Category Archives: post-column derivatization

parts, post-column derivatization

CRX-400 has been Discontinued

crx400Dear Valued Customer,

We have discontinued the CRX-400 as of January 1, 2013.  For future sales, our current post-column systems, the Pinnacle PCX and Vector PCX are specifically designed for HPLC post-column applications and will work with any HPLC.

Current CRX-400 users will now need to purchase replacement Vector heated reactors when replacing a clogged or broken heated reactor.  Listed below are the appropriate part numbers for these replacements.

Vector Replacement Heated Reactors:

PN 1452-0160             Reactor Heater and Coil Assembly, 0.15ml, 120V

PN 1452-0161             Reactor Heater and Coil Assembly, 0.15ml, 240V

PN 1452-0162             Reactor Heater and Coil Assembly, 0.5ml, 120V

PN 1452-0163             Reactor Heater and Coil Assembly, 0.5ml, 240V

PN 1452-0325             Reactor Heater and Knitted Assembly, 1.0ml, 120V

PN 1452-0326             Reactor Heater and Knitted Assembly, 1.0ml, 240V

PN 1452-0166             Reactor Heater and Knitted Assembly, 1.4ml, 120V

PN 1452-0167             Reactor Heater and Knitted Assembly, 1.4ml, 240V

PN 1452-0168             Reactor Heater and Knitted Assembly, 2.0ml, 120V

PN 1452-0169             Reactor Heater and Knitted Assembly, 2.0ml, 240V

PN 1452-0170             Reactor Heater and Knitted Assembly, 2.8ml, 120V

PN 1452-0171             Reactor Heater and Knitted Assembly, 2.8ml, 240V

PN 1452-0172             Reactor Heater and Knitted Assembly, 3.0ml, 120V

PN 1452-0173             Reactor Heater and Knitted Assembly, 3.0ml, 240V

PN 1452-0223             Reactor Heater and Knitted Assembly, 3.5ml, 120V

PN 1452-0224             Reactor Heater and Knitted Assembly, 3.5ml, 240V

PN 1452-0174             Reactor Heater and Knitted Assembly, 1.2 & 1.6ml, 120V

PN 1452-0175             Reactor Heater and Knitted Assembly, 1.2 & 1.6ml, 240V

PN 1100-0281             Coil Assembly, 0.5ml without electronics

PN 1100-0283             Knitted Assembly, 1.0ml without electronics

Please contact Technical Support at support@pickeringlabs.com, (800)654-3330, or (650)694-6700 with any questions.

mazawa sig

 

 

David Mazawa

Technical Support Chemist

glyphosate, post-column derivatization

Glyphosate Use is Increasing

-By Laszlo  Torma

Glyphosate is a commonly used Herbicide worldwide. Here in the United States, it is more commonly known by it’s trade name, RoundUp*.

The South American Crop Protection Monthly Report (August, 2012) predicts strong demand of glyphosate use during the current growing season in Brazil and other South American countries. The combination of aggressive planting of GM soybeans and the increase in planting area of soybean will stimulate the demand for glyphosate use. Brazil is one of the biggest agriculture producers in the world and is also a GM crop production country. In Brazil almost 80% of soybeans are GM soybeans. Roundup Ready soybeans can not only reduce the production cost, but also increase the soybean yield.

What does this mean to the South American analytical laboratories? As the glyphosate use increases, analysis for glyphosate and AMPA likely will increase. Pickering Laboratories analytical method for glyphosate and AMPA in crops is sensitive for low levels of detection and rugged. Sample preparation and cleanup with the cation –exchange column is simplified and the reliable ion exchange post column derivatization analytical protocol can be utilized for overnight runs for increased laboratory production.

For complete method details please visit our website at www.pickeringlabs.com, or click on the link below to download the application note:

MA207_Glyphosate in soy beans

Pickering Laboratories, Inc.
1280 Space Park Way
Mountain  View, CA 94035

Toll-Free: 800-654-3330
Direct: 650-964-6700
Email: sales@pickeringlabs.com

*RoundUp is a trademark of the Monsanto company, www.monsanto.com

 

 

 

aflatoxin, deoxynivalenol, DON, HPLC, immunoaffinity cleanup, Multi-residue Mycotoxin Analysis, mycotoxin, ochratoxin, pinnacle pcx, post-column derivatization, UVE, vomitoxin

On-Demand Webinar: Rugged and Sensitive Analysis of Mycotoxins in Food Matrices

If you missed the live webinar last summer, you can still hear the Webinar On-Demand! Just click on the links below to launch the presentation.
On August 14th, 2012, in collaboration with Thermo Scientific, Pickering Laboratories participated in a free webinar titled: Rugged and Sensitive Analysis of Mycotoxins in Food Matrices
 
The webinar was hosted by LC/GC Magazine and Thermo Scientific.
 
