Overview
AFLACLEAN, OTACLEAN, AFLA-OTACLEAN & DONEX CLEAN-UP COLUMNS
IMMUNOAFFINITY & SPE SAMPLE CLEAN-UP COLUMNS
Aflatoxins and Ochratoxin A are produced by fungi, e.g. Aspergillus and Penicillium species. Therefore both toxin types are found together in many foods and animal feeds, e.g. cereals. Deoxynivalenol, also known as Vomitoxin, is a metabolite of various molds of the genus Fusarium (F. colmorum, F. graminearum) and can often be found together with other mycotoxins.
Of significant assistance is the clean-up of extracts by a combined immunoaffinity column (the Afla-OtaCLEAN column) for both Aflatoxins and Ochratoxin A in one step. The subsequent analysis may then be performed by HPLC with postcolumn derivatization or other techniques.
Afla-OtaCLEAN™
Simultaneous Clean-up of Aflatoxins and Ochratoxin A
The combination sample clean-up column Afla-OtaCLEAN™ from LCTech is very tolerant towards many matrices and allows for a comprehensive clean-up of the aflatoxins B1, B2, G1 and G2 as well as Ochratoxin A. The high maximum capacity of 150 ng for Aflatoxin B1 and 200 ng for Ochratoxin A provides a wide measurement range. With a shelf-life of 18 months (from date of manufacture) at room temperature, the storage and use of the columns is very convenient.
EXAMPLE RECOVERIES OF AFLATOXIN AND OCHRATOXINS
|
MATRIX |
B1 |
B2 |
G1 |
G2 |
OTA |
|---|---|---|---|---|---|
|
Maize (Afla 10 ppb, OTA 14.3 ppb) |
107 % |
91 % |
103 % |
75 % |
97 % |
|
Rice (Afla 10 ppb, OTA 14.3 ppb) |
107 % |
93 % |
98 % |
85 % |
101 % |
|
Malt (Afla 10 ppb, OTA 14.3 ppb) |
98 % |
99 % |
97 % |
70 % |
96 % |
|
Raisins (Afla 10 ppb, OTA 14.3 ppb) |
98 % |
106 % |
101 % |
69 % |
97 % |
Aflatoxin
Sample Clean-up Column for Aflatoxin Analysis
AflaCLEAN™ from LCTech was developed for the sample clean-up of foods, grains, feeds, etc. for aflatoxin analysis using HPLC post-column derivatization or other techniques. The AflaCLEAN™ column is selective for B1, B2, G1 and G2. The maximum loading capacity is 150 ng of Aflatoxin. The shelf-life is 2 years (from date of manufacture) at room temperature. Our cost-effective AflaCLEAN Select columns have a shelf-life of 9 months at 4 ºC.
EXAMPLE RECOVERIES OF AFLATOXIN
|
MATRIX |
B1 |
B2 |
G1 |
G2 |
|---|---|---|---|---|
|
Peanut Butter |
95 % |
98 % |
93 % |
84 % |
|
Peanuts |
104 % |
94 % |
96 % |
85 % |
|
Dried Distillers Grain |
109 % |
97 % |
90 % |
77 % |
|
Maize |
101 % |
98 % |
103 % |
80 % |
Ochratoxin
SAMPLE CLEAN-UP COLUMN FOR OCHRATOXIN ANALYSIS
OtaCLEAN™ was developed for sample clean-up of foods, grains, feeds, etc. for Ochratoxin A analysis using HPLC or other techniques. The antibody employed possesses a very high Ochratoxin A specificity. This leads to exceptional chromatographic results without any interfering secondary signals and very high recovery rates. Independent of the complexity of the matrix, excellent results can be achieved.
The maximum loading capacity is 200 ng Ochratoxin A. The shelf-life is 2 years (from date of manufacture) at room temperature.
