Chromatography Quiz #35

Chromatography Quiz #34: “What the Brij?”- RESULTS

Pickering Labs would like to congratulate all of our winners for our previous newsletter’s Chromatography Word Puzzle:

Ben Covert from New Mexico State Laboratory Division, Jim Balk from Nebraska Public Health Environmental Lab, Naomi Herrera from New Mexico Department of Health, Joy Gottlieb from New Mexico Department of Health, Josiah Hakala from Minnesota Department of Health, Jiufeng Fan from GSK, Tom Schneider from Suffolk County Water Authority, Narjes Ghafoori from Los Angeles County Public Health Laboratory.

Winners will soon receive: Amazon eGift Card! ($40.00 Value) Happy online shopping!

Congratulations to our quiz winners!

Thank you all for your submissions!

Chromatography Quiz #35 – Bad Carbamate Chromatography

Correctly answer the question below and win a prize! Simply email your answer and your full contact information to Rebecca at by June 15, 2020 in order to win. You will receive email confirmation when your submission is received, and the troubleshooting answer and winner congratulations will be published in the next issue (to be anonymous, please notify Rebecca in submission).

Below you will find the method details for the Carbamate Method. What could be causing the poor peak shape and low response?

Analytical Conditions

          Carbamate Column P/N 0846250
          (250 x 4.6 mm), C8, 5 um
Flow Rate: 
          1 mL/min
Column Temperature: 
          42 ºC
Mobile Phase: 
          see Table 1

Post-column Conditions
Post-column System:
          Pinnacle PCX or Vector PCX
Reactor 1: 
          100 ºC, 0.5 mL
Reactor 2: 
          Ambient, 0.1 mL
Reagent 1: 
          Hydrolysis Reagent CB130 or CB130.2
Reagent 2: 
          100 mg of OPA, 2 g of Thiofluor in
          950 mL of CB910
          FLD, Excitation 330 nm, Emission 465 nm
Injection Volume: 
          10-20 uL

Full method details from our website here.