Overview
Sodium Amino Acid Analysis Columns
Ion-exchange chromatography followed by post-column derivatization has been a method of choice for amino acid analysis for many years. Pickering ion-exchange columns allow you to obtain consistent results with sensitivity, stability, selectivity and speed. Sodium columns and buffers systems are designed for amino acids analysis of hydrolyzed samples.
Post-column Conditions For Amino Acids Analysis:
Reagent: Trione®
Reactor: 130 °C, 0.5 mL
Reagent Flow Rate: 0.3 mL/min
Detection:
UV-Vis Detector: λ=570 nm for primary amino acids
λ=440 nm for secondary amino acids
or
Reagent: 300 mg of OPA, 2 g Thiofluor™, 3 mL of 30 %
Brij® 35 solution in 950 mL of OD104
Reactor: 45 °C, 0.15 mL
Reagent Flow Rate: 0.3 mL/min
Detection:
Fluorometer: λex 330 nm, λem 465 nm
The recommended gradient conditions are subject to change without notice. This may happen because of lotspecific changes in the columns, or improvements in the overall method.
The recommended gradient for the column will always be included in the column package, and it supersedes the information in this catalog. Use the program recommended on the column data sheet for the initial testing.
The column oven temperature programming gives additional flexibility when optimizing methods. Using a temperature gradient allows the user to improve separation, shorten analysis time and fine-tune the method for detecting compounds of interest.
30-Minute
30-Minute High-efficiency Sodium Cation-exchange Column (4.6 X 110 MM)
Catalog Number 1154110T
Use with Cation -Exchange Gard™ Column Protection System 1700-3102
Use for Protein and Oxidized Feeds Hydrolysate
Temperature Gradient
| 1 Aspartic Acid 2 Threonine 3 Serine 4 Glutamic Acid 5 Proline 6 Glycine 7 Alanine 8 Cystine |
9 Valine 10 Methionine 11 Isoleucine 12 Leucine 13 Norleucine 14 Tyrosine 15 Phenylalanine 16 Histidine |
17 Lysine 18 Tryptophan 19 Ammonia 20 Arginine 21 Cysteic Acid 22 Methionine Sulfone |
METHOD FOR PROTEIN HYDROLYSATE SAMPLES
| Time | Na315 % | Na425 % | Na640 % | RG011 % |
|---|---|---|---|---|
| 0 | 100 | 0 | 0 | 0 |
| 4.0 | 100 | 0 | 0 |
0 |
| 15.0 | 0 | 100 | 0 | 0 |
| 16.0 | 0 | 0 | 100 | 0 |
| 31.0 | 0 | 0 | 100 | 0 |
| 31.1 | 0 | 0 | 0 | 100 |
| 33.0 | 0 | 0 | 0 | 100 |
| 33.1 | 100 | 0 | 0 | 0 |
| 40 | 100 | 0 | 0 | 0 |
COLUMN OVEN PROGRAM
| Time (Min) | Temperature ºC |
|---|---|
| 0 | 46 |
| 4 | 46 |
| 9 | 70 |
| 32 | 70 |
| 33 | 46 |
HPLC FLOW RATE: 0.6 mL/min
INITIAL TEMP.: 46 ºC
INJECTION VOLUME: 10 mL OF 0.25 mmole/mL STD.
For more information see Method Abstract 380.1 (page 89) and Pickering Amino Acids Brochure.
| 1 Aspartic Acid 2 Threonine 3 Serine 4 Glutamic Acid 5 Proline 6 Glycine 7 Alanine 8 Cystine |
9 Valine 10 Methionine 11 Isoleucine 12 Leucine 13 Norleucine 14 Tyrosine 15 Phenylalanine 16 Histidine |
17 Lysine 18 Tryptophan 19 Ammonia 20 Arginine 21 Cysteic Acid 22 Methionine Sulfone |
METHOD FOR PROTEIN HYDROLYSATE SAMPLES
| Time | Na315% | Na425 % | Na640 % | RG011 % |
|---|---|---|---|---|
| 0 | 100 | 0 | 0 | 0 |
| 4.0 | 100 | 0 | 0 | 0 |
| 15.0 | 0 | 100 | 0 | 0 |
| 16.0 | 0 | 0 | 100 | 0 |
| 31.0 | 0 | 0 | 100 | 0 |
| 31.1 | 0 | 0 | 0 | 100 |
| 33.0 | 0 | 0 | 0 | 100 |
| 33.1 | 100 | 0 | 0 | 0 |
| 40 | 100 | 0 | 0 | 0 |
COLUMN OVEN PROGRAM
| Time (Min) | Temperature ºC |
|---|---|
| 0 | 55 |
| 12 | 55 |
| 17 | 70 |
| 32 | 70 |
| 33 | 55 |
HPLC FLOW RATE: 0.6 mL/min
INITIAL TEMP.: 55 ºC
INJECTION VOLUME: 10 mL OF 0.25 mmole/mL STD.
For more information see Method Abstract 380.1 (page 89) and Pickering Amino Acids Brochure.