Live Webcast: Tuesday, August, 14, 2012 at 8:00 am PDT; 11:00 am EDT; 15:00 GMT

Register Free at http://www.chromatographyonline.com/foodmatrices
EVENT OVERVIEW
Mycotoxins are found in a staggering range of matrices, from grains to fruits, spices and nuts to animal feed. They are of major concern for their negative impact on the worldwide economy from product revenue losses, their toxicity to animals and humans, and their persistence throughout multiple food processing steps.Learn how Pickering Laboratories addresses this challenge and meets guidelines set by the FDA using sensitive and rugged Thermo Scientific UHPLC methods to effectively detect Aflatoxins as well as other key classes of Mycotoxins. These easy-to-use methods utilize immunoaffinity clean-up and post-column derivatization.

Also join us to discover the benefits of a Thermo Scientific LC-MS/MS method using automated Turbulent Flow sample extraction to separate Mycotoxins from complex food matrices. A highly efficient solution used in high throughput laboratories that eliminates manual sample preparation, improves method reliability and drives down cost per sample.

Who Should Attend
•
Analytical Chemists
•
Lab Managers
•
Food Science Researchers
Presenters
Wendy E. Rasmussen
Wendy E. Rasmussen
Technical Expert
Food, Agricultural and Environmental Markets
Pickering Laboratories
Markus M. Martin
Markus M. Martin
Solutions Manager LC/MS
Thermo Fisher Scientific
Dr. Yang Shi
Dr. Yang Shi
Senior Applications Scientist
LC/MS Applications
Thermo Fisher Scientific
Laura Bush
Moderator:
Laura Bush
Editorial Director
LCGC North America
Presented by
Sponsored by
LC/GC Logo
Thermo Logo
 
aflatoxin, deoxynivalenol, DON, eluvac, freestyle, immunoaffinity cleanup, mycotoxin, post-column derivatization, UVE, vomitoxin

New Mycotoxin Cleanup Columns!

2 Comments
Also on display this year at Pittcon were the a couple of NEW! Mycotoxin clean-up columns available from Pickering Laboratories:
AflaCLEAN SMART
The immunoaffinity column AflaCLEAN SMART is designed for sample clean-up for subsequent Aflatoxin analysis. 
This columns is smaller than the other counterparts on the market – it uses only a fraction of the solvent and yet has 100ng loading capacity as specified by the AOAC methods. 
During extraction, dilution, washing, sample loading and elution, more than 80% of the solvents can be saved.

The final elution volume is only 400uL instead of the typical 2mL – and yet has the same final concentration as with other columns.  As most methods inject only 20-50uL of sample onto the autosampler, this volume is more than enough for analysis. 

These columns can also be used with the very convenient and affordable EluVac vacuum manifold.

If you would like additional information, please email us: sales@pickeringlabs.com

DONeX
The DONeX column can be used in the sample preparation for the analysis of the mycotoxin Deoxynivalenol (Vomitoxin), which is often found on contaminated cereals and grains such as corn. 
The column is ideally suited to many common matrices such as corn, barley, oat, wheat, rye or cereal-based feed. The advantage of DONex is that these columns can also be used with complex matrices and one can stll obtain excellent recovery rates. 
DONeX is used with a sample load of 1 g for HPLC / post column / fluorescence detection or LC / MS.  By increasing the matrix load up to 4 g on column, users with HPLC / UV detection systems can reach detection limits as low as 200 ppb. 
Unlike our other Mycotoxin clean-up columns, DONeX is not an immunoaffinity column, and so it can be stored on the shelf and has an undefined shelf-life.
The DONeX clean-up column is based on a 3mL standard form and thus perfectly suitable for automated handling in the LCTech systems AcceCLEANTM and FREESTLYETM SPE. 
If you are interested in any additional information or would like to try a sample, please send us an email: sales@pickeringlabs.com
5200, pinnacle, pinnacle pcx, post-column derivatization, vector

Obsolescence of PCX 5200 in February 2013

Special Note! The Pinnacle PCX and  Vector PCX instruments are available and will continue to be 100% supported for the foreseeable future!

The PCX 5200 will no longer be supported starting February 2013. This means we will no longer keep replacement parts in stock starting February 2013.  

