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CRX-400 has been Discontinued

crx400Dear Valued Customer,

We have discontinued the CRX-400 as of January 1, 2013.  For future sales, our current post-column systems, the Pinnacle PCX and Vector PCX are specifically designed for HPLC post-column applications and will work with any HPLC.

Current CRX-400 users will now need to purchase replacement Vector heated reactors when replacing a clogged or broken heated reactor.  Listed below are the appropriate part numbers for these replacements.

Vector Replacement Heated Reactors:

PN 1452-0160             Reactor Heater and Coil Assembly, 0.15ml, 120V

PN 1452-0161             Reactor Heater and Coil Assembly, 0.15ml, 240V

PN 1452-0162             Reactor Heater and Coil Assembly, 0.5ml, 120V

PN 1452-0163             Reactor Heater and Coil Assembly, 0.5ml, 240V

PN 1452-0325             Reactor Heater and Knitted Assembly, 1.0ml, 120V

PN 1452-0326             Reactor Heater and Knitted Assembly, 1.0ml, 240V

PN 1452-0166             Reactor Heater and Knitted Assembly, 1.4ml, 120V

PN 1452-0167             Reactor Heater and Knitted Assembly, 1.4ml, 240V

PN 1452-0168             Reactor Heater and Knitted Assembly, 2.0ml, 120V

PN 1452-0169             Reactor Heater and Knitted Assembly, 2.0ml, 240V

PN 1452-0170             Reactor Heater and Knitted Assembly, 2.8ml, 120V

PN 1452-0171             Reactor Heater and Knitted Assembly, 2.8ml, 240V

PN 1452-0172             Reactor Heater and Knitted Assembly, 3.0ml, 120V

PN 1452-0173             Reactor Heater and Knitted Assembly, 3.0ml, 240V

PN 1452-0223             Reactor Heater and Knitted Assembly, 3.5ml, 120V

PN 1452-0224             Reactor Heater and Knitted Assembly, 3.5ml, 240V

PN 1452-0174             Reactor Heater and Knitted Assembly, 1.2 & 1.6ml, 120V

PN 1452-0175             Reactor Heater and Knitted Assembly, 1.2 & 1.6ml, 240V

PN 1100-0281             Coil Assembly, 0.5ml without electronics

PN 1100-0283             Knitted Assembly, 1.0ml without electronics

Please contact Technical Support at support@pickeringlabs.com, (800)654-3330, or (650)694-6700 with any questions.

mazawa sig

 

 

David Mazawa

Technical Support Chemist

Pickering exhibits in Las Vegas

-By Mike Gottschalk

Las Vegas, Nevada – Oct. 1 – 5 was the site of the AOAC international show this year.  Pickering Laboratories participated as usual in this important International event. “We see many of our customers and distributors from all over the world at this show” said Wendy Rasmussen, sales manager Pickering Laboratories. “We get to show our new products and catch up with them with new developments in their business.”

Pickering Laboratories presented Posters of our new applications Chrome VI and Antioxidants in foods. The herbal supplement market is a growing market and with FDA guidance, more concerned with testing for quality in their products. “We continue to develop methods in matrices important to this market including the antioxidants, Aflatoxins, Amino Acids and others”  said Laszlo Torma, technical liaison for Pickering Laboratories.

The AOAC international show enables laboratories and suppliers to be involved in the mission of creating analytical methods for today’s most pressing testing challenges. Pickering Laboratories is an award winning member of several committees and laboratory collaborations to further the goal of analytical excellence.

Las Vegas, Nevada – Nov. 6-9 – Yes! Las Vegas Again! This time for the annual Supply Side West show.  1,800 vendors and 30,000 participants meet to network and explore resources in this, the largest gathering of Herbal supplement suppliers and vendors.  Now that the FDA enforcements are increasing, many Herbal supplement manufacturers are working hard to improve their analytical testing capabilities and Pickering Laboratories is there to help.  “With several methods for herbal supplement testing and extensive experience in the food safety industry we can offer experience and laboratory equipment support to the herbal supplement manufactures” – said Mike Gottschalk, VP Marketing, Pickering Laboratories.

Laszlo Torma enjoys the sights and sounds of “Venice”

Pickering Laboratories is a member of the “American Herbal Products Association” and member of the Laboratories Methods Committee for AHPA. The collaboration with AHPA and the Supply Side West show have provided valuable insight to the needs of the Supplement community and the unique capabilities Pickering Laboratories can offer this market.

Glyphosate Use is Increasing

-By Laszlo  Torma

Glyphosate is a commonly used Herbicide worldwide. Here in the United States, it is more commonly known by it’s trade name, RoundUp*.

The South American Crop Protection Monthly Report (August, 2012) predicts strong demand of glyphosate use during the current growing season in Brazil and other South American countries. The combination of aggressive planting of GM soybeans and the increase in planting area of soybean will stimulate the demand for glyphosate use. Brazil is one of the biggest agriculture producers in the world and is also a GM crop production country. In Brazil almost 80% of soybeans are GM soybeans. Roundup Ready soybeans can not only reduce the production cost, but also increase the soybean yield.

