”lbs of torque. To approximate this level of torque, first finger tighten, then tighten an additional 1/8-1/4 turn with a 3/8” wrench.
     
  4. To verify the opening pressure of the Over-pressure Relief Valve, plug the two side inlets of the Mixing Manifold and turn on the HPLC pump to 0.5mL/min. Allow the pressure to slowly rise. The Over-pressure Relief Valve should open around 485psi. If the opening pressure is too low, tighten an additional 1/8 of a turn with a 3/8” wrench.

David Mazawa
david.mazawa@pickeringlabs.com
Technical Support Chemist
Pickering Laboratories, Inc.
1280 Space Park Way
Mountain View, CA 94043 USA
Phone: (650)694-6700 ext. 710
Fax: (650)968-0749

 

Visit Pickering at Pittcon 2014

We will be displaying our equipment at this year’s Pittcon in Chicago, Illinois from March 3-6, 2014. Be sure to stop by:  Booth 2955

We will have our Pinnacle PCX post-column derivatization instrument on display as well as our Mycotoxin Analysis Products. In addition, we will some new videos to highlight the latest in our Automated Sample Preparation instrument the FREESTYLE SPE system.

Wendy, David and Mike will be in Chicago. Please stop by. We would love to talk with you!
fountain

Amino Acid Analysis of Monoclonal Antibodies

Method Abstract 373, Amino Acid Analysis of Monoclonal Antibodies

The peptide and protein based pharmaceuticals are a rapidly expanding class of therapeutical agents that are used to treat a wide variety of health conditions, including cancer, metabolic and auto-immune diseases, HIV and more. Biologic drugs, such as monoclonal antibodies, are derived from living organisms and are usually very expensive. As many biologics are coming off of patents, the market is ready for cost-saving biogenerics. But all proteins, including monoclonal antibodies, have complex structures that determine their function. Differences in structure would alter biological activity leading to changes in safety and efficacy of the drug.

ICH Q6B is a guidance document that provides a set of internationally accepted specifications for biotechnological and biological products to support new marketing applications. It establishes the set of criteria to which a drug substance, drug product or material should conform to be considered acceptable for intended use.

aaa chromatograms from MA373Determining Amino Acid composition following hydrolysis is listed in ICH Q6B as a way to characterize the protein and to confirm its identity by comparing with Amino Acid composition deduced from the gene sequence of the desired product. Amino Acid Analysis data is also used to accurately determine the protein content.

The Amino Acids Analysis with post-column derivatization is a very sensitive, reproducible and rugged method and it has been a preferred approach for laboratories running biological samples, protein, peptides and foods analysis. Pickering Laboratories Inc. offers many Amino Acids Analysis products including post-column derivatization instruments, columns, eluants, reagents and standards. All products are designed to work together to deliver optimum results for any chosen sample.

METHOD
Analytical conditions
Column: High-efficiency Sodium cation-exchange column, 4.6 x 110 mm, P/N 1154110T
Flow Rate: 0.6 mL/min
Mobile Phase: See method in Table 1

Post-Column Conditionsgradient table for AAA monoclonal AB
Post-column System: Pinnacle PCX
Reactor Volume: 0.5 mL
Reactor Temperature: 130 °C
Flow Rate: 0.3 mL/min
Detection: UV/VIS 570 nm for primary amino acids, 440 nm for secondary amino acids
Injection Volume: 10-50 uL

You can download this abstract, as well as our product catalog and other notes from our website: www.pickeringlabs.com