ALKION™ Columns

The stationary phase in the ALKION™ column is a rigid, non-porous polymeric phase that is surface sulfonated. The two modes of separation are ion-exchange and moderate partitioning. The low capacity of the ion-exchange resin makes it an ideal phase for the separation of strongly basic and positively charged compounds. The reversed-phase character allows for discrimination between closely related species.

The unique properties of this column allow for its use in a wide range of applications.

ALKION™ Column Analysis of Biogenic Amines

Post-Column Conditions:

Reagent: 300 mg of OPA, 2 g Thiofluor™, 3 mL of 30 %
Brij® 35 in 950 mL of OD104
Reactor: 45 °C, 0.15 mL
Reagent Flow Rate: 0.3 mL/min

Detection:

Fluorometer: λex 330 nm, λem 465 nm
  

  
  

ALKION™ Column Analysis of Aminoglycoside Antibiotics in Feeds

Post-Column Conditions:

Reagent: 300 mg of OPA, 2g Thiofluor™, 3 mL of 30 % Brij® 35 in 950 mL of OD104
Reactor: 45 °C, 0.15 mL
Reagents flow rate: 0.3 mL/min

Detection:

Fluorometer: λex 330 nm, λem 465 nm

 

  
  

ALKION™ Column Analysis of Streptomycin

Post-Column Conditions:

Reagent 1: 0.75 N Sodium Hydroxide
Reactor 1: ambient, 0.1 mL
Reagent 2: 0.6 % Ninhydrin
Reactor 2: 60 °C, 0.5 mL
Reagents flow rate: 0.3 mL/min

Detection:

Fluorometer: λex 395 nm, λem 500 nm

Or

Reagent 1: Oxidizing Reagent: 100 μL of 5 % Sodium Hypochlorite in GA116
Reactor 1: 50 °C, 0.5 mL
Reagent 2: 300 mg of OPA, 2 g Thiofluor™, 3 mL of 30 % Brij® 35 Solution in 950 mL of OD104
Reactor 2: ambient, 0.1 mL

Detection:

Fluorometer: λex 330 nm, λem 465 nm

 

  
  

GARD™ Column Protection System

The new GARD™ uses a proprietary material to prevent matrix compounds from passing through (and thereby protecting the analytical column), but allows the analytes of interest to pass unimpeded through the GARD™ and onto the analytical column.

The new GARD™ significantly prolongs column life without band spreading or added pressure. We demonstrated, by means of a performance comparison for Amino Acid Analysis, that the use of a GARD™ will effectively protect the analytical column, will be more cost-effective for the laboratory, is easy to change, is universal to cation-exchange applications, and most importantly has zero band spreading.

Wth the new GARD™ Column protection system from Pickering Laboratories the same cation-exchange GARD™ can be used for nearly all cation-exchange applications.

Pricing & Ordering