Chromatography Quiz #12 Results
We would like to congratulate our grand prize winners of our last newsletter’s Amino Acid Analysis Chromatography Quiz: Luiz Paulo Mousinho from Chemetric in Brazil, Helene Lachance from Shur-Gain Nutreco in Canada, and Narjes Ghafoori from the LA County Environmental Toxicology Laboratory!!!
They have each won and will be receiving: a $100 gift card from Amazon.com! We would like to thank all of you for your submissions.
The correct answer for the modified Amino Acids chromatogram: the back pressure regulator on the outlet of the detector stopped functioning properly. This was indeed another hardware troubleshooting quiz! The pattern of noise seen in the customer’s baseline is characteristic to this problem, and a new back pressure regulator eliminated the noise.
Thank you!
Pickering Labs
Chromatography Quiz #13:
Identify the error made when running the Carbamate chromatogram below and win a prize! Simply email your answer as well as your full contact information to Rebecca at rlsmith@pickeringlabs.com by August 30, 2013 in order to win. You will receive email confirmation that your submission has been received. The troubleshooting answer and winner congratulations will be published in the next issue (to be anonymous, please notify Rebecca in submission).
Carbamate Analysis for US EPA Method 531.1
Pickering Standard: 1700-0063 Carbamate Test Mixture, 2.5 µg/mL, 10 µL injection
Pickering Column: 1846250 Carbamate Column, C18, 4.6 x 250 mm
Normal Operating Conditions: (for reference only, condition changes may be reflected in chromatogram)
Column Temperature: 42 °C
Flow rate: 1 mL/min
Eluent Gradient:
|
TIME |
WATER |
MeOH % |
|
0 |
85 |
15 |
|
1 |
85 |
15 |
|
44 |
25 |
75 |
|
44.1 |
0 |
100 |
|
49 |
0 |
100 |
|
49.1 |
85 |
15 |
|
57 |
85 |
15 |
Post-column conditions:
Reagent 1: Hydrolysis reagent CB130
Reagent 2: 100 mg of OPA, 2 g Thiofluor™ in 950 mL of CB910
Reactor 1: 100 °C, 0.5 mL
Reactor 2: ambient. 0.1 mL
Reagent flow rates: 0.3 mL/min
Detection: Fluorometer ex 330 nm, em 465 nm
Troubleshooting Chromatogram:
Reference Chromatogram:
