Chromatography Quiz No. 1: Carbamate Analysis for US EPA 531.1

Chromatography Quiz No. 1:

Special Note: This is the first Quiz included with our newsletter. For each issue, we’ll choose a chromatogram from a different application or industry. So if Carbamates don’t apply to your lab this round, stay tuned!

To Win:
Simply email your answer and your full contact information by December 1st to Rebecca Smith at email Rebecca Smith

The troubleshooting answer and winner congratulations will be published in the next issue.

Chromatography Quiz
Identify the error made when running the Carbamate chromatogram below and win a prize!

Pickering Standard: 1700-0063 Carbamate Test Mix, 2.5 µg/mL, inject 10 µL
Pickering Column: 1846150 Carbamate Column, C18, 4.6 x 150 mm

Normal Operating Conditions (for reference only, condition changes may be reflected in chromatogram):

Column Temperature: 42 °C
Flow rate: 1 mL/min
Eluent Gradient:

Normal Operating Conditions


Post-column conditions for pesticide analysis:
Reagent 1: Hydrolysis reagent CB130
Reagent 2: 100 mg of OPA, 2 g Thiofluor™ in 950 mL of CB910Reactor 1: 100 °C, 0.5 mL
Reactor 2: ambient. 0.1 mL
Reagent flow rates: 0.3 mL/min
Detection: Fluorometer ex 330 nm, em 465 nm

Post-column conditions for pesticide analysis