Category Archives: Physiological Fluids

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Pickering Laboratories in 2013

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By Wendy Rasmussen

In recent years, we have released several new products and applications, and with still more on the horizon, it occurred to me that now would be a great time to summarize the Pickering of today – our mindset and our wide variety of products & applications.

Acai Berries
Acai Berries

No longer are we simply the “Post-Column Company”. We are the “Automated-Sample Antioxidants” company.  Think of us as the new “super fruit.” The Acai berry, or perhaps the new Chia Seeds (incidentally, we do have a post-column application for the identification & quantitation of  Antioxidants in a variety of matrices).

We are still very active, and we as a company plan to be here for many years to come. We are still the company founded on chemistry and a desire to to teach, to spread our technical expertise, to support our customers.

It’s been a few years now since Pickering began distributing and supporting our LCTech Product line.  The products have shown an ever increasing interest here in the US and in Canada (our official Sales Territory for this product line). We are very proud to offer these products and we hope we can develop it further in the future.

Historically, we have provided the back-end of an analysis (post-column derivatization). Nowadays, we can provide the front-end of analysis as well (the sample-cleanup).

In thinking about our product offerings, I realized that a simple list does not effectively show the scope of the products we have to offer in 2013 – primarily because we have a lot of overlap between products and product lines.  We’re not a vertical company in that regard.  I suppose one could say our product offerings are more circular in that many do not fall into a single distinct category.  I am a very visual person, and for me, a Venn diagram and our overall “product scale” really helped to understand and clarify our products:

Venn Diagram of Pickering Laboratories
Venn Diagram of Pickering Laboratories
General "Product Scale" for Pickering Offerings
General “Product Scale” for Pickering Offerings
For those of who like lists, you can find one Here, on Pickering’s website, and on LCTech’s Website

For any Questions, please feel free to contact us:

Pickering Laboratories, Inc.
Mountain View, California
Email: sales@pickeringlabs.com
Phone: (direct) 650-694-6700 or (toll-free) 800-654-3330

 

Image of Acai Berries:  http://acaiberryeducation.com/

Amino Acid Analysis, Chromatography, Chromatography Quiz, Physiological Fluids, Post-column

Chromatography Quiz No. 2

Congratulations to the winners of our last newsletter’s Chromatography Quiz: Matthew Hartz, Jamie Palmer, and Keena Njoroge from Underwriters Laboratories, Sudheer Reddy from Chemtex, and Becky Canela from Environmental Laboratory Services!

two dozen irresistible cookiesThey’ve each won, and will shortly be receiving from Gifttree.com, two dozen irresistible cookies in five flavors: White Chocolate Hazelnut, Snickerdoodle, Peanut Butter, Oatmeal Raisin, and Chocolate Chip.

The correct answer for the modified Carbamates chromatogram: we reversed the two reagents. The OPA reagent was pumped in the Reagent One position, and the Hydrolysis reagent was pumped in the Reagent Two position. Thus 1-Naphthol, which is naturally fluorescent, appears full-sized. The other Carbamate peaks have different sizes due to their varying rate of hydrolysis – the high pH of the OPA reagent will allow for some but not complete hydrolysis prior to detection.

Chromatography Quiz: Amino Acid Analysis

Identify the error made when running the Amino Acids chromatogram below and win a prize! Simply email your answer and your full contact information to Rebecca at rlsmith@pickeringlabs.com by March 1st in order to win. The troubleshooting answer and winner congratulations will be published in the next issue (to be anonymous, please notify Rebecca in submission).

Amino Acid Analysis of Physiological Fluids:

Pickering Standard: 011006P Native Sample Standard 0.25 µmole/mL, 10 µL injection

Pickering Column: 0354100T High Efficiency Lithium Cation-exchange Column, 4.0 x 100 mm

Normal Operating Conditions: (for reference only, condition changes may be reflected in chromatogram)

Column Temperature: 36 °C

Flow rate: 0.35 mL/min

Eluent Gradient:
Amino Acid Analysis of Physiological Fluids

Post-column conditions for amino acid analysis:
Reagent 1: Trione
Reactor 1: 130 °C, 0.5 mL
Reagent flow rate: 0.3 mL/min

Detection: UV-Vis Detector, 570nm for primary amino acids, 440nm for secondary amino acids UV-Vis Detector
Hint: Assume in this case that both Guard and Analytical column are good. For more information, search standard Amino Acid chromatogram.