Glyphosate Sample Clean-up Column

Pickering SPE sample clean-up cartridges are used to treat samples prior to injection. The strong cation-exchange resin is composed of sulfonic acid functional groups attached to a styrene divinylbenzene copolymer lattice. The high selectivity and ruggedness provides a simple and universal method for removing matrix interference from the sample. The SPE sample clean-up cartridges are ideal for the cleanup of vegetables, fruits and crop samples in the analysis of Glyphosate and AMPA.

Recoveries for Glyphosate

MATRIX	GLYPHOSATE	AMPA
Alfalfa	129 %	116 %
Strawberry	84 %	82 %
Broccoli	97 %	95 %

 

 

SAMPLE PREPARATION

Extraction:

Take 25 g of homogenous sample and add enough water (after estimation of moisture content)
to make the total volume of water 125 mL. Blend and Centrifuge.

    

Matrix Specific Modification:

High water content – reduce sample amount to 12.5 g

High protein content – add 100 μL HCl to 20 mL of extract, shake and centrifuge.

High Fat content – perform the methylene chloride partition twice.

CLEAN-UP

Methylene Chloride Partition:

To 20 mL of aqueous extract add 15 mL methylene chloride. Shake for 2-3 min and centrifuge.

To 4.5 mL of aqueous layer add 0.5 mL acidic modifier solution. Shake and centrifuge.

   

SPE Clean-up:

Prepare SPE cartridge (refer to product abstract PA210)

Transfer 1 mL portions of SPE mobile phase (refer to method abstract MA206 for formulation)
and discard the effluent.

 Elute analytes with 12 mL SPE mobile phase

   

Concentration:

Evaporate to dryness using rotary evaporator or a vacuum vortex-type evaporator or lyophilize

Re-dissolve in 2 mL of the SPE mobile phase

Pricing & Ordering

 

 

 

 

 

 

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