Category Archives: aflatoxin

aflatoxin, deoxynivalenol, DON, HPLC, immunoaffinity cleanup, Multi-residue Mycotoxin Analysis, mycotoxin, ochratoxin, pinnacle pcx, post-column derivatization, UVE, vomitoxin

On-Demand Webinar: Rugged and Sensitive Analysis of Mycotoxins in Food Matrices

If you missed the live webinar last summer, you can still hear the Webinar On-Demand! Just click on the links below to launch the presentation.
On August 14th, 2012, in collaboration with Thermo Scientific, Pickering Laboratories participated in a free webinar titled: Rugged and Sensitive Analysis of Mycotoxins in Food Matrices
 
The webinar was hosted by LC/GC Magazine and Thermo Scientific.
 
Live Webcast: Tuesday, August, 14, 2012 at 8:00 am PDT; 11:00 am EDT; 15:00 GMT

Register Free at http://www.chromatographyonline.com/foodmatrices
EVENT OVERVIEW
Mycotoxins are found in a staggering range of matrices, from grains to fruits, spices and nuts to animal feed. They are of major concern for their negative impact on the worldwide economy from product revenue losses, their toxicity to animals and humans, and their persistence throughout multiple food processing steps.Learn how Pickering Laboratories addresses this challenge and meets guidelines set by the FDA using sensitive and rugged Thermo Scientific UHPLC methods to effectively detect Aflatoxins as well as other key classes of Mycotoxins. These easy-to-use methods utilize immunoaffinity clean-up and post-column derivatization.

Also join us to discover the benefits of a Thermo Scientific LC-MS/MS method using automated Turbulent Flow sample extraction to separate Mycotoxins from complex food matrices. A highly efficient solution used in high throughput laboratories that eliminates manual sample preparation, improves method reliability and drives down cost per sample.

Who Should Attend
•
Analytical Chemists
•
Lab Managers
•
Food Science Researchers
Presenters
Wendy E. Rasmussen
Wendy E. Rasmussen
Technical Expert
Food, Agricultural and Environmental Markets
Pickering Laboratories
Markus M. Martin
Markus M. Martin
Solutions Manager LC/MS
Thermo Fisher Scientific
Dr. Yang Shi
Dr. Yang Shi
Senior Applications Scientist
LC/MS Applications
Thermo Fisher Scientific
Laura Bush
Moderator:
Laura Bush
Editorial Director
LCGC North America
Presented by
Sponsored by
LC/GC Logo
Thermo Logo
 
aflatoxin, deoxynivalenol, DON, eluvac, freestyle, immunoaffinity cleanup, mycotoxin, post-column derivatization, UVE, vomitoxin

New Mycotoxin Cleanup Columns!

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Also on display this year at Pittcon were the a couple of NEW! Mycotoxin clean-up columns available from Pickering Laboratories:
AflaCLEAN SMART
The immunoaffinity column AflaCLEAN SMART is designed for sample clean-up for subsequent Aflatoxin analysis. 
This columns is smaller than the other counterparts on the market – it uses only a fraction of the solvent and yet has 100ng loading capacity as specified by the AOAC methods. 
During extraction, dilution, washing, sample loading and elution, more than 80% of the solvents can be saved.

The final elution volume is only 400uL instead of the typical 2mL – and yet has the same final concentration as with other columns.  As most methods inject only 20-50uL of sample onto the autosampler, this volume is more than enough for analysis. 

These columns can also be used with the very convenient and affordable EluVac vacuum manifold.

If you would like additional information, please email us: sales@pickeringlabs.com

DONeX
The DONeX column can be used in the sample preparation for the analysis of the mycotoxin Deoxynivalenol (Vomitoxin), which is often found on contaminated cereals and grains such as corn. 
The column is ideally suited to many common matrices such as corn, barley, oat, wheat, rye or cereal-based feed. The advantage of DONex is that these columns can also be used with complex matrices and one can stll obtain excellent recovery rates. 
DONeX is used with a sample load of 1 g for HPLC / post column / fluorescence detection or LC / MS.  By increasing the matrix load up to 4 g on column, users with HPLC / UV detection systems can reach detection limits as low as 200 ppb. 
Unlike our other Mycotoxin clean-up columns, DONeX is not an immunoaffinity column, and so it can be stored on the shelf and has an undefined shelf-life.
The DONeX clean-up column is based on a 3mL standard form and thus perfectly suitable for automated handling in the LCTech systems AcceCLEANTM and FREESTLYETM SPE. 
If you are interested in any additional information or would like to try a sample, please send us an email: sales@pickeringlabs.com
AcceCLEAN, aflatoxin, Amino Acid Analysis, amino acids, antioxidants, artificial perspiration, carbamate, clean-up, deoxynivalenol, dietary supplements, elisa, eluvac, ergot alkaloid, freestyle, glyphosate, GPC, immunoaffinity cleanup, Multi-residue Mycotoxin Analysis, mycotoxin, natural toxins, Ninhydrin, ochratoxin, paralytic shellfish toxin, pinnacle pcx, post-column derivatization, psp, syringe pump, Trione, UVE, vomitoxin

