Chromatography Quiz #9 Results
We would like to congratulate the grand prize winner of our last newsletter’s Amino Acids Analysis Chromatography Quiz: Jim Balk from the State of Nebraska DHHS Public Health Environmental Laboratory!!!
He has won, and will shortly be receiving: an Amazon.com gift card! Additionally, all participants will be receiving a gift card from Amazon.com! Again, we would like to thank all of you for your submissions.
The correct answer for the modified Amino Acids chromatogram: the sample has been prepared at the wrong pH. The Aspartic Acid peak is misshapen and splitting, a clear indication that the sample is not sufficiently acidic. After checking the sample pH to determine the required correction, Li220 can be used to adjust to the appropriate pH.
Thank you!
Pickering Labs
Chromatography Quiz #10:
Identify the error made when running the Amino Acids chromatogram below and win a prize! Simply email your answer as well as your full contact information to Rebecca at rlsmith@pickeringlabs.comby August 31st in order to win. You will receive email confirmation that your submission has been received. The troubleshooting answer and winner congratulations will be published in the next issue (to be anonymous, please notify Rebecca in submission).
Hint: Quiz #8 was so popular that we’ve decided to run another hardware problem!
Amino Acid Analysis of Protein Hydrolysate
Pickering Standard: 012506H Protein Hydrolysate Standard, in 0.2 N Sodium citrate buffer pH 2.20, 0.25 µmole/mL, 10 µL injection
Pickering Column: 1193250 Standard Sodium Cation-exchange Column, 3.0 x 250 mm
Normal Operating Conditions: (for reference only, condition changes may be reflected in chromatogram)
Column Temperature: 48 °C
Flow rate: 0.3 mL/min
Eluant Gradient:
|
TIME
|
Na328 %
|
Na740 %
|
RG0011 %
|
|
0
|
100
|
0
|
0
|
|
10
|
100
|
0
|
0
|
|
32
|
0
|
100
|
0
|
|
56
|
0
|
100
|
0
|
|
56.1
|
0
|
0
|
100
|
|
58
|
0
|
0
|
100
|
|
58.1
|
100
|
0
|
0
|
|
70
|
100
|
0
|
0
|
Post-column conditions for amino acid analysis:
Reagent 1: Trione
Reactor 1: 130 °C, 0.5 mL
Reagent flow rate: 0.3 mL/min
Detection: UV-Vis Detector, 570 nm for primary amino acids, 440 nm for secondary amino acids
Chromatogram to Troubleshoot:
Full Chromatogram:
Enlarged View:
Further Enlarged View:
