All posts by Pickering Laboratories

maintenance, pickering laboratories, pinnacle, pinnacle pcx, Post-column, post-column derivatization, Troubleshooting

Replacing the Over-pressure Relief Valve Cartridge

Mixing-Manifold-1a 
Cleaning and Reassembly of the Over-pressure Relief Valve

  1. Remove the tubing connections to the Mixing Manifold. Use a 3/32” hex driver to remove the 2 screws holding the Mixing Manifold to the chassis. Use a 3/8” wrench to remove the end cap and discard the old Over-pressure Relief Valve Cartridge. Ultrasonicate the Mixing Manifold for at least 30 minutes. Rinse well with DI water.
      
      
     
  2. Connect the outlet of your HPLC pump to the Mixing Manifold inlet and pump 100% water at 0.5mL/min to verify the Mixing Manifold is not clogged. If the Mixing Manifold is still clogged after cleaning in an ultrasonicating bath, replace the Mixing Manifold Assembly (PN 1452-0040).
     
  3. Turn off the HPLC flow and make sure there is no pressure on the Mixing Manifold. Insert the new OPRV cartridge, green side down, and screw on the end cap to 20”lbs of torque. To approximate this level of torque, first finger tighten, then tighten an additional 1/8-1/4 turn with a 3/8” wrench.
     
  4. To verify the opening pressure of the Over-pressure Relief Valve, plug the two side inlets of the Mixing Manifold and turn on the HPLC pump to 0.5mL/min. Allow the pressure to slowly rise. The Over-pressure Relief Valve should open around 485psi. If the opening pressure is too low, tighten an additional 1/8 of a turn with a 3/8” wrench.

David Mazawa
david.mazawa@pickeringlabs.com
Technical Support Chemist
Pickering Laboratories, Inc.
1280 Space Park Way
Mountain View, CA 94043 USA
Phone: (650)694-6700 ext. 710
Fax: (650)968-0749

 

AOAC, meetings

AOAC Mid-year Meeting, SPIFAN and Tea

AOACAOAC’s 5th Annual Mid-Year Meeting was held in Gaithersburg, Maryland March 16-20. Stakeholders panels, Working groups and Expert Review panels were working on developing consensus standards, evaluating and recommending the methods as well as identifying and prioritizing issues and needs in areas of Infant and Adult nutrition, food and produce safety and Dietary supplements.  

Maria Ofitserova attended the meeting of Stakeholder Panel on Infant Formula and Adult Nutritionals (SPIFAN) where wide range of topics was discussed.  The Panel was updated on the state of methods to identify the Whey Protein content in formulas via amino acids analysis and approved Standard Method Performance Requirements (SMPRs) for Vitamins B1, B2, B3 and B6. There was a discussion about the urgent need for methods to determine Sodium Monofluoroacetate (Compound 1080) in response to threats to adulterate baby formula in New Zealand. Sodium Monofluoroacetate is used to control rodents, foxes, rabbits and other animals in Australia and New Zealand and is very toxic. The panel was able to review and approve three fit-for–purpose methods for analysis of Compound 1080. The Panel was also presented with information about harmonization of standards and methods to ensure safety and quality of infant and adult nutritional products and cooperation of AOAC with other organizations responsible for developing International Standards and Guidelines, such as CODEX and ISO.

The full-day session of SPIFAN Expert Review Panel (ERP) was dedicated to reviewing methods seeking to obtain the status of AOAC either First Action or Final Action Official Method.

AOAC Stakeholder Panel on Dietary Supplements (SPDS) launched three new working groups that will be developing Standard Method Performance Requirements for analysis of Aloin in Aloe, Vitamin D in supplements, premixes and raw materials and analysis of different components of tea, including catechins, theaflavins, and special amino acid Theanine.  Pickering is especially excited about being part of the Tea working group since we have developed an easy and robust method of analysis for Theanine and tea using cation-exchange column and post-column derivatization with Trione, Ninhydrin reagent.

Pickering Laboratories will continue working with AOAC groups to keep track of important issues in Food analysis and focus our R&D efforts.

 

artificial perspiration, sweat

Sweat, a History of and New Products Coming

sweatThe Evolution of Artificial Perspiration, Pickering-Style
By Rebecca Smith

Our employees’ favorite response to the standard “what do you do?” question plays into the novelty of some of our lesser known products:  “We make artificial sweat!” 

Coming from a traditional chromatography background (with over 30 years of reputation as the “post-column people”), it has always been a fun conversation starter, not to mention a great way to illustrate exactly how unique Pickering Labs is. 

Pickering was initially approached by the forensics industry.  Our first sweat customer, Crime Sciences Inc., inquired into the possibility of an artificial perspiration solution that would mimic a human fingerprint.  The goal was to provide a control for the fingerprinting tests used by forensic investigators.   Intrigued by the possibilities, Michael Pickering jumped on the opportunity. He began researching the chemical composition of actual human eccrine sweat.  As a result, our artificial perspiration is the only formulation standardized to be used across all industries as it mimics true human sweat and does not rely on any one desired testing result. 

After adding artificial perspiration to our catalog, we spent several years selling a bottle here and there.  Other than the catalog and webpage, we weren’t really focusing in on ‘sweat’ as a product line.  Beginning in 2011, our sweat sales unexpectedly began to take off.  We also started getting an increased number of inquiries for industry-specific artificial perspirations, with specific components designed to test one particular trait of the product.  It appeared that the time had come to expand our sweat offerings!