What does this mean to the South American analytical laboratories? As the glyphosate use increases, analysis for glyphosate and AMPA likely will increase. Pickering Laboratories analytical method for glyphosate and AMPA in crops is sensitive for low levels of detection and rugged. Sample preparation and cleanup with the cation –exchange column is simplified and the reliable ion exchange post column derivatization analytical protocol can be utilized for overnight runs for increased laboratory production.

For complete method details please visit our website at www.pickeringlabs.com, or click on the link below to download the application note:

MA207_Glyphosate in soy beans

Pickering Laboratories, Inc.
1280 Space Park Way
Mountain  View, CA 94035

Toll-Free: 800-654-3330
Direct: 650-964-6700
Email: sales@pickeringlabs.com

*RoundUp is a trademark of the Monsanto company, www.monsanto.com

 

 

 

The Great One

-By Michael Pickering

One tour of Denali National Park is a bus ride on a dirt and stone road.  The buses are old but so well designed that they are easy to maintain in good working order and well suited to the motorway.  The motorway is in itself a thing of beauty and wonder.  A notch on the flank of a mountain that persists, with some hand maintenance, intact in spite of the onslaught of enormous amounts of erosive rain, snow, and wind.  It is a day replete with photo opportunities, epic vistas, glaciers disguised as mountains, wildlife and flowers, and the lore of Alaska.

Denali Alaska State Flower – the Forget Me Not

When anyone on the bus sees a critter they yell out and the tour guide/bus driver stops and adjusts the position so that all who want a photo can get a good shot.  The grizzlies are much smaller than the famous Kodiaks, as they eat little meat.  They are also blonde, not dark brown, so they are easy to spot at a distance.

Grizzly Crossing Grizzly Bear and her cubs

Denali is the indigenes’ name for Mount McKinley.  It was and is formed by a rising granite pluton that is still growing.  His wife, Mount Foraker, was similarly formed.  All the posters of Mt. McKinley on display had the named taped over and the handwritten DENALI in its place.

Denali National Park and Preserve Denali

When the old world migrants traveled across the land bridge to Alaska, they encountered two main tribes and their languages.  The descendants of these peoples in the lower 48 states are Apaches and Navahos, as evidenced by custom and vocabulary.  Native Alaskans call themselves “sourdoughs,” although all those that I met did not know the origin of the term.  Being a Californian, I of course informed them.  Non-natives can achieve “sourdoughness” by staying for three to four years.  It is sort of a tenure track.  To a person, they are Alaskan.  We, the other 49, are America.  The sourdoughs like to tease Texans (who doesn’t?) by telling them that if Alaska was cut in half, Texas would be the third largest state.  They are also welcoming and generous.  One sensed my interest in plants and spontaneously gave me a recipe for a hypoallergenic flower infusion called “homesteaders honey.”  Of course I will.  

The grandeur of Alaska is enough to draw worldwide tourism, as the languages spoken on our train ride and bus tour can attest.  For us, however, it was our first opportunity to visit our daughter, son-in-law, and grandchildren, who had moved to Anchorage a year earlier.  The train ride across the state and bus tour in the park were our mutual amusement. 

Me (left) and the family Mt. Eielson

The title of this column is “Random Tangents.”  It was assigned to me by the editor, Wendy.  The “T” part I get; I regularly spout them during discussions.  To me, the “T’s” are perfectly germane.  The “R’s” are different though.  The “R” part I suspect is a kindness.  I think it’s the editor cutting me some slack.  So it isn’t a job, like a 500 word essay an assigned topic; an opportunity to be creative and/or amusing.

RANDOM: The eight hour train ride from Anchorage to Fairbanks is lined with antique telephone poles.  Most of the glass insulators, predominantly blue, are still in place.  For that matter, so is much of the wiring. 

TANGENT: It intercepted me ¾ of the way into the bus tour.  The topic was about the cyclic relationship between the snowshoe hares and the bobcats.  They are locked in an approximately seven year cycle of too many and too few.  Those studying the relationship are looking for a correlation factor.  One theory is that it is related to sunshine.  Sun spot/flare activity has a seven year cycle.  I suspect something more prosaic.  The hares preferred food is Willow outer bark.  Willow is the most common bush in the lowlands.  The decline in the hare population is caused by an increasing amount of salicylic acid ingestion.  It weakens the hare, causing attrition and sickens the bobcats alike.

Since the salicylic acid is synthesized only in the inner bark, the Cambrian layer, perhaps the hares engineer their own decline.  After denuding the environment of outer bark, only inner bark is available for forage.  Maybe, exposed to the sun, the Cambrian produces excessive salicylic acid, accelerating the die-off.