Pickering Products

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Mycotoxins: Clean-up Columns, ELISA Kits, Post-Column Derivatization Instruments & Methods, SPE manifolds (manual & automated) for the analysis of:

Aflatoxins
Ochratoxin A
DON
Nivalenol
Fumonisin FB1, FB2
Zearalenone
Ergot Alkaloids

Post-Column Derivatization: Pinnacle PCX, Vector PCX, UVE Photochemical Reactor, Columns, Reagents, Eluants for the analysis of:

Antioxidants
Mycotoxins (individual and multi-residue)
Amino Acids
Biogenic Amines
Paralytic Shellfish Toxin
Polyether Antibiotics
Hexavalent Chromium
Vitamins
Sugars
Nitrate/Nitrite
Bromate
Carbamate Pesticides
Glyphosate Herbicide
NDELA
Formaldehyde

Sample Preparation (clean-up) Instruments:

FREESTYLE for SPE, GPC, online-concentration
FREESTYLE ThermELUTE for Aflatoxin analysis (direct inject onto HPLC)
AcceCLEAN for SPE (incl. Immunoaffinity columns)
EluVAC vacuum manifold for SPE (incl. Immunoaffinity columns)
GPC QUATTRO for manual GPC Cleanup
DECS System for the cleanup of samples for Dioxin analysis

Chemistry Products:

Artificial Perspiration
Artificial Saliva
High Purity Water

Stand-Alone OEM Syringe Pump:

For any applications requiring an inert flow path, volumes up to 70mL, and pressures up to 500psi

For More Information visit:

www.pickeringlabs.com

or email: sales@pickeringlabs.com

aflatoxin, AOAC

Pickering Laboratories’ Mycotoxin Workshop held at MidWest AOAC in Lincoln, NE

Sculpture on U of NE-Lincoln campus
By Maria Ofitserova, PhD
At the Midwest AOAC meeting (Held from June 6-9, 2011 in Lincoln, Nebraska) Pickering Laboratories presented a workshop on analysis of Aflatoxins in different matrices such as animal feeds, corn and peanut butter. The workshop generated a lot of interest with representatives from several State Departments of Agriculture, State Universities, as well as companies like Silliker and General Mills attending the two sessions of the workshop. The participants were able to learn about new analytical equipment and have a discussion about different aspects of Mycotoxins analysis.

During the workshop the samples extracts were cleaned with AflaClean™ Immunoaffinity columns and analyzed using an HPLC method with post-column photochemical derivatization by UVE™ and fluorescence detection. We also demonstrated how AcceClean™ workstation automates the labor-intensive column clean up step. The participants were impressed with versatility of the AcceClean workstation capable of handling multi-step procedures with both Immunoaffinity and SPE columns.
To learn more about Pickering products for Mycotoxins Analysis please visit our website http://www.pickeringlabs.com/catalog/Mycotoxin_Analysis.asp or call (800)-654-3330
aflatoxin, AOAC

Southern Section AOAC Meeting

By Wendy Rasmussen

From April 11-12, Pickering attended the Southern Section AOAC Meeting in Atlanta, Georgia. 

The meeting was well attended, and we enjoyed some good talks with current and future customers as well as long-time friends in the industry. 


In preparation for our Vendor Seminar (see below), I did some research about the southern states, and Georgia in particular. In the process, I learned a couple of interesting facts about Georgia.  For example, even though Georgia is nicknamed the Peach State, they’re only the 3rd largest producer of peaches. Turns out the state is known for its Quality rather than the quantity of the fruit. According to the internet, Georgia produces A LOT of poultry & eggs (Georgia is ranked 1st in the US for production of broilers*),  but also peanuts as well as cotton and tobacco.

