Category Archives: ochratoxin

AOAC International Meeting

fountainThe AOAC International held their annual meeting in Chicago, Illinois from August 25-27, 2013.

Pickering Laboratories were on hand to share our latest methods and instruments.  We also gave an Exhibitor Presentation titled “An Automated Highly Sensitive Method for Aflatoxin B/G or Ochratoxin A Clean-up and Analysis – A Novel Approach in Mycotoxin Analysis”

The purpose of the talk was to showcase our newest method and instrument:

Building on the FREESTYLE Automated Sample Preparation Instrument, the new ThermELUTE module and columns enable rapid, sensitive analysis of Aflatoxin or Ochratoxin A. The ThermELUTE module uses a thermal denaturation technique to release jellybean4toxin from a new column format requiring only 20% of usual solvent volumes, and FREESTYLE quantitatively transfers the eluate into the HPLC. In combination with online injection, analytical sensitivity can be increased without manual manipulation. The increase in sensitivity provides a reduction of sample load, while maintaining excellent chromatographic results. The increase of sensitivity allow analysis of mycotoxin levels well below regulatory limits.

The ThermELUTE takes the separate steps of cleanup and analysis, and combines them into one automated system. Using a FREESTYLE robotic system equipped with the

FREESTYLE Automated Sample Preparation System
FREESTYLE Automated Sample Preparation System

ThermELUTE module, we can now clean up a sample for mycotoxins, and then directly inject the cleaned sample onto the HPLC column for analysis!

Automating Mycotoxin Analyses from Cleanup to Detection:

  • Results 3 times faster
  • 80% less solvent
  • Direct connect to HPLC

The brochure for ThermELUTE can be found HERE. To view the slides from our presentation, click HERE.

 

Pickering Laboratories in 2013

By Wendy Rasmussen

In recent years, we have released several new products and applications, and with still more on the horizon, it occurred to me that now would be a great time to summarize the Pickering of today – our mindset and our wide variety of products & applications.

Acai Berries
Acai Berries

No longer are we simply the “Post-Column Company”. We are the “Automated-Sample Antioxidants” company.  Think of us as the new “super fruit.” The Acai berry, or perhaps the new Chia Seeds (incidentally, we do have a post-column application for the identification & quantitation of  Antioxidants in a variety of matrices).

We are still very active, and we as a company plan to be here for many years to come. We are still the company founded on chemistry and a desire to to teach, to spread our technical expertise, to support our customers.

It’s been a few years now since Pickering began distributing and supporting our LCTech Product line.  The products have shown an ever increasing interest here in the US and in Canada (our official Sales Territory for this product line). We are very proud to offer these products and we hope we can develop it further in the future.

Historically, we have provided the back-end of an analysis (post-column derivatization). Nowadays, we can provide the front-end of analysis as well (the sample-cleanup).

In thinking about our product offerings, I realized that a simple list does not effectively show the scope of the products we have to offer in 2013 – primarily because we have a lot of overlap between products and product lines.  We’re not a vertical company in that regard.  I suppose one could say our product offerings are more circular in that many do not fall into a single distinct category.  I am a very visual person, and for me, a Venn diagram and our overall “product scale” really helped to understand and clarify our products:

Venn Diagram of Pickering Laboratories
Venn Diagram of Pickering Laboratories
General "Product Scale" for Pickering Offerings
General “Product Scale” for Pickering Offerings
For those of who like lists, you can find one Here, on Pickering’s website, and on LCTech’s Website

For any Questions, please feel free to contact us:

Pickering Laboratories, Inc.
Mountain View, California
Email: sales@pickeringlabs.com
Phone: (direct) 650-694-6700 or (toll-free) 800-654-3330

 

Image of Acai Berries:  http://acaiberryeducation.com/

Pickering Exhibits at Pittcon 2013

This year’s Pittcon was held in Philadelphia, Pennsylvania from March 17-21, 2013.

view of philly from the museum On display were Pinnacle PCX, EluVAC, and a video of the FREESTYLE Automated Sample Preparation Instrument.  The video was a new technique for us, and it was well received.

The video in our booth was a combination of the separate videos for each module of the FREESTYLE Automated Sample Preparation Instrument. You can view the originals here (they are very informative, and we highly recommend them): FREESTYLE VIDEOS

Wendy, Mike and David enjoyed visiting with existing and future customers, as well as our distributors from around the world, including our Canadian distributor, Chromatographic Specialties.

Before the show opened, we had the opportunity to explore the city.  Philadelphia is one of our oldest cities and is full of history and landmarks. Some of this editor’s favorites include Independence Hall and the surrounding neighborhoods, and the Art Museum (see more below).  On this trip, we explored Reading Terminal Market, and sampled that famous cuisine, the Philly Cheese steak Sandwich. I’m told it is not truly a Philly Steak sandwich unless you have it with Cheez Whiz. Any type of real cheese doesn’t count. I made that mistake on my first trip to Philadelphia, and the man behind the counter let me know it!  I wasn’t about to make that mistake again:

philly cheesesteak

The Philadelphia Museum of Art is known for its amazing collection of artwork (viewed on a previous visit), but is also made famous by the scene of Sylvester Stallone running up the stairs in the movie Rocky.  Since the author of this entry happened to be training for a race, she decided that a little cold wasn’t going to stop her. So she put on her iPod (Rocky Theme playing, of course), and ran from the hotel to the top of the steps and back (total distance: about 2.8miles).

view from the bottom

wendy philly top of steps

wendy rockyOn the return to the hotel, I came across a version of the LOVE sculpture, by American Artist Robert Indiana. I always thought it was was much bigger…

love sculpture

 

For those of you interested in learning more about the sculpture, click HERE

If you’re ever in Philadelphia, the Philadelphia Museum of Art is definitely worth a visit.