EXAMPLE RECOVERIES OF OCHRATOXIN A
|
MATRIX |
RECOVERY RATE |
|---|---|
|
Dried Distillers Grain |
102 % |
|
Duran Wheat |
92 % |
|
Coffee |
100 % |
|
Red Wine |
108 % |
|
Rye Bran |
91 % |
|
Beer |
96 % |
|
Grapes |
91 % |
|
Horse Feed |
94 % |
|
Wort |
107 % |
DONeX
DONeX Sample Clean-up Columns
Deoxynivalenol, also known as Vomitoxin, is a metabolite of various molds of the genus Fusarium (F. culmorum, F. graminearum) and can often be found on contaminated cereals (wheat, barley, oat).
Generally the toxin is analyzed with HPLC/UV detection or alternatively with HPLC / post column derivatization / fluorescence detection or LC / MS. Whichever method is chosen, a good sample preparation extends life time of the analyzer and of the HPLC column and reduces interferences by matrix components. Moreover the running times of the HPLC system can be reduced for easy matrices from about 25 to 10 minutes by pre-cleaning.
If HPLC/UV detection is used, sensitivity can be dramatically enhanced with larger sample volumes.
The DONeX column has a matrix load of up to 4 grams. It is ideally suited to many different matrices, including corn, barley, wheat, animal feeds, muesli, and bread.
DONeX COLUMN RECOVERIES
|
MATRIX |
RECOVERIES [%] DON |
|---|---|
|
Bread |
108 |
|
Corn |
90 |
|
Chicken Feed |
101 |
|
Distillers Grain |
91 |
|
Oat Flacks |
100 |
|
Pasta (dried) |
105 |
|
Poultry Feed |
100 |
|
Rice |
104 |
|
Rye |
91 |
|
Wheat |
100 |
|
Wheat Bran |
99 |
* All matrices were spiked prior to the extraction. Spiking solution was
incubated for at least 1 h with matrix before extraction was started.
Calculation of recovery is based on subtraction of values obtained
without spiking.
Recoveries for Nivalenol are similar to those observed for Deoxynivalenol.
Chromatogram of a cereal-based poultry feed sample,
analyzed with HPLC/post column derivatization/FLD,
sample was spiked with 1 ppm DON, extracted and purified,
evaporated sample (1 g) was resolved in 2 mL HPLC
solvent, 40 μL were injected (0.02 gram matrix equivalents
represent 20 ng DON injected)
ELISA Kits
OtaREAD™ & AflaREAD™
AFLATOXIN AND OCHRATOXIN ELISA TEST KITS
The contamination of food and feed is a worldwide problem. Under inappropriate storage conditions fungi will produce secondary metabolites like mycotoxins, which often render consumption or further processing impossible. As a result of these toxic and hazardous conditions, analyses must be extremely effective, sensitive and reliable to conform to the strict regulations.
The ELISA Test Kits from LCTech meet and exceed all regulatory requirements. The immunological test procedure is based on antibody-antigen recognition and enzymatic color reaction. It leads to a reliable result after a short incubation period and performs quick and sensitive screening of a variety of food products like rice, corn, cocoa, coffee, peanut butter, beer, wine, liquorice and animal feed.
LCTech ELISA kits are delivered ready-to-use and can directly be used for screening sample extracts without further pre-treatment. These kits consist of a shrink-wrapped, coated ELISA plate, the standard solutions, all necessary reagents and a comprehensive manual to guide you through the effective and easy analysis.
FEATURE HIGHLIGHTS
- Reduced costs (no analytical instruments needed)
- Very high sample throughput (96 samples incl. stds. in 130 min)
- Unmatched Aflatoxin B1 sensitivity (0.2 ppb)
- Unmatched Ochratoxin A sensitivity (0.6 ppb for liquids)
- Fast and reliable results
- Highest reproducibility
- Long shelf-life (12 months from date of manufacture)
- Easy handling
- Wide range of matrices including dried distillers grain
- Each kit contains 96-well plate, standards and all necessary solutions
OTAREAD OCHRATOXIN A
The LCTech ELISA kit test can be used with solid liquid matrices. The detection range for solid matrices is 2-20 ppb, for beer 0.6-6 ppb and for wine 0.8-8 ppb.