We presented a Vendor Seminar at the meeting about our Aflatoxin Analysis products. We opted for a “show & tell” approach in which we brought in the key components to the method: 

UVE Photochemical Reactor

 

AflaClean Immunoaffinity Columns


and the AcceCLEAN Automated Sample Handling Workstation

The combination of these products was later demonstrated at the Mid West AOAC Workshop in Lincoln, NE



More about Georgia:
http://georgiainfo.galileo.usg.edu/gaecon.htm
http://en.wikipedia.org/wiki/Georgia_%28U.S._state%29


*Broiler: A young chicken bred for meat
aflatoxin, Chromatography Quiz

Chromatography Quiz No. 6

Chromatography Quiz #5 Results
We would like to congratulate the grand prize winner of our last newsletter’s Carbamate Chromatography Quiz: June Black from the Pennsylvania Department of Environmental Protection! 
She has won, and will shortly be receiving, an extensive California Wines Gift Basket!  Additionally, for this quiz all of our participants will each be receiving a $25 gift card from Bevmo! Again, we would like to thank you all for your submissions.  
The correct answer for the modified Carbamate chromatogram: The system was improperly equilibrated at a higher percentage of methanol (50:50 Water:Methanol) prior to injection.  After injection, the gradient program began with normal starting conditions (85:15 Water:Methanol). For the less retained compounds, the improper conditions on the column at the time of injection will push them through faster, causing coelutions.  Longer retained compounds are less affected by starting conditions of the column, and impacted more by the gradient (which was correct) so you see less of an effect on the later part of the chromatogram. 
Thank you! 
Pickering Labs
Chromatography Quiz #6:
Identify the error made when running the Aflatoxins chromatogram below and win a prize!  Simply email your answer as well as your full contact information to Rebecca at rlsmith@pickeringlabs.com by March 31st in order to win.  You will receive email confirmation that your submission has been received.  The troubleshooting answer and winner congratulations will be published in the next issue (to be anonymous, please notify Rebecca in submission). 

Aflatoxins Analysis by AOAC Official Method 2005.08
LCTech Standard: 0.25 ng B2, G2 and 1.0 ng B1, G1 injected on-column in both chromatograms
LCTech Analytical Column: 150 x 4.6 mm; RP C18 (P/N 10522)
LCTech Guard Column: 8 x 4 mm (P/N 10523; holder P/N 10750)
Normal Operating Conditions: (for reference only, condition changes may be reflected in chromatogram)
Column Temperature: 36 °C
Mobile Phase: Water/methanol/acetonitrile 55/30/15
Isocratic Flow rate: 1.3 mL/min
Post-column conditions:
Photochemical Derivatization: 254nm UV low pressure lamp (LCTech UVE derivatizer, Pickering P/N 1100-3347)
Detection: Fluorometer:  ex 365 nm, em 460 nm

AcceCLEAN, aflatoxin, AOAC, dietary supplements, GPC, herbal products, Multi-residue Mycotoxin Analysis, mycotoxin, natural products, UVE

Pickering Laboratories Introduces Key Products to Herbal Supplements Market

By Mike Gottschalk

The Supplyside West Conference in Las Vegas, Nevada October 19-23, is a very important Herbal Supplement Industry event. It provides important educational and networking opportunities to this $29 billion market. Since the FDA sent out required current good manufacturing practices (cGMP) in 2007, the Herbal Supplement producers are working to meet the new requirements before the FDA audits. These regulations are intended to insure the quality, purity and safety of the Herbal Supplement products available today. Pickering Laboratories had a booth at the show to assist the producers and laboratories with the instrumentation, chemistry, methods, and support for the analysis and clean-up of samples for Amino Acids, Aflatoxins, Carbamate pesticides, Glyphosate Herbicides, and 23 other methods.

Click on the links to see our Latest Method Abstracts, introduced at the show:
1) MA 215: Clean-up and Determination of Alfatoxins in Peanuts and Peanut Butter 

2) MA 218: Clean-up and Analysis of Aflatoxins and Ochratoxin A in Herbs and Spices 


The annual meeting of the AOAC International in Orlando, Florida on September 20-25, also had a strong emphasis on Herbal Supplements testing. The AOAC organization is central to FDA efforts to provide analytical structure to testing methods for this newly regulated industry. Pickering Laboratories participated in the AOAC sponsored Single Laboratory Validation of Multi-residue Mycotoxin Analysis in Corn. For this work, Pickering Laboratories was awarded the “Single Lab Validation of the Year”. This pivotal work in the analysis of Mycotoxins continues in Herbal Supplements as well as agricultural products.

Send us an email to support@pickeringlabs.com to request a copy of our poster: “Clean-up and Determination of Alfatoxins in Peanuts and Peanut” from the AOAC show.

Our next show is Pittcon 2011 on March 13-18 in Atlanta, Georgia.

See you there!