Today, we offer about ten different formulations of artificial perspiration (in addition to five artificial saliva formulations and three urines).  We make numerous proprietary custom formulations for our customers as well.  We have also expanded our offerings to include the ability to modify the pH or stabilization of any sweat formulation to suit specific needs a customer might have. 

Pickering continues to see increasing sales of our very popular artificial eccrine perspiration.  To accommodate growing use and facilitate larger orders, we have even begun selling our stabilized eccrine perspiration in larger bottles!  Now a customer can choose the best fit for them – 5mL, 200mL (most popular), and now 950mL. 

The unique qualities of Pickering Labs that make us so responsive to customer needs continue to thrive.  And now with our expanded product testing offerings, we are starting to be known not just as the “post-column people,” but also be recognized as “those people that make sweat” too!

It’s been a fun ride so far.  And our primary source of advertising continues to be word of mouth, so feel free to bring us up the next time you’re looking for a conversation starter!

Pittcon, Trade Shows

Pittcon 2015

Pittcon photoNew Orleans, 2015 Pittcon Exhibition –  919 exhibitors, 1,690 booths and an estimated 18,000 attendees. Pickering laboratories exhibited to share the latest products and technologies with customers, distributors and partners. There was a lot of attention to this year’s big introduction, the DEXTech Sample Clean-up system for PCBs and Dioxin. Designed to be faster, cleaner and better automated than industry standard processes the DEXTech provides Dioxin and PCB sample Clean-up at significantly lower price per run while also being faster and safer.

The Freestyle automated sample clean-up workstation highlighted the ThermElute system for Aflatoxin sample-Clean up that has a direct connection to the HPLC. Not only are the innovative Aflatoxin immunoaffinity SMART columns economical but they are incredibly fast. Now you can automate the sample clean-up and include the analysis in one setup.

One of the growth areas in the Pittcon show was the growing focus on Food Safety. Pickering laboratories continue to develop applications that focus on food safety and authentication with new methods introduced at Pittcon:

The surprise to many long time Pickering Labs customers is the introduction of the Product Test Solution line of artificial perspiration, saliva and urine. The long history with Post-Column derivatization products continues with the additional product expansion into sample clean-up products and now the products for the Product Testing laboratory market. These products build on our expertise in Amino Acid Analysis and chromatographic grade reagent manufacturing capability. Each product is manufactured in precise compliance with product testing protocols from NIST, ATSM, AATCC and numerous other standards organizations. The reliability and reproducibility of all Pickering Laboratories products is now available to the Product Testing laboratory market.

ahpa, tradeshows

AHPA User Meeting

AHPHThe 35th annual Natural Products Expo West, the world's largest natural, organic and healthy products event, took place March 4 – 8 at the Anaheim Convention Center in California. The American Herbal Products Association (AHPA), the national trade association that is focused primarily on herbs and botanicals and herbal products, held its annual member meeting and the various committees meeting during this event. This year's meeting featured a keynote speech by Josephine P. Briggs, M.D., Director of the National Center for Complementary and Integrative Health (NCCIH) at the National Institutes of Health (NIH). Her speech focused on research in the diverse medical and health care systems, practices and products that are not generally considered part of conventional medicine. The committee meetings discussed issues that are impacting herbal products industry

Chromatography, Chromatography Quiz

Chromatography Quiz #19

Chromatography Quiz #18 Results

We would like to congratulate our grand prize winners of our last newsletter’s puzzle quiz: Helene Lachance from Shur-Gain Nutreco, Tom Schneider from Suffolk County Water Authority, Narjes Ghafoori from LA County Environmental Toxicology Lab, and Joy Gottlieb from New Mexico Department of Health Scientific Lab Division!

q19-1They have each won and will shortly be receiving:
a Polaroid Cube Action Flash Memory Digital Camcorder
(POLC3X) with HD-1080p from Target.com!!

We would like to thank all of you for your submissions!

The correct answer (click to enlarge):

q19-2

Thank you!
Pickering Labs

Chromatography Quiz #19: Glyphosate Analysis High Pressure Troubleshooting!

Answer the three high pressure questions correctly and win a prize! Simply email your answer as well as your full contact information to Rebecca at rlsmith@pickeringlabs.com by May 15th, 2015 in order to win. You will receive email confirmation that your submission has been received. The answer to the puzzle and winner congratulations will be published in the next issue (to be anonymous, please notify Rebecca in submission).

Glyphosate Analysis for US EPA Method 547

HPLC running with Pinnacle PCX dual-pump system. Assume normal operating conditions (with respect to flow rates, temperatures, etc.).

Glyphosate Analysis normal pressures

Column Pressure Reagent 1 Pressure Reagent 2 Pressure
90 bar 230 psi 130 psi

After a month of analysis your pressures have changed. What is the most likely cause of the problem in each of the three scenarios below?

Scenario One:

Column Pressure Reagent 1 Pressure Reagent 2 Pressure
100 bar 430 psi 330 psi
  1. Pre-column GARD blocked
  2. Heated Reactor obstructed
  3. Ambient Reactor obstructed

Scenario Two:

Column Pressure Reagent 1 Pressure Reagent 2 Pressure
100 bar 430 psi 430 psi
  1.  Pre-column GARD blocked
  2. Heated Reactor obstructed
  3. Ambient Reactor obstructed

Scenario Three:

Column Pressure Reagent 1 Pressure Reagent 2 Pressure
180 bar 230 psi 130 psi
  1. Pre-column GARD blocked
  2. Heated Reactor obstructed
  3. Ambient Reactor obstructed