Pricing & Ordering
Pricing & Ordering
| ||||
Catalog # | Description | Quantity | ||
|---|---|---|---|---|
10514 | AflaCLEAN™, Aflatoxins B1, B2, G1, G2, Pkg 25, 24 month* shelf life at room temp. (Minimum order of 20 packs) | each | ||
12058 | AflaCLEAN™ SELECT, Aflatoxins B1, B2, G1, G2, Pkg 25, 9 month* shelf life at 4 °C (Minimum order of 20 packs) | each | ||
12062 | AflaCLEAN™ SELECT, Aflatoxins B1, B2, G1, G2, Pkg 25, 9 month* shelf life at 4 °C (Minimum order of 20 packs) | each | ||
| ||||
Catalog # | Description | Quantity | ||
|---|---|---|---|---|
12058 | AflaCLEAN™ SELECT, Aflatoxins B1, B2, G1, G2, Pkg 25, 9 month* shelf life at 4 °C (Minimum order of 20 packs) | each | ||
12059 | AflaCLEAN™ SELECT, Aflatoxins B1, B2, G1, G2, Pkg 500, 9 month* shelf life at 4 °C (Minimum order of 4 packs) | each | ||
12062 | AflaCLEAN™ SELECT, Aflatoxins B1, B2, G1, G2, Pkg 25, 9 month* shelf life at 4 °C (Minimum order of 20 packs) | each | ||
12063 | AflaCLEAN™ SELECT, Aflatoxins B1, B2, G1, G2, Pkg 500, 9 month* shelf life at 4 °C (Minimum order of 4 packs) | each | ||
| ||||
Catalog # | Description | Quantity | ||
|---|---|---|---|---|
12862 | AflaCLEAN™ SMART, Aflatoxins B1, B2, G1, G2, Pkg 100, 9 month* shelf life at 4 °C (Minimum order of 8 packs) | each | ||
12863 | AflaCLEAN™ SMART,, Aflatoxins B1, B2, G1, G2, Pkg 1000, 9 month* shelf life at 4 °C (Minimum order of 2 packs) | each | ||
Maximum loading of 100 ng of Aflatoxin. Shelf life is 6 months at 4 ºC.
| ||||
Catalog # | Description | Quantity | ||
|---|---|---|---|---|
10515 | OtaCLEAN™, Ochratoxin A, Pkg 25, 24 month* shelf life at room temp. (Minimum order of 20 packs) | each | ||
11022 | Afla-OtaCLEAN™, Aflatoxins B1, B2, G1, G2 & Ochratoxin A, Pkg 25, 18 month* shelf life at room temp. (Minimum order of 20 packs) | each | ||
11535 | OtaCLEAN™, Ochratoxin A, Pkg 500, 24 month* shelf life at room temp. (Minimum order of 4 packs) | each | ||
12425 | OtaCLEAN™, Ochratoxin A, Pkg 25, 24 month* shelf life at room temp. (Minimum order of 20 packs) | each | ||
12427 | OtaCLEAN™, Ochratoxin A, Pkg 500, 24 month* shelf life at room temp. (Minimum order of 4 packs) | each | ||
| ||||
Catalog # | Description | Quantity | ||
|---|---|---|---|---|
13346 | OtaCLEAN™ SMART, Ochratoxin A, special small format, Pkg 100, 9 month* shelf life at 4 °C (Minimum order of 8 packs) | each | ||
13351 | OtaCLEAN™ SMART, Ochratoxin A, special small format, Pkg 1000, 9 month shelf life* at 4 °C (Minimum order of 2 packs) | each | ||
| ||||
Catalog # | Description | Quantity | ||
|---|---|---|---|---|
12792 | DONeX™, SPE Clean-up Cartridge for DON Analysis, Pkg 25, no expiration at room temp. (Minimum order of 20 packs) | each | ||
12793 | DONeX™, SPE Clean-up Cartridge for DON Analysis, Pkg 500, no expiration at room temp. (Minimum order of 4 packs) | each | ||
