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Method Abstract 325

MA 325 / ANALYSIS OF FORMALDEHYDE

Formaldehyde is widely used in manufacturing of building  materials, cars, plywood, polymers, glues and adhesives. It is  also commonly used in household items as a preservative and disinfectant. Due to high toxicity and suspected carcinogenicity products are tested to determine free formaldehyde content and ensure the safety of personal care products and other items. Free formaldehyde testing is also important to monitor production of resins and other polymers and to ensure the quality of  final materials.

HPLC method with post-column derivatization allows for quantifying free formaldehyde in a wide range of products and materials, from shampoos to emulsion polymers and phenolic resins. The method is simple, selective and very sensitive. Potential interferences are either separated from formaldehyde on HPLC column or don’t react with post-column reagent and so are not interfering with the detection.

 

METHOD

Analytical Conditions

Column:   Waters Atlantis® HILIC Silica, 5 um, 4.6 x 250 mm 
Temperature: 40  ̊C
Flow Rate: 0.5 mL/min
Mobile Phase:  15% 0.05 M Ammonium Acetate in water  85% Acetonitrile 

Post-Column Conditions

Post-Column System: Pinnacle PCX or Vector PCX 
Reactor Volume: 0.5 mL
Temperature: 100  ̊C
Reagent: 0.81 M Ammonimn acetate 
                0.12 M Galcial acetic acid 
                0.05 M 2, 4-Pentanedione 

Regent Flow Rate: 0.4 mL/min
Detection: FLD λex=412 nm, λem = 510 nm Or UV/Vis  410 nm

ma-325