High-efficiency
High-Efficiency Sodium Cation-exchange Column (4.0 X 150 MM)
Catalog Number 1154150T
Use with Gard™ Column Protection System 1700-3102
Use For Oxidized Hydrolysate, Protein Hydrolysate, Collagen Hydrolysate
Temperature Isocratic
| 3 Aspartic Acid 4 Threonine 5 Serine 6 Glutamic Acid 7 Proline 8 Glycine |
9 Alanine 11 Valine 13 Isoleucine 14 Leucine 16 Phenylalanine 18 Lysine |
19 Ammonia 20 Histidine 22 Arginine 23 Cysteic Acid 24 Methionine Sulfone |
CONDITIONS FOR OXIDIZED SAMPLES 1154150T
| Time (Min) | Na270% | Na740 % | RG011% |
|---|---|---|---|
|
0 |
100 |
0 |
0 |
|
14 |
100 |
0 |
0 |
|
32 |
20 |
80 |
0 |
|
32.1 |
0 |
100 |
0 |
|
56 |
0 |
100 |
0 |
| 56.1 | 0 | 0 | 100 |
| 58 | 0 | 0 | 100 |
| 58.1 | 100 | 0 | 0 |
| 70 | 100 | 0 | 0 |
FLOW RATE: 0.4 mL/min, COLUMN TEMP.: 50 ºC,
INJECTION VOLUME: 10 µL OF 0.25 µmole/mL STD.
| 1 Methionine-D,L,-Sulfoxide 2 trans-4-Hydroxy-L-Proline 3 Aspartic Acid 4 Threonine 5 Serine 6 Glutamic Acid |
7 Proline 8 Glycine 9 Alanine 10 Cystine 11 Valine 12 Methionine |
13 Isoleucine 14 Leucine 15 Tyrosine 16 Phenylalanine 17 D,L & allo-Hydroxylysine 18 Lysine |
19 Ammonia 20 Histidine 21 Tryptophan 22 Arginine |
CONDITIONS FOR PROTEIN & COLLAGEN HYDROLYSATE SAMPLES
| Time (Min) | Na315 % | Na740 % | RG011 % |
|---|---|---|---|
|
0 |
100 |
0 |
0 |
|
10 |
100 |
0 |
0 |
|
30 |
0 |
100 |
0 |
|
53 |
0 |
100 |
0 |
|
53.1 |
0 |
0 |
100 |
| 55 | 0 | 0 | 100 |
| 55.1 | 100 | 0 | 0 |
| 67 | 100 | 0 | 0 |
FLOW RATE: 0.4 mL/min, COLUMN TEMP.: 48 ºC,
INJECTION VOLUME: 10 µL OF 0.25 µmole/mL STD.
Standard
Standard Sodium Cation-Exchange Column (3.0 X 250 MM)
Catalog Number 1193250
Use with Cation-Exchange Gard™ Column Protection System 1700 – 3102
Use for Protein Hydrolysate
Temperature Isocratic
| 3 Aspartic Acid 4 Threonine 5 Serine 6 Glutamic Acid 7 Proline 8 Glycine 9 Alanine |
10 Cystine 11 Valine 12 Methionine 13 Isoleucine 14 Leucine 15 Tyrosine 16 Phenylalanine |
17 Hydroxylysines 18 Lysine 19 Ammonia 20 Histidine 21 Tryptophan 22 Arginine |
CONDITIONS FOR PROTEIN HYDROLYSATE SAMPLES
| Time (Min) | Na328 % | Na740 % | RG011 % |
|---|---|---|---|
|
0 |
100 |
0 |
0 |
|
10 |
100 |
0 |
0 |
|
32 |
0 |
100 |
0 |
|
56 |
0 |
100 |
0 |
|
56.1 |
0 |
0 |
100 |
| 58 | 0 | 0 | 100 |
| 58.1 | 100 | 0 | 0 |
| 70 | 100 | 0 | 0 |
FLOW RATE: 0.3 mL/min, COLUMN TEMP.: 48 ºC, INJECTION VOLUME: 10 µL OF 0.25 µmole/mL STD.
GARD™
GARD™ Column Protection System
The new GARD™ uses a proprietary material to prevent matrix compounds from passing through (and thereby protecting the analytical column), but allows the analytes of interest to pass unimpeded through the GARD™ and onto the analytical column.
The new GARD™ significantly prolongs column life without band spreading or added pressure. We demonstrated, by means of a performance comparison for Amino Acid Analysis, that the use of a GARD™ will effectively protect the analytical column, will be more cost-effective for the laboratory, is easy to change, is universal to cation-exchange applications, and most importantly has zero band spreading.
Wth the new GARD™ Column protection system from Pickering Laboratories the same cation-exchange GARD™ can be used for nearly all cation-exchange applications.
Pricing & Ordering
Pricing & Ordering
| |||
Catalog # | Description | ||
|---|---|---|---|
1154150 | High-efficiency Sodium Cation-exchange column for protein and collegen hydrosates, 4.0 × 150 mm & 1700-0070 amino acid test mixture | ||
1154150T | Sodium Ion-exchange Column, 4.0 x 150 mm (with test mixture 1700-0070) | ||
1154110T | 30-Minute Sodium Cation-exchange Column 4.6 x 110 mm & 1700-0070 amino acid test mixture | ||
1193250 | Sodium Ion-exchange Column, 3.0 x 250 mm (with test mixture 1700-0070) | ||
| |||
Catalog # | Description | ||
|---|---|---|---|
1700-3102 | Cation-exchange GARD™ assembly: Holder w/ 2 replaceable GARD™ | ||
1700-3101 | Replacement Cation-exchange GARD™ (2/PK) | ||
1700-3100 | GARD™ Holder | ||