PCX5200 will be obsoleted in February 2013

Common parts like tubing, nuts, ferrules, and filters that are used on the Pinnacle and the Vector will still be available.

Our current models, the Pinnacle PCX and Vector PCX are a great upgrade option to the PCX 5200. You can download our PCX Product Brochure from the Pickering website or find more information by clicking on the individual links.

If you have any questions, please contact support@pickeringlabs.com 

If you would like a quotation for a new system or to place an order, please contact orders@pickeringlabs.com 

David Mazawa,Technical Support Chemist

AcceCLEAN, aflatoxin, Amino Acid Analysis, amino acids, antioxidants, artificial perspiration, carbamate, clean-up, deoxynivalenol, dietary supplements, elisa, eluvac, ergot alkaloid, freestyle, glyphosate, GPC, immunoaffinity cleanup, Multi-residue Mycotoxin Analysis, mycotoxin, natural toxins, Ninhydrin, ochratoxin, paralytic shellfish toxin, pinnacle pcx, post-column derivatization, psp, syringe pump, Trione, UVE, vomitoxin

Pickering Products

1 Comment

Mycotoxins: Clean-up Columns, ELISA Kits, Post-Column Derivatization Instruments & Methods, SPE manifolds (manual & automated) for the analysis of:

Aflatoxins
Ochratoxin A
DON
Nivalenol
Fumonisin FB1, FB2
Zearalenone
Ergot Alkaloids

Post-Column Derivatization: Pinnacle PCX, Vector PCX, UVE Photochemical Reactor, Columns, Reagents, Eluants for the analysis of:

Antioxidants
Mycotoxins (individual and multi-residue)
Amino Acids
Biogenic Amines
Paralytic Shellfish Toxin
Polyether Antibiotics
Hexavalent Chromium
Vitamins
Sugars
Nitrate/Nitrite
Bromate
Carbamate Pesticides
Glyphosate Herbicide
NDELA
Formaldehyde

Sample Preparation (clean-up) Instruments:

FREESTYLE for SPE, GPC, online-concentration
FREESTYLE ThermELUTE for Aflatoxin analysis (direct inject onto HPLC)
AcceCLEAN for SPE (incl. Immunoaffinity columns)
EluVAC vacuum manifold for SPE (incl. Immunoaffinity columns)
GPC QUATTRO for manual GPC Cleanup
DECS System for the cleanup of samples for Dioxin analysis

Chemistry Products:

Artificial Perspiration
Artificial Saliva
High Purity Water

Stand-Alone OEM Syringe Pump:

For any applications requiring an inert flow path, volumes up to 70mL, and pressures up to 500psi

For More Information visit:

www.pickeringlabs.com

or email: sales@pickeringlabs.com

antioxidants, post-column derivatization

Antioxidants Are No Accident

Recently we learned about a paper published by a team in Poland describing their work on the analysis of anti-oxidants. We wanted to share this work with the AHPA community.
Barbara Kusznierewicz and her colleagues from Gdansk University of Technology have developed a method to analyze antioxidants in complex mixtures by HPLC with post-column derivatization and UV/Vis detection. The authors studied three well-known reagents routinely used in colorimetric assays to measure antioxidant activity. They determined that Diammonium 2,2′-azinobis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and Folin-Ciocalteu reagent (FCR) were best suited for post-column derivatization of antioxidants after they were separated on reversed-phase HPLC column.
Photo from FoodProductDesign.com

Researches optimized HPLC conditions as well as post-column parameters such as concentration of post-column reagent, duration of derivatization reaction and reaction temperature. Trolox and 20 other phenolic compounds were investigated and scientists were able to demonstrate good correlation between Trolox equivalent antioxidant capacity (TEAC) determined by HPLC with post-column derivatization and standard colorimetic assay. This method was successfully applied to analysis of extracts from sloe fruits and mirabelle plums and allowed to calculate concentration of individual antioxidants as well as TE antioxidant capacity.


Pickering Laboratories strives to provide new post-column applications, and the instruments and chemistry to support these applications. It is our philosophy to provide a complete solution and customer support. We plan to follow-up this interesting research with some development of our own to see if we can optimize the post-column technique further. 
The full version of the paper can be found in the Wiley online library:
REFERENCE:
Kusznierewicz, B., Piasek, A., Bartoszek, A., Namiesnik, J., “The Optimisation of Analytical Parameters for Routine Profiling of Antioxidants in Complex Mixtures by HPLC Coupled Post-column Derivatization,” Phytochemical Analysis, 22, 392-402 (2011)