Due to an unfortunate set of circumstances, all submitted answers to the previous Chromatography Quiz on Amino Acid Analysis have been lost. We sincerely apologize for the inconvenience to everyone who submitted an answer, and ask for all entries to be resubmitted. As we genuinely regret our technical difficulties, Rebecca will be sending prizes to all submissions, and grand prizes to everyone with the correct answer. Please resend your email, or resubmit your answer to rlsmith@pickeringlabs.com. Rebecca will send you confirmation via email that she has received your submission. All entries will be reviewed on June 11^th, and prizes will be sent at that time. You can review the quiz at: http://pickeringlabs.blogspot.com/2010/01/chromatography-quiz-no-2.html. Again, our apologies, and if you have any questions please contact Wendy or Rebecca at 650-694-6700.

Chromatography Quiz No. 1:

Special Note: This is the first Quiz included with our newsletter. For each issue, we’ll choose a chromatogram from a different application or industry. So if Carbamates don’t apply to your lab this round, stay tuned!

To Win:
Simply email your answer and your full contact information by December 1st to Rebecca Smith at email Rebecca Smith

The troubleshooting answer and winner congratulations will be published in the next issue.

Chromatography Quiz
Identify the error made when running the Carbamate chromatogram below and win a prize!

Pickering Standard: 1700-0063 Carbamate Test Mix, 2.5 µg/mL, inject 10 µL
Pickering Column: 1846150 Carbamate Column, C18, 4.6 x 150 mm

Normal Operating Conditions (for reference only, condition changes may be reflected in chromatogram):

Column Temperature: 42 °C
Flow rate: 1 mL/min
Eluent Gradient:

Post-column conditions for pesticide analysis:
Reagent 1: Hydrolysis reagent CB130
Reagent 2: 100 mg of OPA, 2 g Thiofluor™ in 950 mL of CB910Reactor 1: 100 °C, 0.5 mL
Reactor 2: ambient. 0.1 mL
Reagent flow rates: 0.3 mL/min
Detection: Fluorometer ex 330 nm, em 465 nm