 

 

Chromatography Quiz No. 11

Chromatography Quiz #10 Results

We would like to congratulate the grand prize winners of our last newsletter’s Amino Acids Analysis (hardware problem) Chromatography Quiz: Hélène Lachance from Shur-Gain Nutreco in Canada, and Narjes Ghafoori from the LA County Environmental Toxicology Laboratory!!!

They have each won and will be receiving: a Google/Asus Nexus 7 Tablet!  We would like to thank all of you for your submissions.

The correct answer for the modified Amino Acids chromatogram: There was a bubble trapped in the LC Pump head.  Similar noisy baselines could be caused by any of the following: bad pump seals, bad check valves, scratched or damaged pump pistons, faulty pulse dampener, or problems with connections to the pump.  Post-column systems containing reciprocating pumps can also cause noisy pulsations in the baseline, and the frequency of the sine waves should be evaluated to determine which pump is causing the noise. 

This problem can also be caused by dirty reagents or eluents, however in this case the pulsations would be accompanied by the elevation of the background signal. 

Thank you!
Pickering Labs

Chromatography Quiz #11:

Identify the error made when running the Glyphosate chromatogram below and win a prize!  Simply email your answer as well as your full contact information to Rebecca at rlsmith@pickeringlabs.com by January 31, 2013 in order to win.  You will receive email confirmation that your submission has been received.  The troubleshooting answer and winner congratulations will be published in the next issue (to be anonymous, please notify Rebecca in submission). 

Glyphosate Analysis for US EPA Method 547

Pickering Standard: Glyphosate Test Mixture 1700-0080, 2.5 ug/mL, 10 µL injection 

Pickering Column: 1954150 Cation-exchange Column for Glyphosate Analysis,
4 x 150 mm

Normal Operating Conditions: (for reference only, condition changes may be reflected in chromatogram)

Column Temperature: 55 °C

Flow rate: 0.4 mL/min

Eluent Gradient:

TIME K200 RG019 %
0 100 0
15 100 0
15.1 0 100
17 0 100
17.1 100 0
27 100 0

Post-column conditions for pesticide analysis:

Reagent 1: Oxidizing reagent – 100uL of 5% Sodium Hypochlorite in 950mL of GA116

Reagent 2: 100 mg of OPA, 2 g Thiofluor™ in 950 mL of GA104

Reactor 1: 36 °C, 0.5 mL

Reactor 2: ambient. 0.1 mL

Reagent flow rates: 0.3 mL/min

Detection: Fluorometer ex 330 nm, em 465 nm

 

Note: A special thanks to Michael Pickering for the suggestion and for providing the “old school” chromatograms (notice the chart reader font on the retention times)!

                                    

 

Glyphosate Guard column to be replaced by New GARD

In an effort to offer superior products, Pickering Laboratories will replace the Glyphosate Guard column (PN 1953020) with the new GARD column protection system <link to GARD abstract>. The Glyphosate Guard Column (PN 1953020) will no longer be available starting Januray 1, 2013. The new GARD prolongs column life without additional back pressure or band spreading. The GARD is an easily replaceable cartridge, which should be replaced every 3 months or 300 injections. Please contact Technical Support with any questions.
.
PN 1700-3102    Cation-exchange GARD assembly: includes holder and 2 replaceable GARDs
PN 1700-3101    Replacement cation-exchange GARDs (2/pack)
PN 1700-3100    GARD Holder
.
Regards,
David Mazawa
Technical Support Chemist
Pickering Laboratories, Inc.
1280 Space Park Way
Mountain View, CA 94043
Phone: 650-694-6700 ext 710
Fax: 650-968-0749
Email: david.mazawa@pickeringlabs.com

Midwest AOAC Meeting

This year’s Midwest AOAC meeting was held in St. Louis, Missouri from June 4-6. 
It was well-attended with a good technical program.

Gateway Arch in St. Louis, MO*

At this year’s meeting we hosted an Amino Acid workshop, in which attendees learned tips and troubleshooting techniques for amino acid analysis. All analytical methods were covered, including some discussions on sample preparation for amino acids.

The post-column analysis of amino acids is a tried and true technique. By derivatizing the amino acids post-column, any matrix components which might otherwise interfere with the derivatization steps are eliminated, leaving only the amino acids to react with the reagents. This leads to exceptional reproducibility, sensitivity and ruggedness.

Our Pinnacle PCX instrument is especially suited to amino acid analysis with its heated column oven (to perform temperature gradients), and pulse-free syringe pumps. More information can be found on our website: www.pickeringlabs.com, or by downloading one of the following:
MA380:  30-Minute Amino Acid Analysis of Hydrolyzed Samples
or our Amino Acid Brochure
View of St. Louis from the top of the Arch

* Interesting Fact: Gateway Arch is part of the Jefferson National Expansion Memorial. It was designed in 1947, but construction did not begin until 1965.  It opened to the public in 1967. http://en.wikipedia.org/wiki/Gateway_Arch

Guaranteed Chemistry