But if you can’t make the trip to Philadelphia, you could also stay home and watch Rocky…

On-Demand Webinar: Rugged and Sensitive Analysis of Mycotoxins in Food Matrices

If you missed the live webinar last summer, you can still hear the Webinar On-Demand! Just click on the links below to launch the presentation.
On August 14th, 2012, in collaboration with Thermo Scientific, Pickering Laboratories participated in a free webinar titled: Rugged and Sensitive Analysis of Mycotoxins in Food Matrices
 
The webinar was hosted by LC/GC Magazine and Thermo Scientific.
 
Live Webcast: Tuesday, August, 14, 2012 at 8:00 am PDT; 11:00 am EDT; 15:00 GMT

Register Free at http://www.chromatographyonline.com/foodmatrices
EVENT OVERVIEW
Mycotoxins are found in a staggering range of matrices, from grains to fruits, spices and nuts to animal feed. They are of major concern for their negative impact on the worldwide economy from product revenue losses, their toxicity to animals and humans, and their persistence throughout multiple food processing steps.Learn how Pickering Laboratories addresses this challenge and meets guidelines set by the FDA using sensitive and rugged Thermo Scientific UHPLC methods to effectively detect Aflatoxins as well as other key classes of Mycotoxins. These easy-to-use methods utilize immunoaffinity clean-up and post-column derivatization.

Also join us to discover the benefits of a Thermo Scientific LC-MS/MS method using automated Turbulent Flow sample extraction to separate Mycotoxins from complex food matrices. A highly efficient solution used in high throughput laboratories that eliminates manual sample preparation, improves method reliability and drives down cost per sample.

Who Should Attend
Analytical Chemists
Lab Managers
Food Science Researchers
Presenters
Wendy E. Rasmussen
Wendy E. Rasmussen
Technical Expert
Food, Agricultural and Environmental Markets
Pickering Laboratories
Markus M. Martin
Markus M. Martin
Solutions Manager LC/MS
Thermo Fisher Scientific
Dr. Yang Shi
Dr. Yang Shi
Senior Applications Scientist
LC/MS Applications
Thermo Fisher Scientific
Laura Bush
Moderator:
Laura Bush
Editorial Director
LCGC North America
Presented by
Sponsored by
LC/GC Logo
Thermo Logo
 

Pickering Products

Mycotoxins: Clean-up Columns, ELISA Kits, Post-Column Derivatization Instruments & Methods, SPE manifolds (manual & automated) for the analysis of:

Aflatoxins
Ochratoxin A
DON
Nivalenol
Fumonisin FB1, FB2
Zearalenone
Ergot Alkaloids

Post-Column Derivatization: Pinnacle PCX, Vector PCX, UVE Photochemical Reactor, Columns, Reagents, Eluants for the analysis of:

Antioxidants
Mycotoxins (individual and multi-residue)
Amino Acids
Biogenic Amines
Paralytic Shellfish Toxin
Polyether Antibiotics
Hexavalent Chromium
Vitamins
Sugars
Nitrate/Nitrite
Bromate
Carbamate Pesticides
Glyphosate Herbicide
NDELA
Formaldehyde

Sample Preparation (clean-up) Instruments:

FREESTYLE for SPE, GPC, online-concentration
FREESTYLE ThermELUTE for Aflatoxin analysis (direct inject onto HPLC)
AcceCLEAN for SPE (incl. Immunoaffinity columns)
EluVAC vacuum manifold for SPE (incl. Immunoaffinity columns)
GPC QUATTRO for manual GPC Cleanup
DECS System for the cleanup of samples for Dioxin analysis

Chemistry Products:

Artificial Perspiration
Artificial Saliva
High Purity Water

Stand-Alone OEM Syringe Pump:

For any applications requiring an inert flow path, volumes up to 70mL, and pressures up to 500psi

For More Information visit:

www.pickeringlabs.com

or email: sales@pickeringlabs.com

New Product Lines from Pickering, October 2009

Mycotoxin product line
We are now distributing Mycotoxin Immunoaffinity products for Ochratoxin and Aflatoxin. The performance and batch-to-batch reproducibility of the columns is exceptional and far exceeds that of other manufacturers. The columns can be used for any matrix, from wine and juice, to nuts and grains, to herbs and spices. Contact Sales for more information.

GPC Sample Clean up line
We have a new GPC Sample Clean-up product line! We have both automated and manual GPC cleanup systems and we also have systems that include concentration & solvent exchange, or just GPC. Sample cleanup using GPC is especially useful for fatty matrices, but also perfect for vegetable matter and spices, as well as soil & waste water.

New faster AAA columns
We now have a Lithium amino acid run which will separate 45 amino acids in 70 minutes for Physiologic fluids, an a new 30-min Sodium amino acid run which will separate the 20 amino acids commonly found in protein hydrolysate samples. These columns are for use with our Pinnacle PCX.

Histamine Product Line
Our newly launched Histamine product line consists of Dip-sticks and Elisa kits as well as Post-column derivatization for fast and in-situ testing as well as quick, reproducible, and sensitive methods for follow-up confirmation. Contact Pickering Laboratories at 1-800-654-3330 or sales@pickeringlabs.com for more information!